Preparation method of recombinant human follicle-stimulating hormone with high specific activity

Abstract

The invention relates to the technical field of follicle-stimulating hormones, in particular to a preparation method of a recombinant human follicle-stimulating hormone with high specific activity, which comprises the following steps: (1) introducing a cell line of an rhFSH / CHO-S working library into a shake flask filled with a CD Forti CHO culture medium (containing 8mM glutamine), resuscitating, shaking and culturing for 2-4 days, and when the concentration of living cell reaches 2-3*106 / ml and the activity is not less than 80%, transferring to the next stage shake flask according to the density of 2-4*105 / ml, and amplifying step by step according to the same method to complete the preparation of a seed solution; and (2) inoculating the seed solution into a bioreactor according to the density of 4-6*105 / ml of living cells, controlling the dissolved oxygen to be 30-60%, the rotating speed to be 180-220 rpm (7L bioreactor), the rotating speed to be 100-140 rpm (14L bioreactor), controlling the basic ventilation rate to be 0.001-0.01 vvm, and respectively feeding a CellTurbo Feed2 fed-batch culture medium according to the proportion (V / V) of 2-5% on the fourth, sixth, eighth and tenth days after inoculation.

Description

Technical field[0001]The present invention relates to the technical field of matrix hormones, and more particularly to a method for preparing high-specific reorganized human follicles.Background technique[0002]Follicle-Stimulating Hormone (FSH) is a glycoprotein gonadotropin secreted by pituitary pituitary leaf bacilline cells, which is a core hormone that regulates mammalian growth, sexual maturation and reproduction. FSH in the treatment of ovulation barriers, promoting the normal growth of follicles, maturation and gonad steroidal steroids, activation, and maintaining the number of normal spermia and quality, in vitro fertilization and embryo transfer pregnancy pregnancy.[0003]Currently commercial FSH preparations have a uterine source FSH (UFSH) and recombinant human pebging (RHFSH), wherein RHFSH is more in CHO cells to express a host, and it is expressed in an external secretion, and has a glycosylation configuration similar to natural FSH. The product has the advantage of hig...

AI Technical Summary

Problems solved by technology

Patent CN 110042137 A reports another method for high-density perfusion culture of recombinant CHO cells to produce human follicle-stimulating hormone. This process uses a serum-free and protein-free medium, and the culture time is maintained for 35 minutes from the inoculation of the reactor to the end of the entire perfusion culture. -52 days, the expression level ranges from 15mg-25mg / L, and the yield of protein in a single batch is more than 30g. Although the yield is considerable, too long culture time also greatly increases the risk of bacterial contamination. Storage and other production costs are high

[0006] In addition, the purification process has also become the rate-limiting step in the industrial production of rhFSH. How to efficiently obtain pure rhFSH from cell culture fluid without affecting its structure and function is another key technical problem.

Patent CN102295695 A reports the purification process route of ultrafiltration concentration, anion exchange, hydrophobicity, anion exchange, molecular exclusion, and anion exchange. The process steps are too cumbersome and the yield is low

Patent CN100554277C mentions that FSH is purified by anion exchange chromatography, fixed metal ion chromatography (ie, metal chelation chromatography) and hydrophobic chromatography, but the activity of the target protein based on the metal chelation affinity chromatography have potential adverse effects

Patents CN 103570820 A and CN 102448979 A respectively disclose dye affinity chromatography processes based on Blue Sepharose CL-6B chromatographic medium and Cibacron Blue F3G-A chromatographic medium, which have the advantages of low cost, easy control and scale-up of the process , while dye affinity chromatography has problems such as poor affinity specificity and low affinity efficiency

Images