Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS

A technology of chemical composition and pseudobulb, which is applied in the field of detection of chemical composition of Dulan pseudobulb, can solve the problems of difficult detection of chemical composition of Rhododendron orchid and uncertainty of active ingredients

Pending Publication Date: 2021-04-20
GUIZHOU UNIV
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of this, the purpose of the present invention is to provide a method based on UPLC-ESI-MS detection of chemical components in Rhododendron orchid pseudobulb extract, to overcome the problems of difficult detection of chemical components of Rhododendron orchid and uncertain active ingredients in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS
  • Method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS
  • Method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Cultivation conditions of Rhododendron orchid

[0037] Rhododendron orchid Cremastra appendiculata (D.Don) Makino was collected from Gaopo Township, Huaxi District, Guiyang City, Guizhou Province. It was planted in a sunshade greenhouse at the Experimental Farm of Guizhou University with a plant spacing of 7 cm. The cultivation medium was loose humus soil collected from broad-leaved forests. The light intensity of the greenhouse is 180-230μmol·m -2 ·s -1 , the relative humidity is 70%-85%.

[0038] (2) Extraction of chemical components of rhododendron orchid

[0039] The robust rhododendron orchid pseudobulbs that have been cultivated for 3 years were used as experimental materials, washed, cut into small pieces, dried at 60°C, crushed and passed through a 40-mesh sieve. Use 1.0 g of pseudobulb powder to ultrasonically extract three times with 70% and 55% ethanol respectively, each extraction time is 20 min, combine the extracts, and recover the ethanol until it ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a method for detecting chemical components of pseudobulbs of Cremastra appendiculata based on UPLC-ESI-MS. The method comprises the following steps: artificial cultivation conditions of the rhododendron, extraction of the chemical components of the pseudobulbs, preparation of a standard sample, preparation of a sample to be detected, liquid phase detection conditions, mass spectrum detection conditions, ion source setting and qualitative analysis of the chemical components. According to the method for detecting the chemical components of pseudobulbs of Cremastra appendiculata, 9 chemical components of colchicine, quercetin, adenosine, rutin, emodin, rheinic acid, physcion, myristic acid and oleanolic acid of the Cremastra appendiculata pseudobulb extract can be rapidly, accurately and sensitively detected; the method overcomes the defects of few chemical component detection methods, undefined effective components, difficulty in evaluating the internal quality of the medicinal material and the like, and provides guidance for evaluating the internal quality of the Cremastra appendiculata medicinal material.

Description

technical field [0001] The invention relates to a method for detecting chemical components of duran pseudobulbs based on UPLC-ESI-MS, and belongs to the field of analysis and detection of medicinal plants. Background technique [0002] Rhododendron orchid (Cremastra appendiculata (D.Don) Makino) is a rare perennial medicinal plant of the genus Rhododendron orchid in the family Orchidaceae. "Chinese Pharmacopoeia records: Arrowhead mushroom is the dry pseudobulb of Rhododendron orchid, Suanlan and Yunnan Suanlan, all of which have the effects of clearing away heat and detoxification, moisturizing the lungs and relieving cough, promoting blood circulation and removing blood stasis, reducing swelling and dissipating stagnation, etc. Oral administration can be used for gastric cancer, Liver cancer, breast cancer, uterine cancer, etc. External use can cure skin burns, snake bites, etc. Among the three, Rhododendron orchid has the best quality and the most research. [0003] The...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 杨宁线张明生王道平胡恩明李术钗高燕燕彭思静
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com