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Method for improving capture accuracy of circulating tumor cells and microfluidic material

A tumor cell and precision technology, applied in the field of medical materials, can solve problems such as false positives, achieve the effect of improving accuracy and capture accuracy

Active Publication Date: 2021-04-30
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This deterministic lateral displacement technology can also effectively capture tumor cells, but it is prone to false positive results and needs further improvement

Method used

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  • Method for improving capture accuracy of circulating tumor cells and microfluidic material
  • Method for improving capture accuracy of circulating tumor cells and microfluidic material
  • Method for improving capture accuracy of circulating tumor cells and microfluidic material

Examples

Experimental program
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Effect test

preparation example Construction

[0023] S1. Preparation of polydimethylsiloxane chip

[0024] Please combine figure 1 , making a polydimethylsiloxane (PDMS) chip with a micropillar array structure on a silicon wafer template, and doping a conductive material in the polydimethylsiloxane chip to obtain a conductive PDMS microchip. The microarray structure formed by PDMS is used to form a microfluidic chip to capture circulating tumor cell CTCs. The addition of conductive materials enables the materials to deposit electrospun fibers by electrospinning.

[0025] It should be noted that under the conventional parameter conditions, the size of the micro-pillars is relatively large, mainly due to the obstruction of the micro-pillars to the fluid, which causes the shunt to flow laterally, and produces an uneven velocity field distribution on the surface of the micro-pillars, making The particles therein have more movement trajectories and more collisions with the micro-pillars in the three-dimensional space on the m...

Embodiment 1

[0048] This embodiment provides a method for improving the accuracy of capturing circulating tumor cells, which includes the following steps:

[0049] (1) Take a clean silicon wafer template, blow it off with nitrogen, place it in a petri dish with a flat bottom, and then perform silanization treatment (drop 3 drops of hexamethyldisilazane on the silicon substrate, wait until the volatilization is complete, and drop it again , repeated 3 times). Next, weigh a certain amount of PDMS prepolymer and tetraethyl orthosilicate at a mass ratio of 10:1, mix for about 2 minutes, and pour it into a petri dish with a silicon template to control the thickness of PDMS to about 3.5mm. Place the petri dish with PDMS and silicon template in a vacuum drying oven, vacuum for 2 minutes to remove air bubbles, then let it stand at room temperature for 15 minutes, and then transfer it to a constant temperature drying oven at 80°C for about 60 minutes to cure crosslinking. Peel off the cured PDMS ...

Embodiment 2

[0054] This embodiment provides a method for improving the accuracy of capturing circulating tumor cells, which includes the following steps:

[0055] (1) Take a clean silicon wafer template, blow it off with nitrogen, place it in a petri dish with a flat bottom, and then perform silanization treatment (drop 4 drops of hexamethyldisilazane on the silicon substrate, wait until the volatilization is complete, and drop it again , repeat 2 times). Next, weigh a certain amount of PDMS prepolymer and phenyltrimethoxysilane according to the mass ratio of 8:1, mix for about 2 minutes, pour it into a petri dish with a silicon template, and control the thickness of PDMS to about 4.5mm. Place the petri dish with PDMS and silicon template in a vacuum drying oven, vacuum for 2 minutes to remove air bubbles, then let it stand at room temperature for 20 minutes, and then transfer it to a constant temperature drying oven at 80°C for about 60 minutes to cure crosslinking. Peel off the cured ...

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Abstract

The invention discloses a method for improving capture accuracy of circulating tumor cells and a microfluidic material, and relates to the technical field of medical materials. The method for improving the capture accuracy of the circulating tumor cells comprises the following steps: manufacturing a polydimethylsiloxane chip with a micro-pillar array structure on a silicon wafer template, and carrying out conductive treatment on the polydimethylsiloxane chip to obtain a conductive PDMS microchip; depositing electrospun fibers on the surface of the conductive PDMS microchip in an electrospinning mode to obtain an electrospun fiber modified microchip; and modifying the surface of the electrospun fiber modified microchip with one or more antibodies by introducing disulfide bonds. The micro-fluidic material for capturing the circulating tumor cells comprises a polydimethylsiloxane micro-pillar array, electrospun fibers are deposited on the micro-pillar array, antibodies are modified on the surfaces of the electrospun fibers through disulfide bonds, CTCs can be captured more accurately, and the CTCs can be completely released.

Description

technical field [0001] The invention relates to the technical field of medical materials, and in particular to a method for improving the capture accuracy of circulating tumor cells and a microfluidic material. Background technique [0002] In tumor metastasis, all kinds of tumor cells that come off from the primary tumor and enter the blood circulation, that is, circulating tumor cells (CTCs), are widely considered to be closely related to tumor metastasis. The detection of CTCs is a minimally invasive detection, which requires only a small amount of blood from the human body, and has little damage to the human body. At present, the corresponding CTCs have been found in the blood of cancer patients including lung cancer, prostate cancer, breast cancer, colon cancer and pancreatic cancer, but not in the blood of healthy humans. With the deepening of research, the number of CTCs in cancer patients has been considered as an important indicator for judging whether cancer has m...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/00C12N5/09B81C1/00
CPCB01L3/502707B01L3/502761C12M23/16C12M47/04C12N5/0693B81C1/00214C12N2509/00B01L2300/12
Inventor 杨光蒋正鑫曹敏周绍兵
Owner SOUTHWEST JIAOTONG UNIV