Characteristic spectrum identification method for carbonized sanguisorba root formula granules
A technology of formula granule and characteristic map, which is applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of high precision, good stability and simple method
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0053]Preparation of reference solution Take 0.5g of Burnet reference medicinal material (batch number: 121286-201703), put it in a stoppered Erlenmeyer flask, add 50mL of water, ultrasonicate for 30 minutes, let cool to room temperature, shake well, filter, and take continued The filtrate was used as the reference substance solution of the contrast medicinal material. Take another appropriate amount of ellagic acid reference substance, weigh it accurately, put it in a brown measuring bottle, add 70% methanol to make a solution containing 30μg per 1mL.
[0054] Preparation of the test solution: Take 0.1 g of this product (batch number: GR-DYT-01), accurately weigh it, place it in a stoppered Erlenmeyer flask, add 50 mL of water accurately, weigh it, and ultrasonically process it (power 600W, frequency 40kHz ) for 30 minutes, let cool to room temperature, weigh again, make up for the lost weight with water, shake well, filter, and take the filtrate to obtain the final product. ...
Embodiment 1
[0134] In summary, the RSD of the relative retention time of each characteristic peak meets the requirements in the above investigations, and the method is good. The above nine characteristic peaks were included in the follow-up investigation.
[0135] Verification results of 3 batches of Burnet charcoal formula granules
[0136] The proposed method was used to measure the characteristic spectra of 3 batches of samples of this product, and calculate the relative retention time and relative peak area. Such as Figure 14 , Table 14 and Table 15.
[0137] Table 14 Relative retention time of 3 batches of Burnet charcoal formula granules
[0138]
[0139] Table 15 Relative peak areas of three batches of Burnet charcoal formulation particles
[0140]
[0141] According to the principles of stable relative retention time and relatively high peaks that can be detected in all batches of samples, a total of 9 peaks with good repeatability were selected as characteristic peaks....
Embodiment 2
[0143] Establishment of Specified Value Limits for Relative Retention Time
[0144] See Table 16 and Table 17 for a summary of the methodological inspection items and verification results:
[0145] Table 16 Summary standard of RSD% of each project result of methodology—relative retention time
[0146]
[0147] Table 17 Summary standard of RSD% of methodological project results—relative peak area
[0148]
[0149] It can be seen from the above table that the flow rate has the greatest influence on the relative retention time of the characteristic peaks, so the flow rate is set at 1mL / min. In order to increase the reproducibility and applicability of the method, the relative retention time of each peak is tentatively set at 8%. .
[0150] Final regulation: there should be 9 characteristic peaks in the characteristic spectrum of the test product, among which the peak corresponding to the ellagic acid reference substance is the S peak, and the relative retention time of ea...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Column length | aaaaa | aaaaa |
| The inside diameter of | aaaaa | aaaaa |
| Particle size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More