Preparation method of snakehead epidermal mucus antibacterial peptide

Abstract

The invention belongs to the technical field of biological extraction, and relates to a preparation method of a snakehead epidermal mucus antibacterial peptide. The preparation method comprises the following steps: collecting mucus of the back epidermis of snakeheads, heating in a water bath, cooling, centrifuging and collecting supernate; after pre-freezing, freeze-drying the supernate to obtain freeze-dried powder of the snakehead epidermal mucus; purifying the freeze-dried powder by adopting a Sephadex G-100 gel column, then collecting a product, detecting a light absorption value at 280 nm, collecting a component, and freeze-drying to obtain a freeze-dried component; and further purifying by adopting a cation exchange column, collecting a product, detecting a light absorption value at 280 nm, finally collecting a component, dialyzing, desalting and freeze-drying to obtain the snakehead epidermal mucus antibacterial peptide. The method is simple in process and relatively low in cost, has a good inhibition effect on gram-positive bacteria and gram-negative bacteria, has broad-spectrum antibacterial activity, can be used for preparing a fish preservative or a feed additive, and is beneficial to realizing high-value utilization of snakehead processing byproducts.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for preparing an antibacterial peptide from snakehead epidermis mucus. Background technique [0002] Antimicrobial peptides (AMPs) are a class of polypeptides with antibacterial activity widely distributed in mammals, amphibians, insects or other invertebrates, also known as host defense peptides, and are important components of the innate immune system part. AMPs typically range in length from 10 to 50 amino acids, have a net positive charge of 2 to 8, and exhibit broad activity against a variety of microorganisms, including Gram-positive and Gram-negative bacteria, fungi, viruses, and parasites, and are highly toxic Little or no toxicity. Antimicrobial peptides have the advantages of thermostability, broad-spectrum antibacterial, and resistance to drug resistance. Studies have found that they have the potential to replace traditional antibio...

AI Technical Summary

Problems solved by technology

Chemical synthesis is currently a relatively common method in the production of antimicrobial peptides, but the high cost of artificially synthesized antimicrobial peptides makes it difficult to use them in batches in low-cost production such as living and breeding

Commonly known as snakehead, snakehead snakehead is a rare freshwater fish with high economic value and nutritional and medical value. With the development of snakehead aquaculture, a large amount of mucus produced during the processing of snakehead snakehead has not been effectively utilized, which wastes biological resources and damages the The ecological environment has created tremendous pressure; therefore, finding a simple and efficient extraction method to extract antibacterial substances from the mucus of the snakehead skin is an urgent technical problem that needs to be solved, which will help improve the processing and comprehensive utilization of by-products of the snakehead, and achieve high waste materials. value utilization, reduce environmental pollution, and promote the sustainable development of snakehead

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