Application of 1,2,3,4,6-o-pentagalloylglucose in the preparation of medicaments for preventing and/or treating pulmonary fibrosis
A technology of pentagalloyl glucose and pulmonary fibrosis, which is applied in the field of medicine, can solve the problems that have not yet been treated, and achieve the effect of no toxic side effects and good resistance to pulmonary fibrosis
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Embodiment 1
[0072] Example 1 Inhibitory effect of 1,2,3,4,6-O-pentagalloglucose on the proliferation of lung fibroblasts
[0073] 1. Experimental method
[0074] Lung fibroblasts were purchased from Fenghui Biological Cell Company.
[0075] (1) Cell culture
[0076] Lung fibroblasts were cultured in high-glucose DMEM medium containing 10% fetal bovine serum, 1% penicillin and streptomycin double antibody, and glucose concentration 4.5g / L, and the cells were placed at 37°C containing 5% CO. 2 Incubator, change the cell culture medium every 2d. When the cell density is 80% to 90%, trypsinize and resuspend the cells for passage.
[0077] (2) Administration
[0078] After observing that the cells grow well, the cells were digested and resuspended, and the fibroblasts were seeded on a black 96-well plate at a density of 3000 cells per well. 24h after the cells were seeded, TGF-β at a concentration of 10ng / mL was added to each well 1 to establish a cellular pulmonary fibrosis model. Afte...
Embodiment 2
[0090] Example 2 1,2,3,4,6-O-pentagalloylglucose anti-pulmonary fibrosis test
[0091] 1. Experimental method
[0092] (1) SPF grade Wistar rats were randomly divided into 5 groups according to body weight, with 30 rats in each group. sham operation group, model group, 1,2,3,4,6-O-pentagalloylglucose high dose (500mg / (kg d)), medium dose (250mg / (kg d)) and low dose, respectively Dosage group (125 mg / (kg·d)).
[0093] (2) Model preparation
[0094] After the experimental animals were purchased, they were routinely raised and adapted to the environment for 7 days. During the experiment, the animals were anesthetized by intraperitoneal injection of 3 mL / kg of 10% trichloroacetaldehyde hydrate, and they were fixed on the mouse board in supine position, and their necks were sheared. The skin is routinely disinfected, and a mid-neck incision of about 0.5 to 1 cm in length is made under aseptic operation. Peel off the exposed trachea layer by layer, and then raise the head end o...
Embodiment 3
[0103] Example 3 1,2,3,4,6-O-pentagalloylglucose acute toxicity test
[0104] 1. Experimental method
[0105] Take the acute toxicity maximum tolerated dose method for the test. A blank group and a 1,2,3,4,6-O-pentagalloglucose (1.5g / mL) group were set up, a total of 2 groups, and 60 quarantine-qualified SPF ICR mice, half male and female, were selected. Gender and weight were randomly divided into two groups by segment, 30 animals in each group, fasted for more than 12 hours before administration, the blank group was not administered, and the drug group was given 0.4ml / 10g during the test. 体重 One-time intragastric administration. On the day of administration, especially within 0 to 4 hours after administration, closely observe and record the poisoning manifestations and characteristics of the animals in each group, the occurrence and recovery time of toxic reactions, and the death situation. Then observe twice a day, namely once in the morning and once in the afternoon, fo...
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