Application of 1, 2, 3, 4, 6-O-pentagalloylglucose in preparation of medicine for preventing and/or treating pulmonary fibrosis
A technology for pentagalloyl glucose and pulmonary fibrosis, which is applied in the field of medicine and can solve problems that have not yet been treated
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Embodiment 1
[0072] Example 1 Inhibitory Effect of 1,2,3,4,6-O-Pentagalloyl Glucose on the Proliferation of Lung Fibroblasts
[0073] 1. Experimental method
[0074] Lung fibroblasts were purchased from Fenghui Biocell Company.
[0075] (1) Cell culture
[0076] Lung fibroblasts were cultured in high-glucose DMEM medium containing 10% fetal bovine serum, 1% penicillin and streptomycin, and glucose concentration 4.5g / L, and the cells were placed at 37°C with 5% CO 2 Incubator, replace the cell culture medium every 2 days. When the cell density was 80%-90%, the cells were digested with trypsin and resuspended for passage.
[0077] (2) Administration
[0078] After observing that the cells were growing well, the cells were digested and resuspended, and the fibroblasts were seeded on a black 96-well plate at a density of 3000 cells per well. 24 hours after cell seeding, add TGF-β at a concentration of 10 ng / mL to each well 1 , to establish a cellular lung fibrosis model. After culturing...
Embodiment 2
[0090] Example 2 1,2,3,4,6-O-pentagalloyl glucose anti-pulmonary fibrosis experiment
[0091] 1. Experimental method
[0092] (1) SPF grade Wistar rats were randomly divided into 5 groups according to body weight, 30 rats in each group. They were sham operation group, model group, 1,2,3,4,6-O-pentagalloyl glucose high dose (500mg / (kg·d)), medium dose (250mg / (kg·d)) and low dose Dosage group (125mg / (kg·d)).
[0093] (2) Model preparation
[0094] After the experimental animals were purchased, they were reared routinely and adapted to the environment for 7 days. During the experiment, the animals were anesthetized by intraperitoneal injection of 3 mL / kg of 10% chloral hydrate, made to lie supine and fixed on the mouse board, and the hair on the neck was clipped. The skin was routinely disinfected, and a mid-neck incision about 0.5-1 cm in length was made under aseptic operation. Peel off the exposed trachea layer by layer, and then raise the head of the mouse board so that ...
Embodiment 3
[0103] Example 3 1,2,3,4,6-O-pentagalloyl glucose acute toxicity test
[0104] 1. Experimental method
[0105] The acute toxicity maximum tolerated dose method was used for the test. Set up a blank group and a 1,2,3,4,6-O-pentagalloyl glucose (1.5g / mL) group, a total of 2 groups, select 60 quarantine-qualified SPF-grade ICR mice, half male and half female, press Gender and body weight were randomly divided into two groups with 30 rats in each group, fasting for more than 12 hours before administration, no administration in the blank group, and 0.4ml / 10g in the drug group during the test. 体重 Perform one-time gavage administration. On the day of administration, especially within 0-4 hours after administration, closely observe and record the poisoning manifestations and characteristics, occurrence and recovery time of toxic reactions, and death of animals in each group. Then observe twice a day, that is, once in the morning and once in the afternoon, and observe continuously f...
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