Fermentation preparation method of Aureobasidin A and culture medium of Aureobasidin A
A fermentation medium and fermentation culture technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of less production technology of AureobasidinA
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0098] Embodiment 1.AureobasidinA fermentation preparation
[0099] Take the working strain glycerol tube of strain CGMCC NO.20887, after thawing, take two rings of bacterial suspension to inoculate solid medium, incubate at 25°C for 72 hours, take one ring of bacterial lawn to inoculate seed medium, incubate at 25°C for 48 hours, then inoculate with 1% The inoculum is inoculated into the fermentation medium, the fermentation temperature is 25°C, the air flow rate is 1vvm, and the stirring is 100rpm. After culturing for 56 hours, add fresh feeding medium with 1 / 5 of the initial feeding volume, and continue to operate at 25°C, the air flow rate is 1vvm, and stirring is 100rpm. , cultivated to the end of 8 days to obtain a fermented liquid containing AureobasidinA. Take 1ml of the fermentation broth, soak it with 5 times the volume of absolute ethanol for more than half an hour, centrifuge to get the supernatant, filter it with a 0.45 μm membrane, and analyze and detect it by HP...
Embodiment 2
[0103] Example 2. AureobasidinA fermentation preparation method optimization
[0104] Strain activation: take the glycerol tube of the working strain, thaw it in a water bath at 30°C, mix well, take 0.1ml of the bacterial suspension and inoculate it on a PDA medium plate, spread it evenly with a coating stick, and wrap it upside down at 22-30°C. After activating and culturing in an incubator with a relative humidity of 40-70% for 4 days, the bacterial lawn is thick and full, and it is creamy or olive green.
[0105] Bacterial suspension preparation: take well-grown and qualified plate bacterial lawn, wash each plate with 15ml sterile physiological saline, transfer to a triangular flask with two spoonfuls of glass beads, shake for 30min, disperse and mix well to obtain bacterial suspension.
[0106] Seed expansion: Take the prepared bacterial suspension, inoculate it into the seed medium of the shake flask with an inoculum amount of 0.5%, and cultivate it on a shaker at 25° C....
Embodiment 3
[0111] Embodiment 3.AureobasidinA seed formulation optimization
[0112] Strain activation: take the glycerol tube of the working strain, thaw it in a water bath at 30°C, mix well, take 0.1ml of the bacterial suspension and inoculate it on a PDA medium plate, spread it evenly with a coating stick, and wrap it upside down at 22-30°C. After activating and culturing in an incubator with a relative humidity of 40-70% for 4 days, the bacterial lawn is thick and full, and it is creamy or olive green.
[0113] Bacterial suspension preparation: take well-grown and qualified plate bacterial lawn, wash each plate with 15ml sterile physiological saline, transfer to a triangular flask with two spoonfuls of glass beads, shake for 30min, disperse and mix well to obtain bacterial suspension.
[0114] Seed expansion: Take the prepared bacterial suspension, inoculate it into the optimized shake flask seed medium with an inoculum amount of 0.5%, and cultivate it on a shaker at 25°C and 220rpm fo...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More