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Colloidal gold detection kit for sodium picosulfate, bisacodyl and deacetylated bisacodyl and application of colloidal gold detection kit

A detection device and antibody technology, applied in the field of colloidal gold detection kits for bisacodyl and deacetylbisacodyl, and sodium picosulfate, can solve the problems of difficult on-site rapid detection, long detection time, cumbersome operation, etc., and achieve Economical, fast, low cost and easy to use

Pending Publication Date: 2021-05-25
广东省药品检验所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are qualitative and quantitative accurate, but the operation is cumbersome and the inspection time is long, it is difficult to achieve rapid on-site detection, and cannot provide technical support for on-site evidence collection and law enforcement

Method used

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  • Colloidal gold detection kit for sodium picosulfate, bisacodyl and deacetylated bisacodyl and application of colloidal gold detection kit
  • Colloidal gold detection kit for sodium picosulfate, bisacodyl and deacetylated bisacodyl and application of colloidal gold detection kit
  • Colloidal gold detection kit for sodium picosulfate, bisacodyl and deacetylated bisacodyl and application of colloidal gold detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059]Example 1 Preparation of a diphenylmethane drug semi-antigen

[0060]1. Add the raw material phenol 12.5 g to the single-mouth flask heating, so that 8 ml of dichloromethane is mixed after melt is mixed, and the temperature is lowered to 15 ° C or lower. The pyridine-2-formaldehyde was slowly added dropwise to 5.0 g (the temperature of the dripping process should not exceed 15 ° C). After the dropwise addition was completed, it continued to crystallize to 0.9 g of concentrated sulfuric acid (5 ° C). The reaction was reacted at 0 ° C for 1 h after completion of the mixture, then warmed to the room temperature reaction for 21 h. After the reaction was completed, the temperature was lowered to 0 ° C and the pH to 7 was adjusted with ice-cold 10% sodium hydroxide. The mixture was continued for 0.5 h to ensure that the pH did no longer changing 30 ml of ethanol. Continue to stir to have a large amount of solid precipitation. Filter, the filter cake was washed with a small amount of wa...

Embodiment 2

[0065]Example 2 Synthesis of a diphenylmethane drug immune antigen

[0066]An immunogenic antigen was prepared using a diphenylmethane drug semi-antigen from which was obtained in Example 1. Take the diphenylmethane drug semi-antigen 0.1 mmol dissolved in 2 mLDMF, stirred and added 0.2 mmol DCC and 0.15 mmol NHS. The magnetic stirring reaction was stirred at 4 ° C overnight, and the supernatant was a liquid after centrifugation, and the blood blue protein (KLH) 140 mg was dissolved in PBS (pH 8.0) of 10 ml concentration of 0.1 mol / L. DMF1ML was added, stirred and dissolved, prepared a B liquid, magnetic stirring, and the A liquid gradually added to the B liquid, and at 4 ° C for 12 h. After centrifugation, the supernatant was taken, 4 ° C was dialyzed with physiological saline for 3 days, and 3 dialysate were replaced, the total antigens obtained were distributed in a 0.5 ml of centrifuge tube at a concentration of 1 mg / ml. Frozen in the -20 ° C refrigerator.

Embodiment 3

[0067]Example 3 Preparation of a diphenylmethane drug monoclonal antibody

[0068]The monoclonal antibody was prepared using a diphenylmethane drug immunogenic antigen in Example 2. Using diphenylmethane immune antigen and identified 4 6-week old BALB / C mice, enhanced immunization, blood test, blood test, no longer increase, use twice doses of antigen The immunized mice were immunized. After three days, the mouse was subjected to the spleen to prepare spleen cells in sterile conditions, and the proportion of mouse myeloma and mixed with the growth of 8: 1 was mixed with 50 ml of centrifuge tube, add 30 ml of serum IPMI1640. The medium, 1100r / min centrifuge 5 min abandoned, and gently loudly, placed in a 37 ° C water bath. The 1 ml50% PEG-4000 slowly added cells, dripped within 1 min, and gently stir the bottom precipitate, then allowed for 1 min, the first 30s slowly eliminated the serum-free medium 1 ml of the tube wall, then add 2 ml, then Quickly add 27ml termination fusion proc...

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Abstract

The invention discloses a novel compound. By adopting the compound, the recognition sensitivity and specificity of sodium picosulfate, bisacodyl and deacetylated bisacodyl can be improved. A chromatographic immune colloidal gold principle is applied, the compounds are made into antigens, a detection device is further formed, the detection device comprises a test strip and a reaction cup, and a detection line and a quality control line in the test strip are used for semi-quantitative detection of the content of sodium picosulfate, bisacodyl and deacetylated bisacodyl in a sample through line colorimetry. According to the invention, whether a sample contains sodium picosulfate, bisacodyl and deacetylated bisacodyl or not can be rapidly and accurately detected within a short time, the detection requirement of illegally adding sodium picosulfate, bisacodyl and deacetylated bisacodyl in weight-losing products can be met, and the requirement of on-site supervision and law enforcement of supervision departments and detection institutions can be met. Compared with the prior art, the kit has the characteristics of convenience in use, economy, quickness, easiness in manufacturing and low cost.

Description

Technical field[0001]The present invention belongs to the field of colloidal gold detection, and is specifically related to sodium sulfate, siscipate and deacetyl than Saibi gemmid body gold test kit and its application thereof.Background technique[0002]In recent years, the illegal addition of food, health food, and medium medicine have emerged, which claims that products with weight loss, slimming, and safe passing have become illegally added, and new illegal additives have emerged, and they have always been analyzed. Research hotspots.[0003]Sodium can sulfate, Bischii and deacetyl Pishai are diphenylmethane irritating laxatives, by stimulating the sensory nerve endips of the colorectal mucosa, enhances intestinal peristalsis and intestinal secretion. After the sodium sulfate and Bishatidine are intraced in the intestinal tract to be a deacetylase or colon bacterial water to the active bisphenol, promote the acceleration of colon peristalsis, reduce transmission time and promote mo...

Claims

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Application Information

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IPC IPC(8): C07D213/30C07K16/44C07K14/795C07K14/765G01N33/558G01N33/543G01N33/53
CPCC07D213/30C07K16/44C07K14/795C07K14/765G01N33/94G01N33/558G01N33/54346
Inventor 温家欣何嘉雯王炳志杨星星罗卓雅赖宇红刘亚雄严义勇付辉
Owner 广东省药品检验所
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