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Method for determining concentration of clindamycin in plasma by liquid chromatography-mass spectrometry

A technology of clindamycin and clindamycin, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, selectivity, etc. to be improved, and achieve the effects of high sensitivity, fast method and easy operation

Inactive Publication Date: 2021-05-25
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the speed, precision, sensitivity and selectivity of the existing clindamycin assay methods need to be improved

Method used

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  • Method for determining concentration of clindamycin in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of clindamycin in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of clindamycin in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Example: Human K 2 Determination of Clindamycin Concentration in EDTA Plasma

[0032] 1. Experimental materials and analytical equipment

[0033] Clindamycin (analyte): China National Institutes for Food and Drug Control or the same or higher grade standard Clindamycin-d3 (internal standard): Toronto Research Chemicals or the same or higher grade standard Reagents used see Table 1 below:

[0034] Table 1 Reagent Details

[0035] Reagent name level manufacturer Acetonitrile (ACN) HPLC J. T. Baker Formic acid (FA) ACS Adamas Methanol (MeOH) HPLC J. T. Baker

[0036] Note: Reagents of the same grade or higher can also be used

[0037] The analytical equipment used is shown in Table 2 below:

[0038] Table 2 Details of equipment used

[0039]

[0040]

[0041] The same LC / MS / MS system can also be used.

[0042] 2. Liquid conditions

[0043] 1. Liquid chromatography conditions

[0044] Columns are Synergi TM 4μm P...

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PUM

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Abstract

The invention discloses a method for determining the concentration of clindamycin in plasma by liquid chromatography-mass spectrometry. According to the method, a liquid chromatography-mass spectrometry system is adopted for determination; a to-be-detected sample is taken, a certain amount of mixed organic solvent is added for extraction, and after pretreatment, separation is performed through a chromatographic column; and detection is performed through a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for determining the blood concentration of the clindamycin; according to the method, the linear range of a plasma standard curve is 50-5000 ng / mL, the intra-batch and inter-batch precision RSD is smaller than + / -15%; and the method is suitable for measuring the concentration of the clindamycin in the plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a method for determining the concentration of clindamycin in blood plasma by liquid mass spectrometry. Background technique [0002] Clindamycin Hydrochloride Capsules are suitable for the following infections caused by sensitive strains such as Streptococcus, Staphylococcus and anaerobic bacteria: otitis media, sinusitis, chemical phlogiston, pneumonia; skin and soft tissue infection; Treatment of bone and joint infection, abdominal infection, pelvic infection, chest, lung swelling, osteomyelitis, sepsis, etc., can be used alone or in combination with other antibacterial drugs according to the situation. [0003] Clindamycin plays an antibacterial role by acting on the 50S subunit of the ribosome of sensitive bacteria, preventing the extension of the peptide chain, and inhibiting the protein synthesis of bacterial cells. It can enhance the neuromuscular blockade o...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范志鸿
Owner 徐州立顺康达医药科技有限公司
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