Immobilized yeast cell microspheres as well as preparation method and application thereof

A technology for immobilizing yeast and yeast cells, which is applied in the field of immobilized yeast cell microspheres and its preparation, can solve the problems of low embedding efficiency of immobilized yeast cells, difficulty in recovering phenylethyl alcohol, and large loss of cell viability, etc. Embedding efficiency, high embedding efficiency, and small loss of cell viability

Active Publication Date: 2021-05-28
WANHUA CHEM GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is that the embedding efficiency of existing immobilized yeast cells is low (generally only up to 80%), the loss of cell viability is large, the recovery process of the final fermentation product phenylethanol is difficult, and the energy consumption is large.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] 1. Bacteria culture

[0052] YPD medium was prepared and sterilized at 121°C for 20 minutes, and Saccharomyces cerevisiae stored at -20°C was inoculated into the YPD medium, and cultured at 30°C and 150 rpm for 16 hours with shaking. The culture medium was transferred to a centrifuge tube, centrifuged at 8000rpm for 10min, and the upper culture medium was discarded. Add sterile water to resuspend the yeast cells, centrifuge again, and wash with sterile water for 3 to 4 times to obtain the cleaned yeast.

[0053] 2. Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0054] Weigh the ferric salt FeCl with a feeding molar ratio of 3:1 on weighing paper 3 ·6H 2 O and ferrous salt FeCl 2 4H 2 O, was dissolved in 100mL ultrapure water to obtain an iron salt solution with a total iron content of 0.4mol / L, and kept mechanically stirring in a three-necked flask. Prepare 1mol / L NaOH aqueous solution, slowly add it dropwise to the iron salt solution under the protection of n...

Embodiment 2

[0061] 1. Bacteria culture

[0062]YPD medium was prepared and sterilized at 121°C for 20 minutes at high temperature. Saccharomyces cerevisiae stored at -20°C was inoculated into YPD medium and incubated at 30°C and 150 rpm for 24 hours with shaking. The culture medium was transferred to a centrifuge tube, centrifuged at 8000rpm for 10min, and the upper culture medium was discarded. Add sterile water to resuspend the yeast cells, centrifuge again, and wash with sterile water for 3 to 4 times to obtain the cleaned yeast.

[0063] 2. Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0064] Weigh the ferric salt FeCl with a feeding molar ratio of 2:1 on weighing paper 3 ·6H 2 O and ferrous salt FeCl 2 4H 2 O, was dissolved in 100mL ultrapure water to obtain an iron salt solution with a total iron content of 0.4mol / L, and kept mechanically stirring in a three-necked flask. Prepare 1mol / L NaOH aqueous solution, slowly add it dropwise to the iron salt solution under the prot...

Embodiment 3

[0071] 1. Bacteria culture

[0072] YPD medium was prepared and sterilized at 121°C for 20 minutes. Saccharomyces cerevisiae stored at -20°C was inoculated into YPD medium, and cultured at 30°C and 250 rpm for 16 hours with shaking. The culture medium was transferred to a centrifuge tube, centrifuged at 8000rpm for 10min, and the upper culture medium was discarded. Add sterile water to resuspend the yeast cells, centrifuge again, and wash with sterile water for 3 to 4 times to obtain the cleaned yeast.

[0073] 2. Fe 3 o 4 Preparation of Magnetic Nanoparticles

[0074] Weigh the ferric salt FeCl with a feeding molar ratio of 2:1 on weighing paper 3 ·6H 2 O and ferrous salt FeCl 2 4H 2 O, was dissolved in 100mL ultrapure water to obtain an iron salt solution with a total iron content of 0.4mol / L, and kept mechanically stirring in a three-necked flask. Prepare 0.7mol / L NaOH aqueous solution, and slowly add it dropwise to the mixed solution of iron salt under the protecti...

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PUM

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Abstract

The invention discloses immobilized yeast cell microspheres as well as a preparation method and an application thereof. The immobilized yeast cell microsphere disclosed by the invention is of a balloon-shaped structure, a shell is an embedding material, the interior is a core material containing yeast cells, and the embedding material is a product obtained by crosslinking glyceraldehyde and a polyglycine-serine block copolymer. The immobilized yeast cell microspheres provided by the invention are high in embedding efficiency and small in cell activity loss, and can realize rapid separation of fermentation products when the immobilized yeast cell microspheres are used for fermentation production of phenethyl alcohol, and the immobilized yeast cell microspheres can be reused, so that the production efficiency of phenethyl alcohol is greatly improved, the production cost is reduced, and the method has a wide application prospect in the field of fermentation.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an immobilized yeast cell microsphere, a preparation method and application thereof. Background technique [0002] Cell immobilization technology refers to the fixing of microbial cells on a certain carrier by physical or chemical means. The carrier has a certain degree of hydrophilicity but is insoluble in water. After the bacteria are fixed, normal Physiological metabolism not only maintains the catalytic and preparation activity of the bacteria itself, but also can be recycled many times, and the bacteria can also maintain high stability in continuous operation. Therefore, the method is an important means for reducing production costs, controlling the reaction process, preventing bacterial contamination in the fermentation process, and rapidly separating the product from the bacteria. Immobilized cell technology is developed on the basis of immobilized enzymes, which retains the na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/096C12N11/14C12P7/22C12R1/865
CPCC12N11/08C12N11/14C12P7/22Y02E50/10
Inventor 单雨瑶王竞辉张雅萍杨付伟王坤黎源孔令晓王庆义周芸
Owner WANHUA CHEM GRP CO LTD
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