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An electrochemical sensor for detecting ciprofloxacin and its detection method

A ciprofloxacin and sensor technology, applied in the direction of material electrochemical variables, resistance to vector-borne diseases, etc., can solve the problems of electrode modification materials and complex synthesis process, the influence of interfering substances cannot be ruled out, and the accuracy of detection results is reduced. , to achieve low cost, increase current value, and improve accuracy

Active Publication Date: 2022-06-07
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the structural analogs of ciprofloxacin appear in the tested sample matrix, the method reported above cannot exclude the influence of interfering substances, which will reduce the accuracy of the test results
In addition, the electrode modification materials and synthesis process used in the above reported method are relatively complicated, and high temperature heating is also required.

Method used

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  • An electrochemical sensor for detecting ciprofloxacin and its detection method
  • An electrochemical sensor for detecting ciprofloxacin and its detection method
  • An electrochemical sensor for detecting ciprofloxacin and its detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0070] 1. Electrochemical sensor electrode modification:

[0071] 10 μL of COF@CB@MPDA dispersion droplets with a concentration of 1.5 mg / mL were coated on the surface of the bare glassy carbon electrode and dried at room temperature to obtain the modified electrode (COF@CB@MPDA / GCE).

[0072] 2. The saturated calomel electrode and the platinum electrode were used as the reference electrode and the auxiliary electrode respectively. The modified electrode COF@CB@MPDA / GCE in this experiment was used as the working electrode to form a three-electrode system. Differential pulse voltammetry was used in the potential window. Within the range of 0.7-1.3V, the working electrode was immersed in the standard solution of ciprofloxacin with concentrations of 0.5, 1, 5, 10, 20, 50 and 100 μmol / L, respectively, and the corresponding current value I was recorded respectively. P .

[0073]3. Draw the working curve with the concentration of ciprofloxacin standard solution as the abscissa and ...

Embodiment 2

[0077] Example 2: Specificity of the detection method of the present invention:

[0078] In order to explore the specific recognition ability of COF@CB@MPDA to ciprofloxacin, in this example, Enoxacin, Moxifloxacin and Enrofloxacin (Enoxacin), Moxifloxacin and Enrofloxacin ( Enrofloxacin) three structural analogs were tested for selectivity.

[0079] Figure 5 are the peak current values ​​of COF@CB@MPDA / GCE and COF@CB@NPDA / GCE in 50 mM ciprofloxacin and three structural analogs at equal concentrations; the synthesis method of COF@CB@NPDA is the same as that of COF@CB@ Compared with the synthesis of MPDA, the rest of the synthesis steps are the same except that ciprofloxacin is not added. The imprinting factors of COF@CB@MPDA / GCE for ciprofloxacin, enoxacin, moxifloxacin and enrofloxacin were calculated to be 1.98, 1.19, 0.76 and 1.10, respectively. It can be seen that COF@CB@MPDA / GCE only has good specific recognition ability for ciprofloxacin.

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Abstract

The invention discloses an electrochemical sensor for detecting ciprofloxacin and a detection method thereof, belonging to the technical field of food safety detection. The electrochemical sensor uses the electrode modified by the COF@CB@MPDA composite material as the working electrode; the COF@CB@MPDA composite material is prepared by the following method: disperse COF and CB in double distilled water to obtain a mixed solution; Then ciprofloxacin and dopamine are added into the mixed solution in a molar ratio of 1: (9-12), dispersed evenly, then added Tris-HCl (pH 8.5) solution, stirred at room temperature for 10-14h, and the resulting product was washed and After drying, the COF@CB@MPDA composite material is prepared. The minimum detection limit of ciprofloxacin by using the electrochemical sensor of the present invention is 9.46 μg / kg, which can meet the detection needs; the pretreatment and analysis time are respectively shortened by 48 minutes and 10 minutes compared with the traditional detection method, and it is suitable for ciprofloxacin Rapid detection of sand stars.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to an electrochemical sensor for detecting ciprofloxacin and a detection method thereof. Background technique [0002] Ciprofloxacin (CIP) is a synthetic third-generation fluoroquinolone antibacterial drug, and its structural formula is as follows: [0003] [0004] Ciprofloxacin has broad-spectrum antibacterial activity, and has good effects on both Gram-positive and Gram-negative bacteria. Therefore, ciprofloxacin is often used to treat digestive and respiratory infections and urinary tract infections. Ciprofloxacin can inhibit bacterial DNA helicase, prevent bacterial replication, and rapidly reduce bacterial reproduction. [0005] However, ciprofloxacin can sometimes cause allergic reactions, phlebitis, gastrointestinal reactions, and central nervous system reactions in humans. The Ministry of Agriculture of the People's Republic of China stipulates that the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/30Y02A50/30
Inventor 徐志祥孙玉奉陈永峰张鸿雁孔非凡
Owner SHANDONG AGRICULTURAL UNIVERSITY
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