Method for preparing water-soluble nutrient substances through mixed enzymolysis of earthworms and biomass
A technology of mixing enzymatic hydrolysis and nutrients, which is applied in the direction of fertilizer mixture, preparation of organic fertilizers, treatment of biological organic parts, etc., can solve the problems of not being fully developed and utilized, and achieve the effect of mild reaction conditions
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Embodiment 1
[0017] Example 1 Mixed enzymatic hydrolysis of earthworm and plant leaf protein to prepare polypeptide and amino acid mixture;
[0018] Step 1: Take fresh and live earthworms without pollution and place them in loose, moist and non-polluted mountain red soil, and place a sufficient number of peeled water chestnuts, apples, pears and other fruits with moderate sugar and acid content on them to promote their excretion of soil and feces in the cavity and intestines , washed with sterile water for later use;
[0019] Step 2: Take 200 kg of plant leaf protein powder, pulverize it with a hammer mill, pass it through a 40-mesh sieve, mix it with 50 kg of cleaned fresh earthworms in step 1, add 200 kg of tap water, and place in a In the stainless steel reaction tank of the cooling reflux device and the temperature rise temperature control device, the reaction temperature is set at 60°C ± 2°C, and the reaction is kept for 36 hours, during which the pH of the reactant is adjusted with d...
Embodiment 2
[0023] Example 2 Earthworms and animal hair are mixed and enzymatically hydrolyzed to prepare a mixture of polypeptides and amino acids;
[0024] Step 1: Take fresh and live earthworms without pollution and place them in loose, moist and non-polluted mountain red soil, and place a sufficient number of peeled water chestnuts, apples, pears and other fruits with moderate sugar and acid content on them to promote their excretion of soil and feces in the cavity and intestines , washed with sterile water for later use;
[0025] Step 2: Take 100 kg of air-cleaned animal hair, pulverize it with a hammer mill, pass through a 20-mesh sieve, mix it with 100 kg of cleaned fresh earthworms in step 1, add 150 kg of tap water, and place In a FRP reaction tank equipped with a cooling reflux device and a temperature control device, set the reaction temperature at 49°C ± 2°C, and keep it warm for 72 hours. During this period, adjust the pH of the reactant with dilute hydrochloric acid and dilu...
Embodiment 3
[0029] Example 3 Earthworms and animal tissues were mixed and enzymatically hydrolyzed to prepare polypeptide and amino acid mixtures;
[0030] Step 1: Take fresh and live earthworms without pollution and place them in loose, moist and non-polluted mountain red soil, and place a sufficient number of peeled water chestnuts, apples, pears and other fruits with moderate sugar and acid content on them to promote their excretion of soil and feces in the cavity and intestines , washed with sterile water for later use;
[0031] Step 2: Manually sort the internal organs of slaughterhouse animals and remove intestinal feces. After cleaning, beat them with a meat grinder. Take 150 kg of slurry, mix it with 150 kg of cleaned fresh earthworms in step 1, add 100 kg of tap water, placed in a ceramic reaction tank equipped with a cooling reflux device and a temperature rise temperature control device, set the reaction temperature at 55°C ± 2°C, and kept the temperature for 78 hours, during w...
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