Composite nanoparticle for photothermal and photodynamic synergistic therapy of tumors and preparation method of composite nanoparticle
A nanoparticle and photodynamic technology, which is applied in the fields of nanotechnology, antineoplastic drugs, and nanotechnology for materials and surface science, can solve the problems of multifunctional nano-preparation design, difficulty in designing small molecule inhibitors, etc., and achieve enhanced The effect of light heat and light power, simple and controllable preparation process, and simple process
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0060]This embodiment provides a method of preparing a pure metal nanoparticle comprising the steps of:
[0061]50 μl of a concentration of 20 mg / ml of rapamycin (RAP) ethanol solution and 50 μl of concentration of 20 mg / ml of dihydrogen E6 (CE6) DMSO solution was sequentially added to 5 ml of ultrapure water, ultrasonic stirring, centrifugal cleaning Pure drug nanoparticles.
Embodiment 2
[0063]This embodiment provides a method of preparing a composite nanoparticle, comprising the steps of:
[0064](1) 50 μl of concentration of 20 mg / ml of rapamycin (RAP) ethanol solution and 50 μl of concentration of 20 mg / ml of dihydrogen E6 (CE6) DMSO solution sequentially added to 5 ml of ultrapure water, ultrasonic stirring, centrifugation Cleaning is the pure drug nanoparticle RC.
[0065](2) The above pure drug nanoparticles (5 mL) were added to 2 ml of ultra-pure water, 2 mltris buffer (pH 8.5, 100 mm), 0.6 ml of dicamine (10 mg / ml), stirred for 48 hours, to obtain polypotransamine wrap pure drug nanometers Granular RC @ PDA.
[0066](3) 10 mL of nanoparticles (RC @ PDA), 2 ml of TRI Tris buffer (pH 8.5, 100 mM), 1 ml polyethylene glycol (10 mg / ml), stirred for 24 hours, centrifugal cleaning, the nanoparticle RC @ PP.
[0067](4) 2 ml of Tris buffer (pH 8.5, 100 mM), 0.6 ml of dopamine (10 mg / ml) was added to 2.6 ml of dopamine (10 mg / ml), and stirred for 48 hours to obtain a...
experiment example 1
[0069]The complex nanoparticles of Example 1 and Example 2 were performed as follows:
[0070]First, the particle size and potential: the particle size and potential of RC, RC @ PDA, RC @ PP, the measurement method is: Take the sample solution in the Marlven nano ZS instrument, using dynamic light laser scattering method to detect the particle size, measure the pool The temperature is set to 25 ° C, and 3 parts per sample are operated in parallel.figure 1 withfigure 2 For the particle size and potential variation of RC, RC @ PDA, RC @ PP, from the results of the figure, it is known that the RC particle diameter is 160 nm, the potential is -43mV, and the package polyammine (RC @ PDA) particle size increases to 190 nm, Potential rises to -22mV, continuing to wrap the polyethylene glycol to form the particle size of the complex nanoparticles RC @ PP to 210 nm, the potential is reduced to -9 mV.
[0071]Second, the morphology: observed the morphology of RC @ PP, the morphology detection metho...
PUM
| Property | Measurement | Unit |
|---|---|---|
| Particle size | aaaaa | aaaaa |
| Electric potential | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More