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Extraction method of saussurea involucrata flavonoid composition

An extraction method and a technology for flavonoids of snow lotus are applied in the field of extraction of flavonoids of snow lotus, which can solve problems such as difficulty in the research field, and achieve the effects of quick effect, high absorption rate and high pharmacodynamic activity.

Pending Publication Date: 2021-06-01
北京慧康天诚医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how its unique medicinal effect is produced and takes effect has always been a difficulty and blind spot in the research field

Method used

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  • Extraction method of saussurea involucrata flavonoid composition
  • Extraction method of saussurea involucrata flavonoid composition
  • Extraction method of saussurea involucrata flavonoid composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Extraction of flavonoids in Saussurea bractosa

[0027] 1.1 Alcoholic extraction of snow lotus medicinal materials

[0028] Take 6 snow lotus medicinal materials and weigh them before and after powdering as follows: No. 1: 28.5g, 28.3g; No. 2: 16.3g, 16.5g; No. 3: 13.3g, 13.5g; : 23.1g, 23.2g; No. 6: 14.6g, 14.7g. The total powder: 111.9g, 10 times the amount of 70% ethanol was heated and refluxed for 3 times, each time for 1h, filtered through 8 layers of gauze, and the extracts were combined.

[0029] 1.2 Preparation of sample solution

[0030] The solvent was recovered from the extract under reduced pressure to obtain about 70 mL of the sample solution.

[0031] 2 refinement

[0032] 2.1 Use AB-8 macroporous resin (soaked in 95% ethanol for 24 hours). The inner diameter of the column is about 2.5cm, and the ratio of diameter to height is 1:10. Soak the macroporous resin in absolute ethanol for 24 hours and then wet-pack the column. Before loading the c...

Embodiment 3

[0052] Components and compatibility of embodiment 3 snow lotus flavonoids composition

[0053] It can be obtained from the analysis and identification of Example 2 that the purified snow lotus obtained by the extraction method of the present invention mainly contains 4 kinds of flavonoids, which are respectively: rutin: myristicin: isoquercitrin: luteolin-7-o -glucoside, and the weight ratio between them is 30:1:2:7.

[0054] The drug efficacy test (analgesic effect) of the flavonoid composition of embodiment 4 embodiment 3

[0055] Objective: through the therapeutic effect of the flavonoid composition of embodiment 3 on acetic acid-induced mouse pain model, evaluate the pharmacodynamics of the flavonoid composition of embodiment 3.

[0056] Method: mice are divided into 5 groups at random, are respectively model control group (7), positive drug control group, the flavonoid composition high-dose group of embodiment 3, the flavonoid composition middle-dose group of embodiment ...

Embodiment 6

[0125] In order to further prove the effect of the Saussurea flavonoids composition of the present invention, on the basis of the compatibility of the components obtained in Example 3, in Example 6, four commercially available compounds were used to compound the composition of this application, and then the efficacy test was carried out. Test process is consistent with the test process of embodiment 4 and embodiment 5, repeats no more at this, compound ratio and test result are as shown in the following table:

[0126] Table 4 composition components and compound ratio (weight ratio)

[0127] components Rutin Myristicin isoquercitrin Luteolin-7-o-glucoside Compound ratio 30 1 2 7

[0128] Table 5 on the impact of acetic acid-induced mouse pain model

[0129]

[0130] Compared with the model control group: *P<0.05; **P<0.01

[0131] Table 6 Effects on Adjuvant Arthritis Mouse Model

[0132] group Dose (mg / kg) Number of animals (...

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Abstract

The invention relates to an extraction method of a saussurea involucrata flavonoid composition. The extraction method comprises the following steps: (1) alcohol extraction; (2) fine extraction; and (3) freeze-drying. According to the extraction method disclosed by the invention, the saussurea involucrata flavonoid composition containing four components, namely rutin, myristyl ether, isoquercitrin and luteolin-7-o-glucoside, can be obtained, research finds that the flavonoid composition has pharmacodynamic activity, can be used for preparing various clinically applied pain relieving and easing medicines and arthritis treatment medicines, and has the effects of high pharmacodynamic activity, small clinical dosage, quick effect, low side effect and the like compared with a Chinese patent medicine preparation prepared by directly applying saussurea involucrata.

Description

technical field [0001] The invention relates to the technical field of compositions for medical use, in particular to a method for extracting a Saussurea flavonoids composition and its use. Background technique [0002] Saussurea involucrata (scientific name: Saussurea involucrata (Kar. et Kir.) Sch.-Bip.), because its top is shaped like a lotus, it is named Saussurea involucrata, referred to as Saussurea involucrata. It is a perennial herb of the genus Asteraceae and Zephyr, 15-35 cm high. Rhizome thick, neck covered with many brown leaf remnants. Stem stout, glabrous. The leaves are dense, the basal leaves and stem leaves are sessile, and the leaves are elliptic or ovate-elliptic; the uppermost leaf bracts are leaf-like, membranous, light yellow, surrounding the total inflorescence, with sharp teeth on the edge. There are 10-20 inflorescence heads, densely forming a spherical total inflorescence at the top of the stem. Involucre hemispherical, 1 cm in diameter; involuc...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07C07D317/64A61P29/00A61P19/02A61P25/04
CPCC07H1/08C07H17/07C07D317/64A61P29/00A61P19/02A61P25/04
Inventor 徐宁万志超
Owner 北京慧康天诚医药科技有限公司
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