Preparation method of aspergillus oryzae soybean meal culture
A technology of aspergillus oryzae soybean meal and its production method, applied in animal feed, animal feed, application, etc., can solve problems such as insufficient performance, high cost of feed production, and animals cannot achieve production performance, so as to achieve good food attraction and improve intestinal health , Improve the effect of feeding value
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Embodiment 1
[0035] A kind of preparation method of aspergillus oryzae soybean meal culture, comprises the following steps:
[0036] (1) Multi-strain liquid mixed deep fermentation, select high-quality peeled soybean meal with protein content greater than 46%, add water according to the mass ratio of soybean meal to water 5:3, and steam sterilize at 115°C and 0.1MPa for 30 minutes in a cooking pot Then according to the mass ratio of sterilized soybean meal and Aspergillus oryzae spore powder 1000:1, Aspergillus oryzae spore content 20,000,000,000 cfu / g, above-mentioned each bacterial classification is inoculated on the soybean meal after the sterilization, adopts inoculation machine to inoculate, control The fermentation temperature is 32°C, the throwing speed is 40 rpm, the ventilation volume is 2.5L / (mim·square), the fan pressure is 0.01MPa, and the aerobic fermentation is carried out for 38h; then according to the mass ratio of soybean meal to Lactobacillus plantarum liquid of 200:1, the...
Embodiment 2
[0040] A kind of preparation method of aspergillus oryzae soybean meal culture, comprises the following steps:
[0041](1) Multi-strain liquid mixed deep fermentation: select high-quality peeled soybean meal with a protein content greater than 46%, add water according to the mass ratio of soybean meal to water 5:3, and steam sterilize at 115°C and 0.1MPa for 30 minutes in a cooking pot Then according to the mass ratio of sterilized soybean meal and Aspergillus oryzae spore powder 1000:1, Aspergillus oryzae spore content 20,000,000,000 cfu / g, above-mentioned each bacterial classification is inoculated on the soybean meal after the sterilization, adopts inoculation machine to inoculate, control The fermentation temperature is 32°C, the throwing speed is 40 rpm, the ventilation volume is 2.5L / (mim·square), the fan pressure is 0.01MPa, and the aerobic fermentation is carried out for 38h; then according to the mass ratio of soybean meal to Lactobacillus plantarum liquid of 200:1, th...
Embodiment 3
[0046] A kind of preparation method of aspergillus oryzae soybean meal culture, comprises the following steps:
[0047] (1) Raw material reception: raw material acceptance criteria, soybean meal / 46%: moisture, %≤13.0; crude protein, %≥46.0; protein solubility, % between 73.0 and 85.0; aflatoxin B1, ppb≤30; vomitoxin , ppb≤500; mold number, CFU / g ≤4×10 3 ;
[0048] (2) High-temperature sterilization: put 500Kg of soybean meal into a steamer with a natural pH value, adjust the water content to 42% to 44%, heat the steam to 118°C, and maintain it for 10 minutes;
[0049] (3) After the sterilized soybean meal is cooled to below 38°C through the natural air cooling system, it is transported to the distributing machine through the chain plate conveyor, and the inoculation machine is inserted into the Aspergillus oryzae strain (biomass 20 billion / g, The amount of addition is 0.04%, calculated on a dry basis) Online addition;
[0050] (4) Distributing: The distributing machine even...
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