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Preparation method and application of strain composition and medicated leaven

A composition and strain technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of large differences between product batches, difficult control of microorganism types and quantities, etc., to improve safety and Quality, good application prospects, and the effect of improving gastrointestinal dysfunction

Pending Publication Date: 2021-06-04
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The type and quantity of microorganisms in the natural fermentation process are not easy to control, resulting in large differences between product batches, and at the same time, miscellaneous bacteria will produce harmful metabolites, such as aflatoxin, etc.

Method used

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  • Preparation method and application of strain composition and medicated leaven
  • Preparation method and application of strain composition and medicated leaven
  • Preparation method and application of strain composition and medicated leaven

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] (1) Prepare the plate:

[0076] Medium I: Take yeast extract powder peptone glucose agar medium (1g peptone, 0.5g yeast extract powder, 2g glucose, 1.4g agar) 4.9g and add deionized water to make 100mL;

[0077] Medium II: Take 3.3 g of nutrient agar (1 g of peptone, 0.5 g of sodium chloride, 0.3 g of beef extract powder, and 1.5 g of agar) and add deionized water to make 100 mL.

[0078] The above two mediums were sterilized by high pressure steam (100kPa, 121°C, 20min). The sterilized culture medium is poured into a sterile flat petri dish while it is hot in an ultra-clean bench, sealed and cooled, and set aside.

[0079] (2) The two plate cultures prepared by medium I and medium II were respectively inoculated with Saccharomycopsis fibuligera, the preservation number of which was CICC No.33177; Bacillus subtilis subsp.Subtilis ), the deposit number is CICC No.24341. After inoculation, they were cultured at 30°C and 75% RH for 24 hours.

[0080] (3) Liquid bacteri...

Embodiment 2

[0091] The only difference with embodiment 1 is:

[0092] In step (6), the bacterial liquid is inoculated according to the inoculum amount of 0.5mL / g (the ratio of the bacterial liquid volume to the matrix mass, unit mL / g), and the bacterial liquid of Bacillus subtilis: the volume of the bacterial liquid of Saccharomyces peritonei The ratio = 4:1 is sprinkled on the sterilized substrate, sealed with a parafilm, and placed in a constant temperature and humidity chamber at 33°C and 75% RH for 5 days.

Embodiment 3

[0094] The only difference with embodiment 1 is:

[0095] In step (6), the bacterial liquid is inoculated according to the inoculum amount of 0.2mL / g (the ratio of the bacterial liquid volume to the matrix mass, unit mL / g), and the bacterial liquid of Bacillus subtilis: the volume of the bacterial liquid of Saccharomyces peritonei The ratio = 4:1 is sprinkled on the sterilized substrate, sealed with a parafilm, and placed in a constant temperature and humidity chamber at 33°C and 75% RH for 5 days.

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Abstract

The invention discloses a strain composition and application thereof as well as a preparation method and application of medicated leaven. The strain composition comprises peritoneum fibuligerum and bacillus subtilis. The strain composition is safe and harmless, has protease and amylase production activity, can be symbiotic and can improve the enzyme activity of medicated leaven, and compared with existing aspergillus fungi used for synergistic fermentation, the yeast fungi avoid production of mycotoxin and are safer.

Description

technical field [0001] The application relates to a strain composition and its application, a preparation method of Shenqu and its application, and belongs to the technical field of biological products. Background technique [0002] Six Divine Comedy (massa medicata fermentata, MMF), also known as Divine Comedy and Liuqu, was first used as medicine in the "On the Nature of Medicine" written by Zhen Quan in the Tang Dynasty: "Hua Shui Gu Su Shi, the crux of the disease stagnates, invigorates the spleen and warms the stomach." Now it is mostly fermented from flour, wheat bran, red bean, Artemisia annua, Cocklebur, Polygonum spicosa, and bitter almond in a certain proportion. effect. Shenqu is a commonly used traditional Chinese medicine in traditional Chinese medicine preparations and has a wide range of clinical applications. The 2015 edition of the "Chinese Pharmacopoeia" contains 41 prescriptions for the treatment of food accumulation (gastrointestinal diseases) with Shen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20C12P1/02C12P1/04A23L29/00C12R1/645C12R1/125
CPCC12N1/16C12N1/20C12P1/02C12P1/04A23L29/065A23V2002/00A23V2250/76
Inventor 詹雪艳陈瑾王舒玉吴春颖
Owner BEIJING UNIV OF CHINESE MEDICINE
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