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Production method of turtle peptide

A production method and technology for tortoise meat, applied in the field of biological preparation, can solve problems such as the inability to reflect the components of whole tortoises and the limited components of tortoise peptide products, and achieve the effects of high production efficiency, regulating blood lipids and improving sleep.

Pending Publication Date: 2021-06-11
海南金龟岛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the methods for preparing tortoise peptides from turtle organisms all use a single species of tortoise as raw materials, and either live tortoise tissues or tortoise shell glue are used as raw materials to prepare tortoise peptides by enzymatic hydrolysis, but tortoise peptide products The ingredients are limited and cannot reflect the whole turtle ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Take the fresh adult tortoises of the golden tortoise, stone tortoise, and Chinese tortoise. After slaughtering, cleaning, and deboning, the turtle meat is mixed according to the weight ratio of 1:1:1, and then mechanically crushed and ultrasonically crushed. The pulp of turtle meat, the solid content of which is no more than 10 mesh.

[0028] (2) According to the method described in the patent CN104946488A, tortoise shells are taken to prepare the tortoise shell extract.

[0029] (3) Take 1000 g of tortoise minced pulp and 100 g of tortoise shell extract, mix and homogenize, and then prepare a 10% (W / V) substrate solution with ultrapure water.

[0030] (4) Add 0.5 mg / mL papain to the substrate solution, perform enzymatic hydrolysis at a constant temperature of 65°C for 15 hours, and adjust the pH to 6.5±0.1 during this period.

[0031] (5) After deactivating and sterilizing the obtained enzymolysis products, they are sequentially filtered with ultrafiltration memb...

Embodiment 2

[0035] (1) Take the fresh adult turtles of money turtle, crocodile turtle, yellow-bordered box turtle, flat-breasted turtle, and box turtle. After the meat is mixed, it is mechanically crushed and ultrasonically crushed into a pulp of turtle meat, and the solid matter in it does not exceed 10 mesh.

[0036] (2) According to the method described in the patent CN104946488A, tortoise shells are taken to prepare the tortoise shell extract.

[0037] (3) After mixing and homogenizing 2000 g of tortoise meat slurry and 400 g of tortoise shell extract, a 20% (W / V) substrate solution was prepared with ultrapure water.

[0038] (4) Add 0.8 mg / mL complex protease to the substrate solution, and perform enzymatic hydrolysis at a constant temperature of 55°C for 20 hours, during which the pH is adjusted to 5.5±0.1.

[0039] (5) After deactivating and sterilizing the obtained enzymolysis products, they are sequentially filtered with ultrafiltration membranes with molecular weight cut-offs o...

Embodiment 3

[0043] (1) Take the fresh adult turtles of stone money turtle, Chinese tortoise, crocodile turtle, and yellow edge box turtle, after slaughtering, cleaning, and deboning, mix the turtle meat according to the weight ratio of 1:1:1:1: Respectively through mechanical crushing and ultrasonic crushing to form turtle meat slurry, the solid matter in which does not exceed 10 meshes.

[0044] (2) According to the method described in the patent CN104946488A, tortoise shells are taken to prepare the tortoise shell extract.

[0045] (3) After mixing and homogenizing 3000 g of tortoise meat slurry and 1200 g of tortoise shell extract, a 35% (W / V) substrate solution was prepared with ultrapure water.

[0046] (4) Add 0.8 mg / mL complex protease to the substrate solution, and perform enzymatic hydrolysis at a constant temperature of 50°C for 18 hours, during which the pH is adjusted to 7.0±0.1.

[0047] (5) After deactivating and sterilizing the obtained enzymolysis products, they are seque...

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Abstract

The invention belongs to the technical field of biological preparation, and relates to a production method of turtle peptide, which comprises the following steps: slaughtering adult live turtles, cleaning, removing bones, and crushing into turtle meat paste slurry; mixing the tortoise shell extract and the tortoise meat paste slurry, performing ultrasonic homogenization, blending with ultrapure water to obtain a substrate solution, and adding protease for enzymolysis; and carrying out enzyme deactivation, sterilization, ultrafiltration separation and vacuum freeze drying on the reaction product obtained by enzymolysis to obtain the soluble powdery turtle peptide. According to the turtle peptide produced by taking multiple turtle compound components as raw materials and applying an enzymolysis method, active peptide molecules in adult turtles are extracted to the maximum extent, meanwhile, other bioactive components in the raw materials are preserved, and multiple bioactive components supplement one another. The turtle peptide can effectively regulate immunity, resist oxidation, resist tumors, resist fatigue, regulate blood fat, improve sleep and delay senescence, so that the effects of nourishing yin, tonifying yang, building bodies, maintaining beauty, keeping young and prolonging life are achieved.

Description

technical field [0001] The invention belongs to the technical field of biological preparation, and relates to a production method of tortoise peptide, in particular to a production method of tortoise peptide with multiple sources of tortoise species and relatively evenly distributed amino acid polymerization degree. Background technique [0002] Modern scientific research has found that the long life of turtles, which have the meaning of "longevity and auspiciousness" in traditional Chinese culture, is not only due to their unique physiological structure and living habits, but also because they are rich in immune-regulating, anti-oxidant, anti-tumor , Anti-fatigue, regulating blood lipids, improving sleep, anti-aging and other functions of bioactive substances, such as unsaturated fatty acids, vitamin E, selenium and other trace elements, active protein, collagen, free fatty acids, etc., so that it can survive cancer, heart disease, etc. Cerebrovascular disease, diabetes and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC12P21/06C07K1/34C07K1/14
Inventor 张世凯
Owner 海南金龟岛生物科技有限公司
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