Method for concentrating DHA (docosahexaenoic acid) in schizochytrium limacinum grease
A technology of Schizochytrium and oil, which is applied in the field of lipase hydrolysis and concentration of DHA in Schizochytrium oil, can solve the problems of using a large amount of organic solvents, unfriendly environment, low selectivity, etc., achieve low price, avoid DHA Stable and selective effect
Active Publication Date: 2021-06-18
ZHEJIANG NORMAL UNIVERSITY
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Problems solved by technology
[0006] The source of Schizochytrium oil is obtained from the fermentation and separation of Schizochytrium. At present, the DHA concentration process for Schizochytrium oil is low-temperature crystallization method and urea inclusion method. The defect of this process is that the selectivity is low , both require a large amount of organic solvents, which are not environmentally friendly
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Embodiment 1
[0050] Embodiment 1, AO lipase 12h hydrolyzes Schizochytrium fat, carries out the following steps successively:
[0051] Step 1), add Schizochytrium oil, 0.1mol / L pH 7.0 phosphate buffer solution, and AO lipase into the stirred tank reactor, mix all materials evenly, fill with nitrogen gas, seal and place at 40°C, stir The speed is 140r / min, and the reaction is 12 hours;
[0052] The amount of AO lipase added is 2% of the mass of Schizochytrium oil; the ratio of material to liquid of Schizochytrium oil and phosphate buffer solution is 1g / 1ml;
[0053] Step 2), after the enzymolysis time is up, place the reaction solution obtained in step 1) in a boiling water bath for 10 minutes to inactivate the enzyme, thereby ending the reaction, then cool to room temperature, and centrifuge at 4°C and 5000rpm for 10min (accelerated fractionation). phase), collecting the supernatant;
[0054] Step 3), the separation of lipid:
[0055] The supernatant collected in step 2) was used to sepa...
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The invention discloses a method for concentrating DHA (docosahexaenoic acid) in schizochytrium limacinum grease. The method comprises the following steps: stirring the schizochytrium limacinum grease, a phosphoric acid buffer solution and AO lipase at 35-45 DEG C for enzymolysis reaction for 8-16 hours under the protection of inert gas; separating lipid from the upper-phase clear liquid obtained by the reaction through preparative-grade thin-layer chromatography, and collecting each developing band after the chromatography is finished; and performing ultrasonic extraction on each developing band by using dichloromethane, and performing nitrogen blowing to remove the solvent, so as to obtain monoglyceride containing DHA, diglyceride containing DHA and triglyceride containing DHA. The method is mild in operation condition, high in selectivity, green and environmentally friendly, rapid in process and low in price, the DHA enrichment efficiency is improved to a certain extent, and instability of the obtained DHA is effectively avoided.
Description
technical field [0001] The invention relates to a method for hydrolyzing and concentrating DHA in Schizochytrium oil with lipase. Background technique [0002] Docosahexaenoic Acid (DHA), the most common Omega-3 polyunsaturated fatty acid (Polyunsaturated fatty acids, PUFAs), is generally believed to play an important role in the development of human intelligence and optic nerve It plays an important role and is widely used in dairy products, nutritional and health products and other fields. In recent years, the market size of DHA in my country has continued to expand, and the total market value has reached about 36 billion yuan. At present, the main sources of DHA are fish and microalgae. The DHA content in fish oil is relatively low, only about 10%, while the DHA content in microalgae lipids varies with algae species and culture methods, and the highest is about 40%. Because the above-mentioned raw materials often contain impurities such as pigments and other saturated fat...
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IPC IPC(8): C12P7/64
CPCC12P7/6472C12P7/6454
Inventor 邓刚王芳周雪华傅新媛吴晓敏
Owner ZHEJIANG NORMAL UNIVERSITY