Bacillus laterosporus and application thereof in nematode disease prevention and treatment
A technology of Bacillus lateralis and Bacillus, which is applied to Bacillus lateralis and its application in agricultural production, and can solve problems such as few biological control preparations
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Embodiment 1
[0026] Isolation and screening of embodiment 1 bacterial strain
[0027] 1. Sample source: Tomato rhizosphere soil in Pingdu Vegetable Greenhouse, Qingdao City, Shandong Province.
[0028] 2. Strain isolation:
[0029] Weigh 10 g of soil sample into a 250 mL Erlenmeyer flask with sterile glass beads and 90 mL of sterile water, and shake well. After standing still, use a pipette gun to take 1ml of the supernatant and add it to 9ml of sterile water, and carry out gradient dilution with sterile water to obtain 10 -4 、10 -5 、10 -6 Take 100 μL of each of the three gradient dilutions and spread them on the nutrient agar plate, and repeat 3 times. Afterwards, culture them in a 37°C incubator, observe the growth after 24 hours, classify them according to the characteristics of colony size, shape and color, and pick a single colony for purification and culture. Finally, 5 strains were selected and named as B1, B2, B3, B4, B5, respectively, and kept in liquid glycerin.
[0030] 3....
Embodiment 2
[0036] Identification of embodiment 2 bacterial strain ZB387
[0037] 2.1 Identification of colony morphology
[0038] Such as figure 1 As shown, the colonies of the ZB387 strain on the nutrient agar medium were round, with irregular edges and smooth surfaces, and the color of the colonies was white.
[0039] 2.2 Molecular identification of 16S rRNA
[0040]The genome of strain ZB387 was extracted using a kit. Then using the genome as a template, the 16S rRNA was amplified using specific primers. The amplified PCR product was detected by 1% agarose gel electrophoresis, and the result showed that the size of the PCR product was about 1500bp, which met the requirements. The PCR amplification product was sent to a sequencing company for sequencing.
[0041] Sequencing results showed that the sequence of the PCR amplification product was SEQ ID NO:1. The sequence was compared with BLAST in the NCBI database, and the sequence had the highest similarity with Bacillus laterospo...
Embodiment 3
[0046] Example 3 Evaluation of the Enzyme Production Ability of Bacillus Laterosporus ZB387
[0047] The applicant evaluated the enzyme-producing ability of Bacillus lateralis ZB387 by the transparent circle method. Inoculate Bacillus lateralis ZB387 on the plate with the corresponding substrate, and the enzyme produced during the growth of the strain will decompose the corresponding substrate, forming an observable enzyme-producing circle. The size of the enzyme-producing circle represents the strength of the enzyme-producing ability of the bacteria.
[0048] Bacillus lateralis ZB387 was streaked on a nutrient agar medium plate for activation, and the corresponding substrate medium was sterilized and then poured into the plate, and placed upside down in a 37°C incubator for overnight culture. Use a sterilized toothpick to inoculate the activated strain on each medium, mark it, and place it in a 37°C incubator for 3 to 5 days. Use a ruler to measure the diameter of the enzym...
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