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Double-electrode photoelectrochemical aptamer sensor as well as preparation method and application thereof

An aptamer sensor and photoelectrochemical technology, applied in the field of materials, can solve problems such as low sensitivity, biological threats, and limited instruments, and achieve the effects of improving self-power supply capability, wide light absorption range, and increasing transmission rate

Active Publication Date: 2021-06-29
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the abuse of OTC drugs, their residues in the environment may accumulate and transfer in the ecological cycle, leading to antibiotic resistance and posing a threat to organisms
So far, the traditional analytical techniques are mainly high performance liquid chromatography, colorimetry, electrochemiluminescence and fluorescence, but they are limited by expensive and complicated instruments, time-consuming sample preparation process, or large errors , low sensitivity, etc.

Method used

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  • Double-electrode photoelectrochemical aptamer sensor as well as preparation method and application thereof
  • Double-electrode photoelectrochemical aptamer sensor as well as preparation method and application thereof
  • Double-electrode photoelectrochemical aptamer sensor as well as preparation method and application thereof

Examples

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preparation example Construction

[0058] A method for preparing a dual-electrode photoelectrochemical aptasensor, comprising the following steps: coating a suspension of reduced graphene oxide loaded with gold nanoparticles on the surface of a reaction end of a second conductive glass electrode to obtain a reduction of the loaded gold nanoparticles. The second conductive glass electrode modified by graphene oxide; the specific aptamer probe solution is added dropwise to the reaction end surface of the second conductive glass electrode modified by reduced graphene oxide loaded with gold nanoparticles, and added to 6-mercaptoethanol Cultured in the solution, the specific aptamer probe was immobilized on the surface of the reaction end of the second conductive glass electrode through the covalent bond of gold sulfhydryl, and the specific aptamer probe and the reduced graphene oxide modified with gold nanoparticles were obtained. The second conductive glass electrode; the ternary composite material suspension of gr...

Embodiment 1

[0065] A dual-electrode photoelectrochemical aptasensor, comprising a first conductive glass electrode used as a working electrode and a second conductive glass electrode used as a counter electrode in a dual-electrode system, the reaction end surface of the first conductive glass electrode is modified with a graphite phase The ternary composite material of carbon nitride / tungsten oxide / manganese dioxide, the ternary composite material of graphite phase carbon nitride / tungsten oxide / manganese dioxide is based on graphite phase carbon nitride nanosheets, and graphite phase carbon nitride The nanosheets are loaded with tungsten oxide nanoparticles and manganese dioxide nanosheets; the reaction end surface of the second conductive glass electrode is modified with a composite film, and the composite film is composed of reduced graphene oxide loaded with gold nanoparticles; the reduction and oxidation of gold nanoparticles loaded Graphene uses reduced graphene oxide as a carrier, an...

Embodiment 2

[0090] A two-electrode photoelectrochemical aptabody sensor, which is basically the same as the two-electrode photoelectrochemical aptabody sensor in Example 1, the only difference being: the specific aptamer detection in the two-electrode photoelectrochemical aptabody sensor in Example 2 The needle is an oxytetracycline-specific aptamer probe, wherein the oxytetracycline-specific aptamer probe has the nucleotide sequence shown in SEQ ID No.1, specifically: 5'-(SH)-(CH2 ) 6-GGAATTCGCTAGCACGTTGACGCTGGTGCCCGGTTGTGGTGCGAGTGTTGTGTGGATCCGAGCTCCACGTG-3'.

[0091]A preparation method of the above-mentioned two-electrode photoelectrochemical aptasensor of this embodiment is basically the same as the preparation method of Example 1, the only difference being: in the preparation method of Example 2, the specificity used in step (5) The aptamer probe solution is oxytetracycline specific aptamer probe solution.

[0092] For the dual-electrode photoelectrochemical aptasensor prepared in E...

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Abstract

The invention discloses a double-electrode photoelectrochemical aptamer sensor as well as a preparation method and application thereof. The sensor comprises a first conductive glass electrode modified by a ternary composite material and used as a working electrode, a specific aptamer probe used as a counter electrode, and a second conductive glass electrode modified by reduced graphene oxide loaded with gold nanoparticles. The preparation method comprises the steps of preparing the working electrode and the counter electrode. The dual-electrode photoelectrochemical aptamer sensor has the advantages of high stability, long service life, strong anti-interference capability, high detection sensitivity, wide detection range, low detection limit and the like, can realize specific detection of pollutants (such as antibiotics) in media such as water bodies and organisms, and is high in utilization rate, high in use value and good in application prospect; and meanwhile, the preparation method has the advantages of simple process, convenience in operation, safety, low cost, no pollution, high preparation efficiency and the like, is suitable for large-scale preparation and is beneficial to industrial application.

Description

technical field [0001] The invention belongs to the field of material technology, and relates to a dual-electrode photoelectrochemical aptamer sensor and a preparation method and application thereof. Background technique [0002] As an emerging sensing technology, the self-powered photoelectrochemical sensor (PEC) not only has the advantages of simple structure, fast response, high sensitivity and low cost of the traditional PEC sensing system, but also can be measured without external voltage. Reducing voltage disturbances has attracted widespread attention. In the past few years, self-powered PEC sensors were mainly based on a three-electrode system with a single working electrode, which uses a single working electrode as a signal source and a specific recognition platform at the same time, due to the interaction between the photoelectrode and the reducing substance or target molecule. Interaction inevitably leads to performance degradation. [0003] To overcome the shor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/36G01N27/26C01B21/06C01B32/19C01B32/194C01G41/02C01G45/02
CPCG01N27/3278G01N27/36G01N27/26C01B32/19C01B32/194C01B21/0605C01G41/02C01G45/02
Inventor 汤琳张紫翎彭博欧阳细莲朱旭范欣雅
Owner HUNAN UNIV
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