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DCM solution relative fluorescence emission intensity standard substance and its preparation method and application

A technology of emission intensity and standard substance, applied in fluorescence/phosphorescence, material excitation analysis, material analysis through optical means, etc., can solve problems such as large uncertainty, low luminous intensity, and inappropriateness

Active Publication Date: 2022-01-04
广东省计量科学研究院(华南国家计量测试中心)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic fluorescent standard substance is mainly GBW(E)130100 quinine sulfate fluorescent standard substance, and its certificate provides the purity and its uncertainty, the magnitude and uncertainty of fluorescence emission intensity at different emission wavelengths, and its maximum excitation The wavelength is 350nm, the maximum emission wavelength is 450nm, but below 395nm or above 565nm, the standard value of the luminous intensity is small (<0.1), the uncertainty in this range is large, and the wavelength interval of the standard value is as long as 5nm, which is not suitable for the requirements of some sophisticated fluorescence spectrometers

Method used

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  • DCM solution relative fluorescence emission intensity standard substance and its preparation method and application
  • DCM solution relative fluorescence emission intensity standard substance and its preparation method and application
  • DCM solution relative fluorescence emission intensity standard substance and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1D

[0071] The development of embodiment 1DCM n-propanol solution relative fluorescence emission intensity standard substance

[0072] 1. Standard material sample preparation

[0073] 1.1 Raw materials and reagents

[0074] Raw materials: Commercially purchased high-purity 4-(dicyanomethylene)-2-methyl-6-(4-dimethylaminostyryl)-4H-pyran, purchased from Bailingwei Technology (J&K) Co., Ltd. , 1 bottle.

[0075] Solvent: chromatographically pure n-propanol (CAS: 71-23-8), purchased from Fisher Co., Ltd., in a 4 L package.

[0076] 1.2 Equipment

[0077] 1.2.1 Equipment for standard substance preparation: 2000mL grade A volumetric flask; balance with accuracy of 1 / 100,000 (Sartorius, Germany); electric pipette (Eppendorf, Germany); class 100 clean bench, etc. Passed the test.

[0078] 1.2.2 Standard substance packaging equipment: 5mL brown ampoule bottle and ampoule melting and sealing machine and other equipment.

[0079] 1.2.3 Equipment for standard substance analysis:

[00...

Embodiment 2

[0282] The use result of embodiment 2DCM n-propanol solution relative fluorescence emission intensity standard substance for different instrument parts

[0283] Referring to the standard material development process of NIST’s SRM2944, fluorescent standard materials should also be considered when using ① the position of the incident excitation light irradiated on the sample changes, resulting in uncertainty introduced by changes in absorption and inner filter effects; ② the incident and exit grating slits Uncertainty introduced; ③Uncertainty introduced by instrument components such as light sources, reflectors, and detectors; ④Uncertainty introduced due to light anisotropy and polarization of standard substances.

[0284] 1.1 The position change of incident light and the influence of light anisotropy

[0285] When examining the first and fourth factors, since the DCM n-propanol solution is uniform relative to the fluorescence emission intensity standard substance solution syste...

Embodiment 3

[0294] Example 3 Similar Standard Substance Quantity Comparison Verification

[0295] Use respectively:

[0296] 1. The SRM2940 standard substance of NIST (the selection reason is because the scope of its emission wavelength is basically consistent with the relative fluorescence emission intensity standard substance of DCM n-propanol solution, and the size is the same during measurement) and the relative fluorescence of the DCM n-propanol solution prepared in Example 1 Emission intensity standard substances were compared for analysis.

[0297] 2. GBW (E) 130100 quinine sulfate fluorescent standard substance (the selection reason is because the scope of its emission wavelength overlaps with three kinds of relative fluorescence emission intensity standard substances, and is all in liquid form) and the DCM n-propylene oxide prepared in Example 1 The alcohol solution was compared with the fluorescent emission intensity standard substance.

[0298]Calibrate two fluorescence spect...

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Abstract

The invention discloses a DCM solution relative fluorescence emission intensity standard substance, a preparation method and an application thereof. The DCM solution relative fluorescence emission intensity standard substance provided by the present invention can be provided under the excitation of 430-500nm, the emission wavelength coverage range is 450-830nm, and the wavelength interval is the standard relative fluorescence emission intensity value of 1nm, which can well meet the requirements of related instruments. Calibration requirements for optical and near-infrared bands. The DCM solution relative fluorescence emission intensity reference substance provided by the present invention is mainly used as the standard of value transmission, meets the needs of current research and detection, and can be used for fluorescence instruments (such as spectrometer, spectrophotometer, microplate reader, gene amplification instrument, Calibration of detectors and detection modules, evaluation of analytical methods, and quality control in biology, food, pharmaceuticals, chemical products, and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of molecular fluorescence analysis and measurement, and in particular relates to a DCM solution relative fluorescence emission intensity standard substance and its preparation method and application. Background technique [0002] Fluorescence spectrometer / fluorescence spectrophotometer is an important and effective analytical instrument in spectral analysis. Fluorescence detection has the advantages of high sensitivity, strong selectivity, small sample size, non-destructive testing and simple method. Spectral analysis is not only an important subject in basic research, but also has many applications in fields such as industry, agriculture, medicine, environmental protection, commodity inspection, security and national defense. In recent years, new technologies, new devices, and new processes of fluorescence spectroscopy have emerged continuously, and their applications have become more and more extensive. M...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417
Inventor 黄彦捷罗思婷周瑾艳陈玲尹强廖泽荣
Owner 广东省计量科学研究院(华南国家计量测试中心)
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