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Photosynthetic reactor fermentation tube for culturing microalgae

A technology for photosynthetic reactors and fermentation tubes, which is applied to specific-purpose bioreactors/fermenters, combinations of bioreactors/fermenters, photobioreactors, etc. To improve the fermentation efficiency and improve the discharge efficiency

Inactive Publication Date: 2021-07-13
南京福喆未来食品研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main body of the existing fermenter has a slow fermentation efficiency during the use process, and is prone to poor quality of microalgae, low utilization rate, etc., and the structure is complex and the equipment cost is high

Method used

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  • Photosynthetic reactor fermentation tube for culturing microalgae

Examples

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Comparison scheme
Effect test

Embodiment 1

[0022] A photosynthetic reactor fermentation tube for cultivating microalgae, such as figure 1 As shown, it includes a fermenter main body 1, an LED lamp 2, a pH sensor 3, a temperature sensor 4, a gas concentration sensor 5, a temperature regulating plate 6, an air compressor 11, a gas cylinder 12 and a main control machine 13. The fermenter The main body 1 is used to hold photosynthetic microalgae. The inner wall of the fermenter main body 1 is provided with an LED light 2, and the microalgae in the fermenter main body 1 can receive the light emitted by the LED light 2. The inner wall of the main body 1 of the fermenter is designed to be opaque. The shape of the main body 1 of the fermenter is preferably cylindrical, and its size can be: 50 cm high, 24 cm in diameter, and 20 L in volume. It only needs to satisfy the technical effects to be achieved by the embodiments of the present invention. The LED lamp 2 has a complete waterproof function and can work normally in liquid ...

Embodiment 2

[0036] In this example, the photosynthetic reactor fermenter proposed in Example 1 is used to cultivate Spirulina. Spirulina is a kind of algae with fast growth rate, strong adversity tolerance and high content of functional substances. It is commonly used in the study of biological carbon fixation. Algal species, the spirulina of the present embodiment selects Spirulina dunnii (Spirulina dunnii), takes Spirulina dunnii as an example, optimizes the culture conditions, improves the output of phycobiliprotein; cultivating Spirulina specifically includes the following steps:

[0037] Step 1: Temperature Optimization

[0038] The Spirulina seed solution was inoculated in the main body of the fermenter, and the initial cell density was controlled at 1.2×10 6 In the range of / mL, the pH is controlled within the range of about 8, air is introduced, and the DO value (dissolved oxygen) is controlled within the range of 30% to 50%, with 5°C as the interval, set 20°C, 25°C, 30°C, The te...

Embodiment 3

[0046] In this embodiment, the photosynthetic reactor fermenter proposed in Example 1 is used in this embodiment to cultivate Nitzschia laevis. The Nitzschia laevis in this embodiment is selected from UTEX 2047, Austin, Texas, University of Texas Algae Culture library, the medium used is LDM medium, and the composition ratio is 1g tryptone, 892mL artificial seawater, 100mL Bristol solution, 6mL PIV metal solution, 1mL biotin stock solution (25.0×10 -5 g / L) and 1mL vitamin B12 (15.0×10 -5 g / L), all fermentation media were added with 120mg / L Na 2 SiO 3 9H 2 O, 0.68g / L NaNO 3 And 5g / L glucose, cultivating Nitzkiri specifically comprises the following steps:

[0047] S1. Adjust the medium to pH 8.5 and autoclave at 121°C for 20 minutes. The cell suspension was centrifuged at 3000 g for 5 min, and the cell pellet was washed twice with distilled water before being transferred to the main body of the fermenter, which contained fresh LDM medium. Add pre-sterilized glucose stock ...

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Abstract

The invention discloses a photosynthetic reactor fermentation tube for culturing microalgae, and belongs to the technical field of microalgae culture. The photosynthetic reactor fermentation tube comprises a fermentation tank main body; an LED lamp is arranged on the inner wall of the fermentation tank main body, a pH sensor, a temperature sensor and a gas concentration sensor are arranged in the fermentation tank main body, a gas inlet, a feeding hole and a gas exhaust hole are formed in the top of the fermentation tank main body, the gas inlet is communicated with an air compressor and a gas cylinder, the gas exhaust hole is communicated with a gas exhaust port, and the feeding hole is connected with a feeding port; a liquid outlet is formed in the side wall of the fermentation tank main body; the outer side of the fermentation tank main body is wrapped with a temperature regulator. The tube can culture and screen excellent algae species of target products by controlling single factor variables under the conditions of temperature, pH value, gas concentration, different nutrient substances and the like, and is high in fermentation efficiency, high in discharging effect, simple in structure and convenient to use.

Description

technical field [0001] The invention relates to a photosynthetic reactor fermentation tube for cultivating microalgae, belonging to the technical field of microalgae cultivation. Background technique [0002] With the intensification of global environmental pollution, continuous climate change, population growth and resource depletion, how to ensure safe, nutritious and sustainable food supply is facing great challenges. These challenges place new demands on how and how food will be delivered in the future. The use of cell factory manufacturing to replace traditional food acquisition methods and the establishment of a new sustainable food manufacturing model will greatly reduce the demand for resources and energy in food production, reduce greenhouse gas emissions, and improve the controllability of food production and manufacturing, effectively avoiding the Potential food safety and health risks. Microalgae, one of the untapped resources, are considered as promising and p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12M1/02C12M1/00
CPCC12M21/02C12M23/48C12M27/02C12M29/06C12M29/20C12M31/10C12M41/12C12M41/22C12M41/26C12M41/32
Inventor 徐勇将刘元法尹世鹏
Owner 南京福喆未来食品研究院有限公司
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