Preparation and application of biosensor based on polylactic acid nanowires
A technology of biosensor and polylactic acid, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of biosensor and large size of optical waveguide that have not been reported yet, and achieve the effect of easy operation, high sensitivity and low loss
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Embodiment 1
[0033] A preparation method of a biosensor based on polylactic acid nanowires:
[0034] Preparation of polylactic acid solution: Firstly, commercially available polylactic acid solids were divided into small pieces for subsequent dissolution; polylactic acid was ultrasonically cleaned with deionized water and alcohol (concentration 75% to 95%) at room temperature for 15mins, and then placed Blow dry under high pressure nitrogen flow. Weigh 4g of clean polylactic acid solids and put them into a conical flask, then add 30mL of organic solvent, put a stirrer in the bottle, place the conical flask in a magnetic stirrer at room temperature and stir for 24h at a speed of 1000rpm to obtain A solution of polylactic acid at a concentration of 0.13 g / mL.
[0035] Preparation of light cone: Use a fiber knife to strip one end of the single-mode silica fiber (interface: DC / PC, inner diameter: 9 μm, cladding: 125 μm) to expose the fiber core. Suspend the fiber core in the outer flame of a...
experiment example 2
[0038] The application of biosensor in cytochrome c (cytochrome c, cyt c) detection comprises the following steps:
[0039] S1. Place the polylactic acid nanowire biosensor with a diameter of 400nm and a sensing length of 100 μm in a micron groove, connect the other end of the polylactic acid nanowire to an optical fiber taper, and support the two ends of the polylactic acid nanowire with a magnesium fluoride substrate;
[0040] S2. Fill the cyt c sample solution into the micrometer groove, and incident the light of 532nm from one end of the fiber cone, measure the output intensity of the light in the other end of the light cone, and calculate the attenuation intensity of the light;
[0041] S3. Take 10 -18 to 10 -5 Draw a standard curve with the standard concentration of M and the corresponding attenuation intensity, and quantify the biological samples to be tested according to the standard curve.
[0042] Such as figure 2 As shown in a, when light propagates in a nanowir...
Embodiment 3
[0046] Based on the high-sensitivity sensing properties of PLA nanowires to cyt c, PLA nanowires are further used for real-time monitoring of biological cell apoptosis, such as image 3 shown. The cells used were Cyt C-rich yeast cells, and the cells were dispersed in an aqueous solution with a cell concentration of 1.96 × 10 3 piece / mm 2 . Concentration of 0.8 wt% H 2 o 2 Inducer of apoptosis. The above-mentioned PLA nanowires with a diameter of 400 nm and a sensing length of 100 μm were used as sensor devices. Figure 3b is the optical microscope image of the PLA biosensor in the cell solution at T=0, that is, just adding H 2 o 2 . The direction of the red arrow indicates the direction of light propagation. 40 minutes later ( image 3 c), There is no change in the relative position between the fiber cone and the nanowire, which demonstrates the good stability of the sensing structure. As time increases, the output light intensity gradually decreases, as image 3 d...
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