Method for constructing electrochemical luminescence aptamer sensor for omethoate detection

A technology of aptamer sensor and omethoate, which is applied in the field of electrochemical analysis and detection, can solve the problems of complex operation, unsuitability for large-scale sample processing and rapid detection of analysis, and expensive equipment, so as to achieve simple equipment and low detection cost. Inexpensive, low linear range effects

Active Publication Date: 2021-07-13
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Existing methods for detecting OMT include gas chromatography-mass spectrometry, enzyme inhibition, and high performance liquid chromatography-mass spectrometry. Although these methods can meet the requirements of sensitivity and specificity detection, they are not practical There are certain limitations
For example, chromatography can be used for qualitative and quantitative detection, and the detection results are relatively accurate and reliable, with high sensitivity and good reproducibility, but the equipment used is expensive, the operation is complicated, and professional technicians are required, so it is not suitable for large-scale samples. Processing and analysis and rapid detection in the field

Method used

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  • Method for constructing electrochemical luminescence aptamer sensor for omethoate detection
  • Method for constructing electrochemical luminescence aptamer sensor for omethoate detection
  • Method for constructing electrochemical luminescence aptamer sensor for omethoate detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1)MoTe 2 Preparation of quantum dots

[0038] Weigh 60mg of the purchased bulk MoTe 2 Place in a 40mL centrifuge tube and freeze in liquid nitrogen for 2 hours. Measure 10mL of tert-butanol and 10mL of water to mix as a co-solvent. The centrifuge tube was taken out and immediately added to the above co-solvent to form a mixed solution. Immediately transfer to a cell grinder and sonicate for 2 hours. Finally, the dispersion was centrifuged for 15 minutes at a speed of 13000rmp, and the supernatant was collected to obtain MoTe 2 Dispersions.

[0039] (2)MoTe 2 / Preparation of ZIF-8

[0040] Take 10mM methanol solution of 2-methylimidazole (HMeIM), add 1mL 1-10mg / L MoTe 2 The dispersion liquid was ultrasonically mixed for 0.5 hours to obtain solution A. Then take 10mM zinc nitrate (Zn(NO 3 ) 2 ) Methanol solution B, take 10mL of A and 10mL of B, mix thoroughly and let stand at room temperature for 2 hours. Centrifuge and wash with methanol 10000rmp 2-3 times....

Embodiment 2

[0052] (1)MoTe 2 Preparation of quantum dots

[0053] Weigh 40mg of the purchased bulk MoTe 2 Place in a 40mL centrifuge tube and freeze in liquid nitrogen for 2 hours. Measure 10mL of tert-butanol and 10mL of water to mix as a co-solvent. The centrifuge tube was taken out and immediately added to the above co-solvent to form a mixed solution. Immediately transfer to a cell grinder and sonicate for 2 hours. Finally, the dispersion was centrifuged for 15 minutes at a speed of 13000rmp, and the supernatant was collected to obtain MoTe 2 Dispersions.

[0054] (2)MoTe 2 / Preparation of ZIF-8

[0055] Take 10mM methanol solution of 2-methylimidazole (HMeIM), add 1mL 1-10mg / L MoTe 2 The dispersion liquid was ultrasonically mixed for 0.5 hours to obtain solution A. Then take 10mM zinc nitrate (Zn(NO 3 ) 2 ) methanol solution, take 10mL of A and 10mL of B, mix thoroughly and let stand at room temperature for 2 hours. Centrifuge and wash with methanol 10000rmp 2-3 times. ...

Embodiment 3

[0058] (1)MoTe 2 Preparation of quantum dots

[0059] Weigh 40mg of the purchased bulk MoTe 2 Place in a 40mL centrifuge tube and freeze in liquid nitrogen for 2 hours. Measure 10mL of tert-butanol and 10mL of water to mix as a co-solvent. The centrifuge tube was taken out and immediately added to the above co-solvent to form a mixed solution. Immediately transfer to a cell grinder and sonicate for 2 hours. Finally, the dispersion was centrifuged for 15 minutes at a speed of 13000rmp, and the supernatant was collected to obtain MoTe 2 Dispersions.

[0060] (2)MoTe 2 / Preparation of ZIF-8

[0061] Take 20mM methanol solution of 2-methylimidazole (HMeIM), add 1mL 1-10mg / L MoTe 2 The dispersion liquid was ultrasonically mixed for 0.5 hours to obtain solution A. Then take 10mM zinc nitrate (Zn(NO 3 ) 2 ) methanol solution, take 10mL of A and 10mL of B, mix thoroughly and let stand at room temperature for 2 hours. Centrifuge and wash with methanol 10000rmp 2-3 times. ...

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Abstract

The invention provides a method for constructing an electrochemical luminescence aptamer sensor for sensitively detecting omethoate. The method comprises the following steps: step 1, preparing molybdenum telluride (MoTe2) quantum dots; step 2, preparing a molybdenum telluride/2-methylimidazole zinc salt (MoTe2/ZIF-8) nano compound; and step 3, constructing the electrochemical luminescence aptamer sensor for sensitively detecting omethoate. The electrode material of the sensor adopts ZIF-8 with defects caused by quantum dots, so that the excitation potential of the material is effectively reduced, and the sensitivity of the sensor is greatly improved. Based on the excellent ECL performance of MoTe2/ZIF-8, an OMT aptamer with specific recognition is combined, and an ECL aptamer sensor is successfully prepared and used for OMT monitoring. Meanwhile, the electrochemical luminescence detection method of OMT provided by the invention has the characteristics of simplicity, convenience and flexibility in operation, simple instrument and equipment, wide detection range, low detection limit, low detection cost and the like.

Description

technical field [0001] The invention belongs to the field of electrochemical analysis and detection, and constructs an electrochemiluminescent aptamer sensor for detecting omethoate in vegetable samples. Background technique [0002] Organophosphorus pesticides (OPPs) refer to phosphorus-containing organic compound pesticides widely used in agricultural production, mainly as insecticides, and a few as herbicides, fungicides and plant growth regulators. It has played an active role in controlling agricultural diseases and insect pests and improving the quality and yield of agricultural products. Omethoate (OMT) is a kind of organophosphorus pesticide, which is widely used by farmers all over the world because of its advantages of broad spectrum, high efficiency, rapidity and low price. The long-term abuse of omethoate in large quantities has made the problem of pesticide residues increasingly serious. The residues of organophosphorus pesticides not only pollute the ecologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N21/76
CPCG01N27/26G01N27/308G01N21/76Y02A50/30
Inventor 王坤丁丽君郝楠
Owner JIANGSU UNIV
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