A kind of gold nanocage material that releases NO anti-MRSA biofilm in response to light and its preparation method and application

Abstract

The invention provides a photoresponsive release of NO anti-MRSA biofilm gold nanocage material and its preparation method and application, and relates to the field of biomedical engineering materials. The preparation method provided by the present invention utilizes the sulfhydryl groups on TCF to form gold-sulfur bonds with gold nanocage AuNC to realize the effective loading of NO donor TCF on gold nanocages, and the prepared AuNC@TCF will release slowly at physiological temperature NO, thereby promoting MRSA from the state of biofilm to the state of free bacteria. AuNC@TCF can be irradiated with near-infrared light to heat up rapidly, which can accelerate the release of NO from TCF, and further synergize with NO and photothermal therapy for antibacterial. The AuNC@TCF provided by the invention has obvious antibacterial and biofilm dissipating effects, and can be used as an antibacterial drug, wound healing promotion or anti-inflammatory drug, and shows an important application prospect in anti-biofilm.

Description

technical field

[0001] The invention relates to the field of biomedical engineering materials, in particular to a gold nanocage material capable of releasing NO in response to light and resisting MRSA biofilms, and a preparation method and application thereof. Background technique

[0002] Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically common and highly virulent bacterium, and it is one of the common drug-resistant bacteria. In addition to resistance to methicillin, MRSA has different degrees of drug resistance to aminoglycosides, macrolides, tetracyclines, fluoroquinolones, sulfonamides, rifampicin and even vancomycin. In addition to its own drug resistance, MRSA can also effectively protect itself through the formation of biofilms, which are highly resistant to antimicrobial agents and harsh environments. Once MRSA forms a biofilm in the organism, it is difficult to use conventional methods to sterilize it. Therefore, there is an urgent need for a saf...

Examples

Embodiment 1

[0034] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0035] (1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the prod...

Embodiment 2

[0049] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0050] (1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the prod...

Embodiment 3

[0054] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0055](1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the produ...

technical field

[0001] The invention relates to the field of biomedical engineering materials, in particular to a gold nanocage material capable of releasing NO in response to light and resisting MRSA biofilms, and a preparation method and application thereof. Background technique

[0002] Methicillin-resistant Staphylococcus aureus (MRSA) is a clinically common and highly virulent bacterium, and it is one of the common drug-resistant bacteria. In addition to resistance to methicillin, MRSA has different degrees of drug resistance to aminoglycosides, macrolides, tetracyclines, fluoroquinolones, sulfonamides, rifampicin and even vancomycin. In addition to its own drug resistance, MRSA can also effectively protect itself through the formation of biofilms, which are highly resistant to antimicrobial agents and harsh environments. Once MRSA forms a biofilm in the organism, it is difficult to use conventional methods to sterilize it. Therefore, there is an urgent need for a saf...

Examples

Embodiment 1

[0034] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0035] (1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the prod...

Embodiment 2

[0049] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0050] (1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the prod...

Embodiment 3

[0054] This embodiment discloses a method for preparing a gold nanocage material that releases NO in response to light and resists MRSA biofilms, comprising the following steps:

[0055](1) Preparation of AuNC: Heat 25mL of ethylene glycol to 150°C; keep at 150°C, 280rpm, quickly inject 0.3mL, 3mM NaHS solution into the above solution, and then add 2.5ml, 3mM hydrochloric acid solution at intervals of 2 minutes , 6.25mL, 20mg / mL of PVP solution and 2mL, 283mM of CF 3 COOAg solution, keep the original condition for 60min. Then the product was ice-bathed and then centrifuged with acetone (for the first time) and ultrapure water at 7500rpm to obtain AgNCs. Pour 50mL of ultrapure water into a round bottom flask, heat to 100°C while maintaining 250rpm, add 60μL, 5nM AgNCs, and then slowly add HAuCl dropwise to the mixed solution with a syringe pump 4 , and finally cooled in an ice bath, and then centrifuged with ultrapure water at 7500rpm for 3 times to obtain AuNC, and the produ...

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