Novel tissue culture seedling growing method for one-step seedling growing by utilizing polygala tenuifolia

A new method, Polygala technology, applied in the field of medicinal plant tissue culture, can solve the problems of restricting the development of Southwest Polygala planting and deep processing industry, low seedling rate of cutting propagation, cumbersome production technology, etc., and achieve high-efficiency explant sterile seedlings The effects of cultivation, reduction of disinfection contamination rate and disinfection mortality rate, and shortening of production cycle

Active Publication Date: 2021-08-06
INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the small number of seeds, low germination rate, and extremely low seedling rate of cutting propagation, the planting scale has always been small
In recent years, some units have carried out research on the propagation technology of tissue-cultured seedlings. Through literature review, it was found that the multiplication coefficient is low and the production technology is cumbersome. 3 to 5 yuan / plant, which greatly limits the development of polygala planting and deep processing industry in Southwest China

Method used

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  • Novel tissue culture seedling growing method for one-step seedling growing by utilizing polygala tenuifolia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Selection and cleaning of foreign implants: Select robust, diseaseless pest plants into the room, use a plug-in solution 2000 times liquid per day once, 7 days, after 7 days, take the young blade, placed in soap liquid After washing 20 minutes, rinse 3 times with tap water, then dropped 3 drop -80, shake 10 min, and finally use tap water to drag 60 min.

[0021] 2, the sterilization of the foreign implant: Transfer the clean young leaves, transfer to the ultra-net workbench, use 75% alcohol disinfection 30s, sterile water 2 times; then use a saturated bleach solution to disinfect 20 min, sterile water Wash 3 times; finally was sterilized with 0.05% increase in mercury, and sterilized 8 times. The 7-day disinfection pollution rate was 19.9%, and the 15-day blade mortality was 2.7%.

[0022] 3, an exemplary induction: the disinfecting blade, absorb the surface moisture with a sterile paper while cutting off the petio wound cutting surface, and then draws 5 ~ 8 wounds in th...

Embodiment 2

[0027] 1. Selection and cleaning of foreign implants: Select robust, diseaseless pest plants into the room, use a plug-in solution 2000 times liquid per day once, 7 days, after 7 days, take the young blade, placed in soap liquid After washing 25 min, rinse 3 times with tap water, then dropped 3 dropped Temperature-80, shake 12 min, and finally use tap water for 70 minutes.

[0028] 2, the disinfection of the foreign implant: will be cleaned and the young leaves are transferred to the ultra-net workbench, with 75% alcohol disinfection 35s, sterile water 2 times; then disinfected with a saturated bleach solution 30min, sterile water Wash 3 times; finally was sterilized with 0.05% increased by mercury and washed 8 times. The 7-day disinfected pollution rate was 17.8%, and the 15-day blade mortality was 4.1%.

[0029] 3, an exemplary induction: the disinfecting blade, absorb the surface moisture with a sterile paper while cutting off the petio wound cutting surface, and then draws 5 ~...

Embodiment 3

[0033] 1. Selection and cleaning of foreign implants: Select robust, diseaseless pest plants into the room, use a plug-in solution 2000 times liquid per day once, 7 days, after 7 days, take the young blade, placed in soap liquid After washing 25 min, rinse 3 times with tap water, then dropped 3 dropped Temperature-80, shake 12 min, and finally use tap water for 70 minutes.

[0034]2, the disinfection of the foreign implant: will be cleaned and the young leaves are transferred to the ultra-net workbench, with 75% alcohol disinfection 35s, sterile water 2 times; then disinfected with a saturated bleach solution 30min, sterile water Wash 3 times; finally was sterilized with 0.05% increased by mercury and washed 8 times. The 7-day disinfected pollution rate was 17.8%, and the 15-day blade mortality was 4.1%.

[0035] 3, an exemplary induction: the disinfecting blade, absorb the surface moisture with a sterile paper while cutting off the petio wound cutting surface, and then draws 5 ~ ...

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Abstract

The invention relates to the field of tissue culture of medicinal plants, and particularly discloses a novel tissue culture seedling growing method for one-step seedling growing by using polygala tenuifolia. The novel tissue culture seedling growing method comprises the following steps that explants are selected and cleaned; the explants are disinfected; seedling formation is induced; and domesticating is carried out, according to the explant induction culture, the disinfected explants are used as a basic culture medium through improved MS, NAA and TDZ are added, and tissue culture is carried out, specifically, NAA, brassinolide and TDZ are added to the induced cluster buds through the improved MS culture medium, and the improved MS culture medium is prepared by changing potassium nitrate to 1200 mg / L, ammonium nitrate to 1200 mg / L, monopotassium phosphate to 210 mg / L, magnesium sulfate to 130 mg / L and calcium chloride to 150 mg / L in macroelements in the MS culture medium; and meanwhile, the concentration of cane sugar is reduced to 20 g / L, and other elements are not changed. According to the novel tissue culture seedling growing method for one-step seedling growing by using polygala tenuifolia, the callus is generated during explant induction; and meanwhile, the callus is re-differentiated into the cluster buds, so that callus induction culture and cluster bud differentiation culture required in a culture process are reduced, finally, production links are reduced, and the production cost is reduced.

Description

Technical field [0001] The present invention relates to the field of pharmaceutical plant tissue culture, and in particular, a new method of cultivating seedlings in Southwestern Yuanzhi one step. Background technique [0002] Southwest Gula Crotalarioides Buch.-Ham. 西 西 远 志, 母, pig big intestines, for Yuanzhiko Yuanzhi is a perennial plants, born in hillside or shrub, born at 1100 ~ 2300m above, China mainly distributed In the north and west of Yunnan, southwestern Sichuan, southern Tibet. Southwest Yuanzhi takes the root into the medicine, mainly there is auxiliary cough, Ningxin's effect, mainly used to treat cough and cough, heart, palpitations, insomnia, more dreams. At the same time, the medicinal material is a kind of rare national medicine circulating in Yunnan people. It has the effect of promoting hyperthyroidism, strong muscle and promotion of appetite, and long-term use can make the eyebrows long, with colorful shin and sputum. Modern medicine pharmacological studies ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张金渝赵露琴曾祥飞杨维泽邓国宾起明菊
Owner INST OF MEDICINAL PLANTS YUNNAN ACAD OF AGRI SCI
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