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Application of pterostilbene in preparation of medicine for preventing and/or treating intestinal epithelial barrier injury

A technology for barrier damage and pterostilbene, which is applied in the application field of pterostilbene in the preparation of drugs for preventing and/or treating intestinal epithelial barrier damage, can solve problems such as undocumented PTE, and achieve the effect of reducing epithelial integrity damage in vitro

Pending Publication Date: 2021-08-06
迈迪唯希科技(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The prior art does not record the relevant technical content of the relationship between PTE and intestinal epithelial barrier protection

Method used

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  • Application of pterostilbene in preparation of medicine for preventing and/or treating intestinal epithelial barrier injury
  • Application of pterostilbene in preparation of medicine for preventing and/or treating intestinal epithelial barrier injury
  • Application of pterostilbene in preparation of medicine for preventing and/or treating intestinal epithelial barrier injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Protective effect of pterostilbene (PTE) on intestinal epithelial cells

[0047] 1. Culture of human colon cancer Caco2 cells

[0048] (1) Resuscitating cells: Take out the frozen Caco2 cell line from the liquid nitrogen tank, put it into warm water at 37°C and dissolve it quickly, then add the dissolved cell solution into a 15mL centrifuge tube containing 4mL of DMEM medium, and then Centrifuge at 1500rpm / min for 3min, discard the supernatant after the centrifugation is completed, add 1mL of DMEM medium and blow gently to suspend the cells in the medium, then add the cell suspension into a petri dish containing 5mL of DMEM medium, with the word "ten" Shake gently and place in 37°C containing 5% CO 2 Cultured in an incubator.

[0049] (2) Culture conditions: 37°C, 5% CO 2 , the culture medium is DMEM containing 10% fetal bovine serum (FBS), 2mg / L insulin and 1% penicillin and streptomycin mixed solution, and the culture medium needs to be changed every day.

[0050]...

Embodiment 2

[0064] cell monolayer permeability

[0065] The cell monolayer permeability is another important factor to measure whether the in vitro barrier model is damaged. It measures the passage rate of the fluorescently labeled macromolecular substance FITC-dextran (FITC-dextran) through the monolayer cells, which is used to evaluate the monolayer cell permeability.

[0066] The tight connection model established in Example 1 is used. After Caco2 cells were differentiated for 21 days, the Transwell chamber was slowly washed twice with PBS solution pre-warmed at 37°C, and then 100 μL of 0.1 mg / mL FITC was added to the AP layer of the Transwell chamber, and 600 μL of PBS buffer was added to the BL layer. in CO 2 After incubating in the incubator for 1 h, take 100 μL of liquid on the BL side and AP side of the Transwell respectively, and calculate the fluorescence absorbance with a multi-functional microplate reader (where the excitation wavelength is set at 480 nm, and the emission wa...

Embodiment 3

[0077] Study on the protective effect of PTE on the intestinal epithelial barrier in mice

[0078] Health Evaluation of a Mouse Model of Intestinal Injury

[0079] Experimental animal culture

[0080] 8-10 weeks old male C57BL / 6 mice, weighing 20-23 g, were purchased from Beijing Huafukang Biotechnology Co., Ltd. All the mice were placed in the Animal Experiment Center of the Institute of Radiation Medicine, Chinese Academy of Medical Sciences, raised at a room temperature of 23±3°C and a humidity of 50%±10%. Any disease was used in this experiment. All animal experiments were approved by the Ethics Committee of Tianjin University of Commerce.

[0081] Establishment of mouse intestinal injury model

[0082] Mice were randomly divided into 3 groups: normal control group, dextran sulfate sodium salt (DSS, molecular weight: 36-50kDa) treatment group, PTE group, 10 mice in each group. The blank control group had a normal diet, and the other two groups added 2.5% DSS to the drin...

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Abstract

The invention provides application of pterostilbene in preparation of a medicine for preventing and / or treating intestinal epithelial barrier injury, and belongs to the technical field of medicines. In the application, the pterostilbene protects the intestinal epithelial barrier by adjusting the expression level of tight junction protein. The protective effect of the stilbene natural product pterostilbene on intestinal epithelium is researched for the first time, and it is found that pterostilbene can regulate and control tight junction protein through a targeted NF-kappa B mediated MLCK-pMLC pathway, and the integrity of intestinal epithelial barrier is maintained, so that the intestinal permeability is reduced. As pterostilbene is rich in berry crops such as blueberries and grapes, reference is provided for culturing novel gastrointestinal functional foods based on high-valued processing and development of berry foods in the future.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of pterostilbene in the preparation of medicines for preventing and / or treating intestinal epithelial barrier damage. Background technique [0002] The intestinal epithelial barrier consists of the apical plasma membrane of intestinal epithelial cells and tight junctions between cells, which strictly regulates the absorption of water, ions and organic molecules in the epithelium. As an important characteristic function of the intestinal barrier, the relationship between intestinal permeability and the health and disease of the body has gradually attracted people's attention in recent years. Inflammatory mediators, flora imbalance, and high-fat diet are common factors that lead to the destruction of the intestinal mucosal barrier (including the epithelial barrier). Unfavorable harmful substances will enter the body through the intestinal tract and sta...

Claims

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Application Information

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IPC IPC(8): A61K31/09A61P1/00
CPCA61K31/09A61P1/00
Inventor 赵辉张美荣
Owner 迈迪唯希科技(天津)有限公司
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