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Endophytic fungus M-B927 and application thereof

A technology of M-B927, endophytic fungi, applied in the application, fungi, biocides, etc., to achieve the effect of enhancing disease resistance and reducing leaf damage

Active Publication Date: 2021-08-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report to point out the function of other endophytic fungal strains in wild rice in the control of rice seedling blast

Method used

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  • Endophytic fungus M-B927 and application thereof
  • Endophytic fungus M-B927 and application thereof
  • Endophytic fungus M-B927 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and Identification of Endophytic Fungi M-B927 Strain

[0026] 1. Isolation and purification of bacterial strains

[0027] M-B927 strain was isolated from the root system of O. yunnanensis. First, the wild rice roots are rinsed continuously with tap water to carefully remove soil particles and appendages. Select healthy root tissues, carry out surface disinfection treatment, soak in 75% ethanol for 1-2min, 1% sodium hypochlorite for 4-5min, and then wash with sterile deionized water for 3 times. Cut the root tissue pieces into 0.5cm sections, and then put them into 2% malt powder agar medium (MEA, malt extract agar, OXOID, add 50mg / L chloramphenicol to inhibit the growth of endophytic bacteria) and incubate at 25°C dark culture. On the 5th day of culture, endophytic fungal hyphae grew from the edge of the tissue incision, carefully picked out with an inoculation needle, transferred to fresh PDA medium for purification and culture, and recorded the ...

Embodiment 2

[0065] Example 2 M-B927 bacterial strain and rice root co-cultivation

[0066] Test plants: rice Oryza sativa L., conventional variety, CO39.

[0067] 1. M-B927 strain culture

[0068] The M-B927 strain preserved on the filter paper sheet was inoculated on the potato dextrose agar (PDA) solid medium for activation culture, 25°C, dark culture for 7 days, and set aside.

[0069] 2. Put the rice seeds in an Erlenmeyer flask after shelling them, disinfect the surface with 1% NaClO for 15 minutes, wash them with sterile water for 3 times, and set them aside. The sterilized seeds were spread evenly on 1 / 2MS (Murashige and Skoog) medium, sealed with Parafilm, and placed in a plant incubator at 25°C (16h light / 8h dark). After 3 days, the seeds turned white, and they were transferred to tissue culture bottles containing 1 / 2 MS medium, with 10 seeds per bottle. Insert 3 pieces of M-B927 bacteria cake (0.5cm in diameter) at the same time. The control group was sterile PDA agar block....

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Abstract

The invention discloses a Magnaporthaceae endophytic fungus M-B927 and application thereof, and belongs to the field of microorganism application. The preservation number of the endophytic fungus strain M-B927 is CCTCC (China Center For Type Culture Collection) M 2021503, and the endophytic fungus strain M-B927 is classified and named as Magnaporthaceae sp. The endophytic fungus strain M-B927 can improve the disease resistance of rice to leaf blast, the prevention and treatment effect reaches 73.06%, and the disease index is reduced by 52.22. The endophytic fungus M-B927 has a great value in popularization and application of the biological prevention and control effect on rice seedling leaf blast in the agricultural field.

Description

technical field [0001] The invention relates to the technical field of plant disease prevention and control, in particular to an endophytic fungus M-B927 of the genus Macropodium and its application in the prevention and treatment of rice blast. Background technique [0002] Rice blast is one of the important diseases of rice, which is caused by pathogens Phyricularia grisea (Cooke) Sacc. and Pyricularia oryae Cav. The disease is an epidemic disease transmitted by air currents, which poses a great threat to rice production. The degree of damage varies with varieties, cultivation techniques and climatic conditions. Generally, the yield is reduced by 10%-20%, and some fields have no harvest. Rice blast will occur throughout the rice growth period. According to the different time and location of the damage, it can be divided into seedling blast, leaf blast, leaf pillow blast, node blast, seedling leaf blast, branch blast and grain blast, among which seedling leaf blast Plague ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30C12N1/145C12R2001/645A01P3/00A01G13/00
Inventor 林福呈苏珍珠朱加楠
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES