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Method for producing glucose through enzymolysis of poplar

A glucose and enzymatic hydrolysis technology, which is applied in the field of poplar wood enzymatic hydrolysis to produce glucose, can solve the problems of long enzymatic hydrolysis time, large amount of cellulase, and shortened enzymatic hydrolysis time, so as to reduce the amount of enzyme, increase the yield of glucose, shorten The effect of enzymatic digestion time

Active Publication Date: 2021-08-10
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims at the problems of long enzymolysis time and large amount of cellulase in the enzymolysis process of producing poplar wood to produce bioenergy, and aims to provide a method for improving the enzymolysis efficiency of poplar wood through pretreatment with surfactant-assisted metal chloride , through a specific poplar pretreatment method, and then adding a specific surfactant to assist poplar enzymatic hydrolysis, on the premise of reducing the amount of enzyme and shortening the enzymatic hydrolysis time, it can also effectively improve the efficiency of enzymatic hydrolysis and increase the yield of glucose

Method used

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  • Method for producing glucose through enzymolysis of poplar
  • Method for producing glucose through enzymolysis of poplar
  • Method for producing glucose through enzymolysis of poplar

Examples

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Effect test

Embodiment 1

[0034] Embodiment 1 A kind of method that improves poplar enzymatic hydrolysis efficiency

[0035] S1. poplar wood pretreatment: 12g (absolute dry weight) poplar wood and 0.80g AlCl 3 Add 120mL of ultra-pure water, react in the reaction kettle at 180°C and 300rpm for 20min, add deionized water to wash until neutral, and use vacuum filtration to obtain poplar wood pretreatment residue;

[0036] S2. Enzymolysis: Take 2g poplar wood pretreatment residue (dry quantity), add 100mL acetic acid-sodium acetate buffer solution with pH=4.8, 40FPU cellulase (Saili II) and 0.3g surfactant PEG 8000, Enzyme hydrolyze at 50°C with a rotational speed of 150rpm.

Embodiment 2

[0037] Embodiment 2 A kind of method that improves poplar enzymatic hydrolysis efficiency

[0038] The difference from Example 1 is that the surfactant is replaced with 0.8 g of whey protein, and 100 mL of acetic acid-sodium acetate buffer solution with pH=4.5 is added.

Embodiment 3

[0039] Example 3 A method for improving the efficiency of enzymatic hydrolysis of poplar

[0040] The difference from Example 1 is that the surfactant is replaced by 0.2 g of calcium lignosulfonate, and 100 mL of acetic acid-sodium acetate buffer solution with pH=5 is added.

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Abstract

The invention provides a method for producing glucose by enzymolysis of poplar, the method comprises the following steps: firstly, pretreating the poplar by using metal chloride AlCl3 or CrCl3 under specific conditions, and then adding cellulase and a surfactant into pretreated residues for enzymolysis, by adding the surfactant to assist the pretreatment of the metal chloride, the enzymolysis efficiency and the glucose yield of the poplar are improved, the enzyme dosage is reduced, the enzymolysis time is shortened, the glucose yield after enzymolysis for 24 hours can reach up to 86.94% under the condition that the enzyme dosage is 17.5-20 FPU / g of poplar pretreatment residues, and the economic benefit is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of biomass pretreatment, and more specifically relates to a method for producing glucose by enzymatic hydrolysis of poplar wood. Background technique [0002] With the development of the times, energy and environmental issues have become increasingly prominent. Biomass energy, as a new renewable resource, has attracted more and more attention. Among them, lignocellulose is considered to be good because of its wide source, abundant reserves and inedibility. The raw material for preparing biofuel, the method of preparing liquid fuel from lignocellulosic biomass is to hydrolyze hemicellulose and cellulose in the raw material into monosaccharides such as xylose and glucose, and produce ethanol through fermentation. [0003] Poplar has a fast growth rate, short growth cycle, strong environmental adaptability, extensive planting area, and rich resources. It is an important raw material for the production of bioene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/14C12P19/02C13K1/02C12P2201/00
Inventor 谢君赵宸彪张红丹
Owner SOUTH CHINA AGRI UNIV
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