Composition for preventing browning of beverage and application of compositon

A composition and beverage technology, applied in the field of beverages, to achieve more absorption, prevent browning reaction, and easy absorption

Active Publication Date: 2021-08-13
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the basic and applied research on chlorella protein is still in its infancy, and the application and development of chlorella protein is of great significance to the comprehensive utilization of chlorella resources

Method used

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  • Composition for preventing browning of beverage and application of compositon
  • Composition for preventing browning of beverage and application of compositon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Method for preparing a small chlorolese renovation, including the following steps:

[0029] (1) The chloride powder and distilled water were mixed according to the ratio of 1: 5 (w / v), and the mixed liquid pH to 8.0 was adjusted with a NaOH solution to obtain a peline algae alkaline mixture;

[0030] (2) The alkaline mixture is ultrasonically crushed, and the power is 450 W, ultrasonic 4 S, and the interval 4 s. 6000 rpm centrifuge for 30 min, take the supernatant, vacuum freeze dry, and the chlorelicate bold A;

[0031] (3) EtOAc (EtOAc) EtOAc. After drying the precipitate, the chlorinated bold B;

[0032] (4) Chlorobutique B and distilled water is mixed according to 1: 5 (w / v), adding a neutral protease enzymatic solution; an enzymatic ratio of 2%; an enzymatic solution: temperature 50 ° C, an enzymatic solution Time is 2 ~ 16 h. The enzymatic solution is enzymatic, cooling. 8000 rpm was centrifuged for 10 min, and the supernatant was collected, and the vacuum freeze-...

Embodiment 2

[0034] Method for preparing a small chlorolese renovation, steps are as follows:

[0035] (1) The chloride powder and distilled water were mixed according to the ratio of 1: 8 (w / v), and the mixed liquid pH to 9.0 was adjusted with a NaOH solution to obtain a microcapalline base mixture;

[0036] (2) One time the alkaline mixture is equal to 2500 bar by high pressure homogeneous machine. 8000 rpm is centrifuged for 15 minutes, take the supernatant, spray dry, and the chlorelicate A;

[0037] (3) EtOAc (EtOAc) EtOAc. After drying the precipitate, the chlorinated bold B;

[0038] (4) The proportion of chlorinated bold B and distilled water is mixed according to 1: 8 (w / v), and an alkaline protease is added; the enzyme ratio is 2%; an enzymatic solution: temperature 55 ° C, enzymatic solution Time is 2 ~ 16 h. The enzymatic solution is an enzyme in a boiling water bath and cool. 8000 rpm was centrifuged for 10 min, and the supernatant was collected, and the spray was dried to obt...

Embodiment 3

[0040] Method for preparing a small chlorolese renovation, steps are as follows:

[0041] (1) The chloride powder and distilled water were mixed according to 1: 10 (w / v), and the mixed liquid pH to 8.0 was adjusted with a NaOH solution to obtain a microcapalline base mixture;

[0042] (2) The basic mixture is 500 bar, and the homogeneous pressure is 800 bar. 7000 rpm centrifuge for 20 min, take the supernatant, vacuum freeze dry, and the chlorinated bold A;

[0043] (3) EtOAc (EtOAc) Ethanol is added to the proportion of 1:10 (w / v), and mix well, and 7000 rpm is centrifuged for 20 min, and the supernatant is discarded. After drying the precipitate, the chlorinated bold B;

[0044] (4) The proportion of chloreli bold bonds and distilled water is mixed according to 1:10 (w / v), add papain protease enzymatic solution; enzymatic base ratio 2%; enzymatic solution: temperature 55 ° C, enzymatic hydrolysis time is 2 ~ 16 h. The enzymatic solution is an enzyme in a boiling water bath...

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Abstract

The invention discloses a composition for preventing browning of a beverage and application of the composition. The composition for preventing browning of the beverage provided by the invention comprises chlorella enzymatic hydrolysate and vitamin C. The composition can effectively prevent browning reaction of the fruit juice beverage, although research shows that vitamin C can inhibit browning, the inhibition effect is not obvious, and by adding the chlorella enzymatic hydrolysate, the browning prevention effect is remarkably improved. Besides, the chlorella enzymatic hydrolysate contains a large amount of active polypeptide components, and compared with common protein beverages in the market, the active polypeptide components are easier to absorb and play an active role.

Description

Technical field [0001] The invention belongs to the field of beverage, and more particularly to a composition for preventing browning of beverages. Background technique [0002] Beverages occupy a certain location in people's daily lives, the beverage consumer market is getting bigger and bigger, based on nutrition and taste, the current juice beverage is increasingly loved by consumers, while pure juice beverages are increasingly consumed due to the addition of large pigments and additives. There is a serious problem that is apparent, and studies have shown that the addition of D-heterormous acid sodium blood can prevent browning, but the effect of practical use is not obvious. [0003] Chlorella is a kind of single-cell green algae containing a large number of proteins, carbohydrates, vitamins, chlorophyll, minerals, and trace elements, and rich nutrients. Childlocks have many potential applications in various fields such as food, feed, medicine, and is listed by the United Nat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/58A23L33/125A23L33/18C12P21/06
CPCA23L2/58A23L33/125A23L33/18C12P21/06A23V2002/00A23V2200/048A23V2200/30A23V2250/55A23V2250/51A23V2250/708
Inventor 王彦超朱子昊薛长湖乔旭光侯虎
Owner OCEAN UNIV OF CHINA
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