Application of lignin as MALDI (matrix-assisted laser desorption ionization) matrix in small molecule substance detection

A technology of small molecular substances and small molecular compounds, which is applied in the application field of lignin as a MALDI matrix in the detection of small molecular substances, can solve the problems of cumbersome synthesis and modification of nanomaterials, difficulty in commercialization, and difficult separation and purification, and achieve Excellent optical absorption characteristics, overcoming matrix background interference, and low cost

Active Publication Date: 2021-08-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesis and modification of nanomaterials are cumbersome; reactive substrates usually target molecules with a certain type of specific functional groups, which have strong limitations; artificially synthesized macromolecular substrates are difficult to separate and purify, and the cost is high, making it difficult to achieve commercialization.

Method used

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  • Application of lignin as MALDI (matrix-assisted laser desorption ionization) matrix in small molecule substance detection
  • Application of lignin as MALDI (matrix-assisted laser desorption ionization) matrix in small molecule substance detection
  • Application of lignin as MALDI (matrix-assisted laser desorption ionization) matrix in small molecule substance detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Performance comparison between lignin and traditional organic substrates for detection of small molecular compounds

[0069] (1) Take a certain amount of glucose, prepare 1mg / mL aqueous mother liquor, and dilute according to the situation;

[0070] (2) Weigh a certain amount of DHB and CHCA, and prepare a 0.10mol / L methanol solution; weigh a certain amount of dealkalized lignin and alkaline lignin, and prepare a 30mg / mL aqueous mother liquor, and carry out according to the situation. dilution;

[0071] (3) Take 1 μL of the glucose solution in step 1 and 1 μL of the 15 mg / mL dealkalized lignin aqueous solution in step 2, respectively, and spot on the same spotting well of the target plate in sequence. Use a pipette gun to repeatedly pipette directly on the MALDI target plate, mix well, and dry naturally at room temperature;

[0072] (4) As a control, take 1 μL of glucose solution in step 1 and 1 μL of 0.1mol / L DHB methanol solution in step 2, 0.1mol / L CHCA...

Embodiment 2

[0075] Example 2: Dealkalized lignin is used as a matrix to optimize conditions for detection of small molecule compounds

[0076] (1) Prepare the mother liquor of 30mg / mL dealkalized lignin methanol solution and aqueous solution in Example 1, and dilute to obtain 5mg / mL, 10mg / mL, 15mg / mL, 20mg / mL and 25mg / mL dealkalized lignin prime solution;

[0077] (2) The thin-layer method is to point the matrix solution first, dry at room temperature, and then point the sample solution on this basis, and dry at room temperature again.

[0078] (3) The dry drop method is to sequentially spot the matrix and the sample solution on the same target plate spotting hole, mix by pipetting, and dry at room temperature.

[0079] (4) The target plate is sent to the MALDI mass spectrometer, and the positive ion reflection mode is selected for data collection.

[0080] In embodiment 2, figure 2 Systematic optimization of conditions for the detection of small molecule compounds in a lignin matri...

Embodiment 3

[0081] Example 3: Qualitative detection of small molecule compounds in dealkalized lignin

[0082] (1) with ddH 2 O is the solvent, prepare 1 mg / mL of arabinose, valine, methionine, lysine, arginine, malic acid, citric acid, phenyllactic acid, acetylsalicylic acid, uridine, 2'- Deoxyadenosine, 2'-deoxyguanosine, deoxycytosine and β-thymidine in aqueous solution; with ddH 2 O as a solvent, prepare 4mM maltose, maltotriose and maltohexaose aqueous solutions; use 5mg / mL sodium chloride aqueous solution as solvent, prepare 1mg / mL vitamin B2; use ethanol as solvent, prepare 1mg / mL palmitate acid, stearic acid, linoleic acid, diethyl malonate, vitamin E, triacetin, dioctyl phosphate, tributyrin, glyceryl stearate and cholesterol in ethanol;

[0083] (2) 15mg / mL dealkalized lignin aqueous solution in the preparation embodiment 2;

[0084] (3) adopting the thin-layer method in embodiment 2 to carry out sampling and drying;

[0085] (4) The target plate is sent to the MALDI mass ...

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Abstract

The invention discloses application of lignin as an MALDI (matrix-assisted laser desorption ionization) matrix in small molecule substance detection. Small molecule compounds are saccharides, glycosides, esters, vitamins, amino acids, hydroxy acids or fatty acid small molecule compounds, and m / z of the small molecule compounds is smaller than 1000. Lignin has an excellent optical absorption characteristic and is difficult to ionize in mass spectrum, so that the defect that matrix background interference is easily generated in a low molecular weight interval of a traditional small organic molecule matrix can be overcome. In the MALDI mass spectrum, the rapid qualitative analysis of various small molecule compounds can be achieved based on the lignin matrix. In addition, accurate quantification of small molecular substances can be achieved by adopting an internal standard method. Through nuclear magnetic characterization and signal attribution, a main structural unit playing a matrix role in lignin can be determined.

Description

technical field [0001] The invention relates to the field of small molecule analysis of MALDI mass spectrometry, in particular to the application of lignin as a MALDI matrix in the detection of small molecule substances. Background technique [0002] Matrix-assisted laser desorption ionization (MALDI) and electrospray ionization (ESI) are soft ionization mass spectrometry technologies invented in the 1980s. The invention and application of soft ionization mass spectrometry technology make mass spectrometry analysis objects From volatile, thermally stable small molecular compounds to polar macromolecules that are difficult to volatilize and have poor thermal stability, the development of mass spectrometry has entered the stage of biological mass spectrometry. In 1988, Tanaka used a mixture of glycerol and cobalt powder as a MALDI matrix to detect a complete protein signal with a molecular weight of more than 3.4kDa for the first time, and won the 2002 Nobel Prize in Chemistry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 赵晓勇潘远江李鲜王慧文
Owner ZHEJIANG UNIV
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