Recombinant novel coronavirus RBD tripolymer protein vaccine capable of generating broad-spectrum cross-neutralization activity as well as preparation method and application of recombinant novel coronavirus RBD tripolymer protein vaccine

A coronavirus and trimer technology, applied in the field of biomedicine, can solve the problems of weakening the affinity of neutralizing antibodies, enhancing the virulence and infectivity of the virus, and reducing the protective effect of vaccines.
CN113292640AActive Publication Date: 2021-08-24NAT VACCINE & SERUM INST
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Patent Information

Authority / Receiving Office
CN Ā· China
Current Assignee / Owner
NAT VACCINE & SERUM INST
Publication Date
2021-08-24

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Abstract

The invention discloses a recombinant RBD trimer protein capable of simultaneously generating cross neutralization activity aiming at multiple novel coronavirus (SARS-CoV-2) epidemic strains, the trimer protein is composed of subunits of three novel coronavirus S protein RBD regions, and the amino acid sequences of the three novel coronavirus RBD regions are the same or at least one is different; and when the amino acid sequences of the three novel coronavirus RBD regions are the same, the amino acid sequences are the amino acid sequences shown as SEQ ID No.2 or SEQ ID No.3, or sequences with more than 95% of homology with the amino acid sequences shown as SEQ ID No.2 or SEQ ID No.3. The RBD trimer protein is taken as an antigen and supplemented with an adjuvant to immunize an organism, so that a high-titer neutralizing antibody aiming at various novel coronavirus epidemic strains can be generated at the same time, and the antibody has certain broad spectrum and can be used for treating and / or preventing novel coronavirus infection and / or novel coronavirus diseases.
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Description

technical field

[0001] The invention relates to the field of biomedicine, in particular to a recombinant novel coronavirus RBD trimer protein vaccine producing broad-spectrum cross-neutralizing activity, its preparation method and application. Background technique

[0002] Novel coronavirus (SARS-CoV-2), belonging to Nidovirales, Coronaviridae, Orthocoronavirus subfamily, Betacoronavirus genus, Sarbecovirus subgenus, SARS-like virus species, single-stranded positive-sense RNA virus , with an envelope, the full length of the genome is about 29.9kb, most of which encode non-structural proteins, involved in virus replication and translation, and a small part of the sequence encodes structural proteins, such as: S protein (spike protein,), M protein (membrane protein), E protein (envelope protein) and N protein (nucleo protein). In addition, there are several accessory proteins: 3a, 3b, p6, 7a, 7b, 8b, 9b and orf14, which are all involved in virus assembly. The S, M and E prot...

Claims

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