Organ-like forming device and working method thereof

A molding device and working method technology, applied in the field of biomedicine, can solve the problems of low cell concentration, high cell concentration, concentration difference, etc., and achieve the effects of high degree of automation, improved success rate, and shortened induction time.

Pending Publication Date: 2021-08-24
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the existing method of injecting samples with a syringe to obtain the sample droplets of cell suspension for organoid culture, the cells will settle at the bottom of the syringe due to their own gravity, resulting in a high concentra

Method used

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  • Organ-like forming device and working method thereof
  • Organ-like forming device and working method thereof
  • Organ-like forming device and working method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] like image 3 and Figure 4 As shown, an organoid forming device includes a pump 1, a helical tube 2 connected to the pump 1 at one end and used for storing and transporting cell suspension, and connected to the other end of the helical tube 2 through air jet or high pressure. The electrostatic action extrudes the cell suspension transported by the spiral tube 2 to form the nozzle unit 3 of the cell suspension microspheres. In the present invention, the spiral tube 2 is a medical silicone rubber tube with an inner diameter of 0.7-3.6mm, and the liquid in it maintains a certain state of stability due to the adhesion with the tube wall and capillary adsorption, and does not It will flow spontaneously, so that the cell suspension can be stored stably in the spiral tube 2, so as to ensure the uniformity of the cell concentration during the formation of the cell suspension microspheres, and finally make the constructed organoids uniform in size, suitable for high-throughput...

Embodiment 2

[0051] This embodiment is similar to Embodiment 1, the difference is that, as Image 6 and Figure 7 As shown, the spray head unit 3 includes a spray head hose 31 arranged at the other end of the spiral tube 2, a needle 32 connected to the end of the spray head hose 31 away from the spiral tube 2, and set on the A third pinch valve 37 on the nozzle hose 31 close to the needle 32 is connected to a high-voltage direct current source 38 . The nozzle hose 31 is actually integrated with the spiral tube 2. It is also a medical silicone rubber tube. The needle 32 is a steel needle 32. The high-voltage DC source 38 can connect the needle 32 to a high-voltage DC of 1kv-15kv. A high voltage electrostatic field is formed below.

[0052] like Figure 7 As shown, the needle head 32 is provided with a wiring clamp 39, the high-voltage DC source 38 is connected to the wiring clamp 39 through wires, and the pump 1 is a constant-current pump 1 .

[0053] like Image 6 As shown, the presen...

Embodiment 3

[0055] A working method based on the organoid forming device described in embodiment 1, wherein, comprising the following steps:

[0056] S1. The controller 4 controls the first pinch valve 33 and the second pinch valve 34 to open, and the controller 4 controls the pump 1 to rotate in reverse to suck the cell suspension containing cells or microtissues into the spiral tube 2 through the nozzle hose 31 in use;

[0057] S2. The controller 4 controls the first pinch valve 33 to keep open, the second pinch valve 34 to close, and the pump 1 rotates forward for one beat to squeeze the cell suspension from the spiral tube 2 to fill the space of the nozzle hose 31;

[0058] S3. The controller 4 controls the first pinch valve 33 to close, the second pinch valve 34 to open, the controller 4 controls the solenoid valve 36 to open, and the air pump 35 releases the filtered high-pressure gas through the needle 32, so that the nozzle hose A certain volume of cell suspension in 31 is pushed...

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Abstract

The invention provides an organ-like forming device. The organ-like forming device comprises a pump, a spiral pipe and a spray head unit, one end of the spiral pipe is connected with the pump, the spiral pipe is used for storing and transporting cell suspension, and the spray head unit is connected to the other end of the spiral pipe and extrudes the cell suspension conveyed by the spiral pipe to form cell suspension microspheres through the airflow spraying effect or the high-voltage electrostatic effect. The invention further provides a working method of the organoid forming device. According to the method, the initial cell content of the obtained cell suspension sample liquid drops for organoid culture is uniform and consistent, so that the finally constructed organoid body is uniform in size.

Description

technical field [0001] The invention relates to the field of biomedical technology, and more specifically, to an organoid forming device and a working method thereof. Background technique [0002] Organoids are a model based on a 3D in vitro cell culture system that is highly similar to the source tissue or organ in vivo. These 3D in vitro culture systems can replicate the complex spatial morphology of differentiated tissues, and can show the interaction and spatial position morphology between cells and between cells and their surrounding matrix. Its characteristics must contain more than one cell type identical to the source organ; it should exhibit some functions unique to the source organ; the organization of cells should be similar to that of the source organ. Organoid technology has applications in a broad range of fields, including developmental biology, disease pathology, cell biology, mechanisms of regeneration, precision medicine, and drug toxicity and efficacy tes...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/36C12M1/26C12M1/04
CPCC12M29/06C12M33/04C12M41/00C12M41/48
Inventor 刘利彪邓坤学袁玉宇
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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