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Essence containing stem cell exosome and preparation method of essence

A technology of stem cells and exosomes, applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve the problems of slow effect, improve the degree of fineness, inhibit oxidation and the growth of microorganisms, and improve the ability to lock water Effect

Pending Publication Date: 2021-08-27
上海南滨江细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, simply using stem cell extracts to achieve cosmetic effects through injection is slow, and the injection method requires professionals to use medical devices in designated places, which limits its audience. Exosome essence products are also rare, so the development of an essence containing stem cell exosomes with moisturizing, whitening and anti-oxidant properties can solve this problem

Method used

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  • Essence containing stem cell exosome and preparation method of essence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Modified collagen is prepared by the following steps:

[0035] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 30 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;

[0036] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20 g / L solution, raising the temperature to 50° C. for 30 minutes to obtain a chitosan solution;

[0037] Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 20 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;

[0038] Step S4, m...

Embodiment 2

[0040] Modified collagen is prepared by the following steps:

[0041] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 30 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;

[0042] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20 g / L solution, raising the temperature to 50° C. for 30 minutes to obtain a chitosan solution;

[0043] Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 20 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;

[0044] Step S4, m...

Embodiment 3

[0046] Modified collagen is prepared by the following steps:

[0047] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 40 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;

[0048] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20g / L solution, raising the temperature to 50°C for 40min to obtain a chitosan solution;

[0049]Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 24 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;

[0050] Step S4, mix the co...

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Abstract

The invention discloses essence containing stem cell exosomes, and belongs to the technical field of cosmetic preparation. The essence comprises the following raw materials in parts by weight: 10-13 parts of skin stem cells, 15-20 parts of sodium hyaluronate, 6-8 parts of denatured ethanol, 2-6 parts of hydroxyethyl piperazine ethanesulfonic acid, 5-8 parts of modified collagen, 4-6 parts of organic silicon wax, 3-5 parts of octyldodecanol, 2-4 parts of ascorbyl glucoside, 1-3 parts of ammonium polyacryloyldimethyl taurate and 10-12 parts of deionized water. The invention also discloses a preparation method of the essence. According to the skin stem cell exosome essence, the skin stem cell exosome is used in the essence, the water locking capacity of the essence on the skin can be greatly improved, the fine and smooth degree of the skin can be improved, and meanwhile, the modified collagen is rich in collagen, chitosan and camellia seed extracts, so that the skin stem cell exosome essence not only has high nutritional value, but also has the characteristics of moisture resistance, oxygen resistance, bacteria resistance, oxidation resistance and moisture retention.

Description

technical field [0001] The invention belongs to the technical field of cosmetic preparation, and relates to an essence containing stem cell exosomes and a preparation method thereof. Background technique [0002] Skin is the largest organ of the human body. It covers the body surface and is composed of epidermis, dermis, subcutaneous tissue and appendages. It plays an important role in resisting microbial invasion, ultraviolet radiation, preventing water loss, regulating body temperature and maintaining human appearance. important role. In recent years, the use of living cells for beauty has become a hot spot in the market, especially the use of stem cells. Through direct injection on wrinkles, scars and scars, living cells can differentiate and grow in the skin, filling and repairing the skin. [0003] However, simply using stem cell extracts to achieve cosmetic effects through injection is slow, and the injection method requires professionals to use medical devices in des...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/98A61K8/9789A61K8/73A61K8/65A61Q19/00A61Q19/02A61Q19/08
CPCA61K8/99A61K8/985A61K8/9789A61K8/736A61K8/65A61Q19/00A61Q19/02A61Q19/08
Inventor 占震锋李庆静
Owner 上海南滨江细胞生物科技有限公司
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