Essence containing stem cell exosome and preparation method of essence
A technology of stem cells and exosomes, applied in skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve the problems of slow effect, improve the degree of fineness, inhibit oxidation and the growth of microorganisms, and improve the ability to lock water Effect
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Embodiment 1
[0034] Modified collagen is prepared by the following steps:
[0035] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 30 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;
[0036] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20 g / L solution, raising the temperature to 50° C. for 30 minutes to obtain a chitosan solution;
[0037] Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 20 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;
[0038] Step S4, m...
Embodiment 2
[0040] Modified collagen is prepared by the following steps:
[0041] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 30 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;
[0042] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20 g / L solution, raising the temperature to 50° C. for 30 minutes to obtain a chitosan solution;
[0043] Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 20 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;
[0044] Step S4, m...
Embodiment 3
[0046] Modified collagen is prepared by the following steps:
[0047] Step S1, weighing collagen in a beaker, adding deionized water to prepare a 20g / L solution, raising the temperature to 50°C for 40 minutes, and removing the precipitate by suction filtration to obtain a collagen solution;
[0048] Step S2, adding acetic acid solution with a volume fraction of 2% to the chitosan to prepare a 20g / L solution, raising the temperature to 50°C for 40min to obtain a chitosan solution;
[0049]Step S3, crushing camellia oleifera seeds into 40-mesh camellia oleifera seed powder, adding petroleum ether to the camellia oleifera seed powder, stirring and reacting at room temperature for 24 hours, standing still for 6 hours, suction filtration, taking the supernatant liquid and rotary steaming to concentrate to three minutes of the original volume One, to obtain camellia seed extract, wherein the mass ratio of camellia seed powder to petroleum ether is 1:10;
[0050] Step S4, mix the co...
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