Bionic nano-drug co-loaded with JTC801 and DNA methylated transferase inhibitor and preparation method and application thereof

A JTC801, bionic nanotechnology, applied in the field of medicine, can solve the problems of cancer and cancer pain that cannot be treated simultaneously, and achieve the effect of achieving simultaneous treatment, promoting enrichment, and increasing accumulation

Pending Publication Date: 2021-08-27
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above situation, in order to overcome the defects of the prior art, the object of the present invention is to provide a biomimetic nano-medicine co-carryi

Method used

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  • Bionic nano-drug co-loaded with JTC801 and DNA methylated transferase inhibitor and preparation method and application thereof
  • Bionic nano-drug co-loaded with JTC801 and DNA methylated transferase inhibitor and preparation method and application thereof
  • Bionic nano-drug co-loaded with JTC801 and DNA methylated transferase inhibitor and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0033] A preparation method of M1 macrophage membrane biomimetic nanomedicine co-loaded with JTC801 and azacitidine gold nanocage, comprising the following steps:

[0034](1) Synthesis of silver nanoparticles (AgNPs): Add 10 mL of 10 mg / mL citrate solution and 37.5 mL of deionized water into a round bottom flask, heat the mixture to 70 °C, and after 15 min, add 10 mg / mL AgNO 3 Solution 850 μL, while quickly adding 1 mg / mL NaBH 4 solution 200 μL, the reaction solution was vigorously stirred at 70 °C for 1 h, and then cooled to room temperature naturally to obtain AgNPs with small particle size as the seed solution, and then 1 mL of 10 mg / mL citrate solution was mixed with 37.5 mL of water , after boiling for 15 min, add 5 mL of seed solution and 850 μL of 10 mg / mL AgNO at the same time 3 Solution, under reflux conditions, continue to stir for 1 h, then add 1 mL of 10 mg / mL citrate solution and 850 μL of 10 mg / mL AgNO 3 solution, stirred and refluxed for 1 h, repeated once, ...

Embodiment 2

[0041] A method for preparing M1 macrophage membrane biomimetic nanomedicine co-loaded with JTC801 and DNA methyltransferase inhibitors, wherein the inner core of the nanoparticle is hyaluronic acid-deoxycholic acid, comprising the following steps:

[0042] (1) Synthesis of HA-DOAC (hyaluronic acid-deoxycholic acid): using ethylenediamine as a linker to connect hydrophilic hyaluronic acid (1-20 kDa) and hydrophobic deoxycholic acid through amide reaction, get HA-DOAC;

[0043] (2) Preparation of drug-loaded HAD NPs: HA-DOAC, JTC801, and DNA methyltransferase inhibitor at a mass ratio of 4:2:1 were dissolved in formamide so that the final concentration of HA-DOAC was 20~ 40 mg / mL was added dropwise to the aqueous solution under stirring conditions, and finally the mass fraction of HA-DOAC was 4%~6%. The solution was dialyzed to remove the organic solvent formamide and free drugs to obtain the inner core of DJHAD nanoparticles;

[0044] (3) Extraction and differentiation of ma...

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Abstract

The invention provides a bionic nano-drug co-loaded with JTC801 and a DNA methylated transferase inhibitor. The bionic nano-drug comprises an M1 type macrophage membrane, a nanoparticle core, the JTC801 and the DNA methylated transferase inhibitor, the JTC801 and the DNA methylated transferase inhibitor are simultaneously loaded in the nanoparticle core, and then the surface of the nanoparticle core is coated with the M1 type macrophage membrane; the enrichment of the M1 type macrophage bionic system at the tumor site is further promoted, the targeted amplification effect is achieved, on one hand, the nanoparticles are disguised as endogenous substances through the bionic effect of the M1 type macrophage cell membrane so that the nanoparticles are protected from being swallowed by the reticuloendothelial system; on the other hand, the macrophages can be rapidly enriched to tumor tissues due to recruitment effect, accumulation of drugs at tumor parts is increased, synchronous treatment of cancers and cancer-related pains is realized, and the drug is an innovation of synchronous treatment drugs for cancers and cancer-related pains, and has huge social and economic benefits.

Description

technical field [0001] The invention relates to the field of medicine, and relates to the construction of an M1 macrophage membrane biomimetic system and the combined application of antitumor drugs, in particular to a biomimetic nano drug co-carrying JTC801 and a DNA methyltransferase inhibitor, its preparation method and application. Background technique [0002] With the advancement and development of medical science and technology, despite great breakthroughs in the treatment of tumors in recent years, malignant tumors are still one of the diseases with the highest morbidity and mortality in the world. Moreover, the development of cancer, the toxic side effects of various therapies, and cancer-related pain seriously affect the quality of life of cancer patients. Almost all cancer patients are accompanied by cancer pain, but cancer pain has always been regarded as a result of cancer development, and clinical cancer pain treatment and cancer treatment have always been indep...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K31/4706A61K47/02A61K47/36A61K47/46A61P25/00A61P29/00A61P35/00B82Y5/00B82Y40/00A61K31/706A61K31/7068
CPCA61K31/4706A61K47/02A61K47/36A61K31/706A61K31/7068A61K9/5115A61K9/5068A61P35/00A61P25/00A61P29/00B82Y5/00B82Y40/00A61K2300/00
Inventor 王蕾郑翠霞刘欣欣孔月月冯倩华宋庆龄赵洪娟
Owner ZHENGZHOU UNIV
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