Bionic nano-drug co-loaded with JTC801 and DNA methylated transferase inhibitor and preparation method and application thereof
A JTC801, bionic nanotechnology, applied in the field of medicine, can solve the problems of cancer and cancer pain that cannot be treated simultaneously, and achieve the effect of achieving simultaneous treatment, promoting enrichment, and increasing accumulation
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Embodiment 1
[0033] A preparation method of M1 macrophage membrane biomimetic nanomedicine co-loaded with JTC801 and azacitidine gold nanocage, comprising the following steps:
[0034](1) Synthesis of silver nanoparticles (AgNPs): Add 10 mL of 10 mg / mL citrate solution and 37.5 mL of deionized water into a round bottom flask, heat the mixture to 70 °C, and after 15 min, add 10 mg / mL AgNO 3 Solution 850 μL, while quickly adding 1 mg / mL NaBH 4 solution 200 μL, the reaction solution was vigorously stirred at 70 °C for 1 h, and then cooled to room temperature naturally to obtain AgNPs with small particle size as the seed solution, and then 1 mL of 10 mg / mL citrate solution was mixed with 37.5 mL of water , after boiling for 15 min, add 5 mL of seed solution and 850 μL of 10 mg / mL AgNO at the same time 3 Solution, under reflux conditions, continue to stir for 1 h, then add 1 mL of 10 mg / mL citrate solution and 850 μL of 10 mg / mL AgNO 3 solution, stirred and refluxed for 1 h, repeated once, ...
Embodiment 2
[0041] A method for preparing M1 macrophage membrane biomimetic nanomedicine co-loaded with JTC801 and DNA methyltransferase inhibitors, wherein the inner core of the nanoparticle is hyaluronic acid-deoxycholic acid, comprising the following steps:
[0042] (1) Synthesis of HA-DOAC (hyaluronic acid-deoxycholic acid): using ethylenediamine as a linker to connect hydrophilic hyaluronic acid (1-20 kDa) and hydrophobic deoxycholic acid through amide reaction, get HA-DOAC;
[0043] (2) Preparation of drug-loaded HAD NPs: HA-DOAC, JTC801, and DNA methyltransferase inhibitor at a mass ratio of 4:2:1 were dissolved in formamide so that the final concentration of HA-DOAC was 20~ 40 mg / mL was added dropwise to the aqueous solution under stirring conditions, and finally the mass fraction of HA-DOAC was 4%~6%. The solution was dialyzed to remove the organic solvent formamide and free drugs to obtain the inner core of DJHAD nanoparticles;
[0044] (3) Extraction and differentiation of ma...
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