Transferring and dispersing method for thin-wall conidia of ustilaginoidea virens

Abstract

The invention discloses atransferring and dispersing method for thin-wall conidia of ustilaginoidea virens. Spores are picked up from a spore-forming colony and are directly transferred and dispersed to an observation plate. The method comprises the following steps: 1) preparation of materials and appliances; 2) preparation of a spore source; 3) preparation of a spore observation plate; 4) picking of spores and diluting of dense spores; 5) transferring and releasing of the spores; and 6) culturing and observing of the spores. The method has the following advantages: 1) a key working material (thin-wall conidia) does not need to be prepared into a spore suspension, so that the pollution risk is reduced; and 2) the germination and growth of thin-wall conidia can be dispersed and observed on the long-strip-shaped culture plate, so that the efficiency and effect of observing spore germination activity are improved, and multi-factor action design and batch test comparison in spore germination biological research are facilitated.

Description

technical field [0001] The invention relates to plant pathology technology, in particular to a method for transferring and dispersing conidia of the rice false smut fungus parenchyma. Background technique [0002] Rice false smut is an important disease of rice production in my country, which can cause heavy losses to rice production. The disease is caused by the infection of Ustilaginoidea virens. Thin-walled conidia are the asexual propagules of rice false smut, which play an important role in the disease cycle and epidemic of rice false smut. It is very important to fully understand the germination biology of parenchyma conidia to effectively control the occurrence and epidemic of rice false smut. In the germination biology of rice mistletoe propagules and related research work, it is often necessary to transplant / transfer the parenchyma conidia to the culture plate for observation and culture. This observation and culture not only requires the spores on the culture plat...

AI Technical Summary

Problems solved by technology

This method seems to be simple, but there are still considerable difficulties and tediousness in practical operation, which is mainly manifested in that in the technical operation of obtaining key working materials (parenchyma conidia), it is often necessary to take filtration operations to remove the spores from the spores. The hyphae of the spore liquid also need centrifugal sedimentation to remove the medium and culture metabolites in the spore liquid. These operations have a greater risk of contamination. In addition, the spore concentration of the spore liquid needs to be adjusted to an appropriate range. Therefore, it is necessary to Repeated preparation and sampling to the counting plate or glass slide, and transfer to the biological microscope for observation and counting, which is cumbersome

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