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A kind of Lactobacillus paracasei b111h and its application in metabolic syndrome

A technology for metabolic syndrome and paracheese, applied in metabolic diseases, medical preparations containing active ingredients, bacteria, etc., can solve the problems of side effects, high price, etc., to reduce body weight and blood lipid levels, inhibit lipid accumulation, excellent The effect of hypolipidemic ability

Active Publication Date: 2021-11-05
BEIJING QUANTIHEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, statins are considered to be the most effective lipid-lowering drugs at present, and have good effects in clinical treatment, but they need to be taken for a long time, and they are expensive and accompanied by different degrees of side effects

Method used

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  • A kind of Lactobacillus paracasei b111h and its application in metabolic syndrome
  • A kind of Lactobacillus paracasei b111h and its application in metabolic syndrome
  • A kind of Lactobacillus paracasei b111h and its application in metabolic syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Strain isolated in Example 1 having a hypolipidemic effect. Embodiment

[0043] Collection of stool Hainan Chengmai number of centenarians in the world's longevity as a separate sample of the object before the acquisition have not been taking antibiotics, probiotics taking no history, no history of gastrointestinal collected.

[0044] Extraction stool sample dilutions collected from 5 ml, 10-fold serial dilutions to 10 -6 , 200μl of each dilution is applied to take selective MRS medium (tryptone 10 g, beef extract 10 g, yeast extract 5 g, diammonium hydrogen citrate 2 g, glucose 20 g, Tween 80 1 mL, sodium acetate 5 g, dipotassium hydrogen phosphate 2 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g, agar 18 g, distilled water 1 000 mL), 37 ℃ anaerobic culture 24-48h, different isolated single colonies on the plate. With an inoculating loop after sterilization picked different single colonies were streaked on new purified MRS solid medium plates, 37 ° C anaerobic cultu...

Embodiment 2

[0046] Preparation Example 2 To live bacteria, metabolites and inactivated bacteria embodiment

[0047] 2.1 culture bacteria

[0048] -80 ℃ frozen after taking on MRS broth solids coated plates, cultured inverted 24-48h at 37 ℃, a single colony was inoculated in MRS liquid medium and cultured for 18-24h 37 ℃, generation of bacteria obtained ; generation of bacteria inoculum according to take a 10% concentration to fresh MRS broth, the second generation broth obtained after cultivation 18-24h 37 ℃; then take the second generation 10% strength by bacteria inoculated in fresh MRS broth in, 37 ℃ obtain work 18-24h culture broths.

[0049] 2.2 live bacteria obtained

[0050] In one particular embodiment of the present invention, the work of bacteria obtained in step 1 was placed 13000 rpm, 15 min after centrifugation at 4 ℃, the supernatant was discarded, the precipitate was collected, resuspended with saline to obtain the bio the bacteria live bacteria.

[0051] Incidentally, live bac...

Embodiment 3

[0056] Example 3. Strains Screening and Identification embodiment having a lipid-lowering effect

[0057] 3.1 Oil Red O staining experiments

[0058] The present invention uses a source HepG2 cells were purchased from the National Biomedical Library experimental cell culture method of culturing a manner conventionally used in the art based, in one embodiment of the present invention, the culture way: The human hepatoma HepG2 cells at 37 [deg.] C and 5% CO 2 Cultured in high glucose DMEM containing 10% fetal bovine serum (FBS) in the culture medium under a condition of 1: 100 anti-bis added (100 μg / ml penicillin and 100 μg / ml streptomycin) per 1 -2d replaced once with fresh medium, the cells covered by the 1: 3-4 subcultured.

[0059] The effect of lipid accumulation model strain in the present invention provides a construction HepG2 cells was observed in strains of the present invention provides an impact on lipid metabolism, as follows:

[0060] Good growth state of taking th...

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Abstract

The invention provides Lactobacillus paracasei B111H and its application in metabolic syndrome, and relates to the technical field of microorganisms and its application. The strain of Lactobacillus paracasei is preserved in the General Microbiology Center of the China Committee for the Collection of Microorganisms, with the preservation number: CGMCC No: 22184; the preservation time is: April 14, 2021. Lactobacillus paracasei of the present invention is isolated and screened from human feces, has the effect of significantly inhibiting lipid accumulation in liver cells, reducing body weight and blood lipid levels, and improving hepatic steatosis. The occurrence of liver has important application significance. The discovery of the strain provides a new microbial resource for lipid-lowering foods or medicines.

Description

Technical field [0001] The present invention relates to the field of microorganisms and their applications, in particular, to a subharidacillus ( Lactobacillus Paracsei Application of B111H and Its Application in Metabolic Syndrome. Background technique [0002] With the improvement of people's living standards, the changes in food structure and the speed of life, the pressure increase, "three high" problems are gradually serious, only one blood lipid is anomalies, and the prevalence is as high as 40.4%. The blood lipid is not only the exclusive disease of the elderly, but the patient group gradually became a "first killer" in the middle-aged population of 30 to 50 years old, greatly aggravated the serious situation of prevention and control of blood lipid abnormal diseases in my country. Blood lipid is an important risk factor that leads to cardiovascular and cerebrovascular diseases, like high blood pressure, diabetes, etc., which will cause coronary heart disease, myocardial i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06A61P1/16A61P37/02A61P3/04A61P7/00A23L33/135C12R1/225
CPCA61K35/747A23L33/135A61P1/16A61P3/04A61P3/06A61P7/00A61P37/02C12N1/20
Inventor 徐文艺李转羽赵柏闻魏然
Owner BEIJING QUANTIHEALTH TECH CO LTD
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