Supercharge Your Innovation With Domain-Expert AI Agents!

Method for detecting selenomethionine in selenium-enriched fish

The technology of selenomethionine and detection method is applied in the field of detection of selenomethionine in selenium-enriched fish, and achieves the effects of high sensitivity and good selectivity

Pending Publication Date: 2021-08-31
SHAANXI UNIV OF SCI & TECH +1
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, selenium-enriched fish is a better choice for people to choose selenium-enriched food, but the application of HPLC-ESI-MS technology to the detection of selenomethionine in selenium-enriched fish has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting selenomethionine in selenium-enriched fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0053] The detection method of selenomethionine in a kind of natural selenium-enriched fish of the present invention:

[0054] 1. Preparation of selenomethionine standard stock solution

[0055] Accurately weigh 10 mg of selenomethionine standard substance into a 10 mL brown volumetric flask, add water to dissolve, add acetonitrile to make up to the mark, shake well, and use it as a standard stock solution.

[0056] 2. Limit of detection and limit of quantitation

[0057] Take the selenomethionine standard stock solution, dilute it step by step with the blank sample extract, configure a series of standard solutions matching the matrix, the concentration range is 0-150 μg / L, and perform detection according to the above method. The matrix matching standard curve was drawn with the chromatographic peak area (Y) as the ordinate and the concentration (X) as the abscissa, and the detection limit of the method was calculated with the signal-to-noise ratio S / N≥3 and S / N≥10 (English n...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for detecting selenomethionine in selenium-enriched fish. The method comprises the following steps: mashing the selenium-enriched fish, and dissolving a fish meat sample with a Tris-HCl buffer solution; sequentially adding trypsin and protease K into the obtained mixed solution for enzymolysis; centrifuging the obtained enzymatic hydrolysate, adding acetonitrile into supernate, standing to obtain supernate, and filtering the supernate to obtain liquid to be detected; separating selenomethionine in the series of standard solutions, detecting, and drawing a matrix matching standard curve; separating and then detecting selenomethionine in a liquid to be detected by using high performance liquid chromatography, extracting a chromatographic peak according to an ion pair formed by parent ions and daughter ions, and obtaining the area of the chromatographic peak through integration; and substituting the chromatographic peak area obtained from the to-be-detected liquid into the matrix matching standard curve, and obtaining the concentration of selenomethionine in the selenium-enriched fish according to the volume of the supernatant and the mass of the fish meat sample.

Description

technical field [0001] The invention relates to the technical field of detection of selenomethionine, in particular to a detection method of selenomethionine in selenium-enriched fish. Background technique [0002] Selenium is an essential micronutrient that plays a key role in several major metabolic pathways, including thyroid hormone metabolism, antioxidant defense system, and immune function. Selenium deficiency has been linked to a range of serious diseases such as cancer, cardiovascular and inflammatory diseases, as well as other free radical-related problems such as premature aging. The human requirement for selenium is mainly obtained through diet, which includes inorganic forms (selenate and selenite) and organic forms (mainly selenomethionine and selenocysteine). Although selenium plays an important role in human health, long-term oral intake of excessive selenium can lead to selenium poisoning. Whether it is organic selenium or inorganic selenium, it is safe to u...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14G01N30/86G01N30/34G01N30/04
CPCG01N30/02G01N30/72G01N30/06G01N30/14G01N30/8634G01N30/34G01N30/04G01N2030/067G01N2030/146G01N2030/047
Inventor 贾玮马如甜夏曾润石琳祁蒙
Owner SHAANXI UNIV OF SCI & TECH
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com