Stem grafting method for melon crop stock-scion identification signal research

Abstract

The invention discloses a stem grafting method for melon crop stock-scion identification signal research, and belongs to the technical field of agricultural planting. The grafting method comprises the following steps: cultivating a melon seedling A and a melon seedling B; beveling the melon seedling A to obtain a scion with a cut, cutting a stem of the melon seedling B from a plant to obtain an in-vitro stock with the cut, and grafting the scion and the stock to obtain a grafted stem segment; performing healing culture in a healing room; sampling a grafted stem segment interface, a non-grafted scion A and a non-grafted stock B, respectively carrying out transcriptome sequencing to collect differential genes of the three, and screening the melon stock-scion early identification signal gene through union analysis. According to the method disclosed by the invention, the influence of scion leaves and rootstock root systems on grafting interface signals is eliminated, only stem segments are reserved, and grafting among tissues is performed, so that the gene screening range is favorably reduced, early signal identification key genes are screened out, and a technical support is provided for research on grafting incompatibility of melon crops.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a stem segment grafting method used for the research on rootstock and ear recognition signals of melon crops. Background technique [0002] my country is a big producer and consumer of melon crops such as melons, watermelons and cucumbers, and occupies a very important position in the cultivation of melon crops in the world. However, long-term fixed land cultivation has resulted in serious soil continuous cropping obstacles and the spread of soil-borne diseases, seriously threatening the development of the industry. As an effective solution, grafting has been widely used in the production of melon crops such as melons. [0003] Grafting, as a kind of asexual reproduction technology of plants, has a history of 3,000 years of application, and is now widely used in the production of vegetables, fruit trees and flowers. Grafting technology can improve the ...

AI Technical Summary

Problems solved by technology

Therefore, graft affinity is an important barrier limiting the use of grafting techniques

[0005] In the prior art, Yemon and Brown (1976) proposed that there is a heterologous recognition mechanism involving cell wall polymers between rootstock cells at the grafting interface of plants; Cookson et al. (2014) analyzed the combined healing of grape autologous and heterologous grafting Gene expression at the grafting interface at different time points during the period, 1105 candidate genes related to grafting affinity were found, and the up-regulation of genes related to peroxide and disease course was found. It is believed that there is an immune recognition mechanism that affects grafting affinity; tobacco The grafting of 73 species of angiosperms from 38 families is mainly due to the fact that the β-1,4-glucanase gene (GH9B3) promotes the formation of functional tissue adhesion and promotes the co-existence between scion and rootstock. plastid transport, which solves the problem of accumulation of necrotic layers in distant grafts (Notaguchi et al 2020), however, in the tobacco family intergrafting system, where there is less reconnection of xylem and almost no reconnection of phloem, tobacco grafting does not resolve plant The problem of grafting incompatibility is that it can adhere to a variety of plants and survive, but the growth is not normal

It can be seen that the problem of low grafting affinity has not been solved in the prior art, and most of the studies on grafting affinity in the prior art use intact plants, lacking the mechanism of early signal recognition and grafting affinity in a highly controlled environment. Therefore, it is impossible to effectively solve the problem of low grafting affinity, and how to screen the early recognition signal of grafting to solve the problem of low grafting affinity has become a difficult problem to be solved by those skilled in the art.

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