Combined control method for strawberry fusarium wilt

Abstract

The invention discloses a combined control method for strawberry fusarium wilt. The method comprises the following specific steps: firstly, culturing trichoderma obtained by separating and purifying petiole parts of diseased strawberry plants in a PDA culture medium, and placing in a refrigerator at 4 DEG C for later use after hyphae overgrow on a flat plate; then diluting ALA to 3000 times with clear water, and spraying the diluted ALA on the leaf surfaces of strawberries until liquid drops drip; and after 7 days, taking fresh cultured trichoderma blocks, inversely buckling the trichoderma blocks on the basal parts of the stems of the strawberries, and enabling the trichoderma blocks to be in full contact with the petioles and the soil. After the ALA and the trichoderma jointly act on the diseased plants, the growth of the trichoderma can be promoted, the growth of fusarium oxysporum and aspergillus fumigatus is inhibited, the inhibition effect of the trichoderma on the fusarium oxysporum is remarkably enhanced, the pathogenicity of the fusarium oxysporum is reduced, and the strawberry fusarium wilt can be effectively controlled.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a method for preventing and treating strawberry wilt by combining Trichoderma and ALA. Background technique [0002] Strawberry (Fragaria×ananassa Duch.) is a perennial herbaceous plant of the Rosaceae Fragaria genus, and its output ranks first in the production of small berries in the world. Not only has a sweet taste, but also rich in nutrients, and has the reputation of "Queen of Fruits". However, due to the limitation of the amount of cultivated land in our country and the frequent use of protected cultivation in production, the continuous cropping system not only reduces soil fertility, but also easily breeds diseases and insect pests. Strawberry wilt is one of the most important diseases, and even occurs in major strawberry planting areas around the world. It is increasing year by year, seriously restricting the sustainable development of strawberry ...

AI Technical Summary

Problems solved by technology

Therefore, chemical methods are not sustainable in the long run as they pollute the atmosphere, damage the environment, leave harmful residues and, with repeated use, can lead to the development of resistant strains in target organisms, reducing or eliminating synthetic pesticides in agriculture application is extremely necessary

[0005] Biological control has attracted much attention in recent years because of its low cost, high efficiency, environmental friendliness, and no drug residues. There are also many studies on biocontrol fungi to deal with Fusarium wilt. Restrictions, including environmental factors such as light, temperature, moisture, soil pH, and nutrients, are also easily affected by other microorganisms in the soil, resulting in unsatisfactory biocontrol effects. Most biocontrol bacteria can only stay in experimental research, and only a few A few are actually produced commercially

In the first issue of 2019, Biological Disaster Science Impurities published a study on the control of strawberry seedling wilt by compound Trichoderma preparations. It mainly uses Trichoderma for biological control, but requires multiple Trichoderma strains combined with Jingjia·hymexazol It is used as a wettable powder, although it has a certain control effect, but the treatment process is more complicated, and the use of Jingjia·hymexazol will cause pesticide residues, environmental protection is not enough, and it is easy to cause secondary pollution.

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