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The application of enols in pine needles to regulate the microbial community structure of Panax notoginseng rhizosphere

A technology of microbial community and middle enol, which can be applied in the fields of application, plant protection, fertilizer mixture, etc., and can solve problems such as continuous cropping obstacles of Panax notoginseng

Active Publication Date: 2022-02-01
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In order to solve the problem of Panax notoginseng continuous cropping obstacles, improve the planting and growth environment of Panax notoginseng, and improve the quality of Panax notoginseng, the invention provides a kind of enol substances in pine needles that can regulate the structure of the microbial community of Panax notoginseng rhizosphere and / or repair the continuous cropping of Panax notoginseng Carrier and / or application in improving Panax notoginseng planting

Method used

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  • The application of enols in pine needles to regulate the microbial community structure of Panax notoginseng rhizosphere
  • The application of enols in pine needles to regulate the microbial community structure of Panax notoginseng rhizosphere
  • The application of enols in pine needles to regulate the microbial community structure of Panax notoginseng rhizosphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1: α-pinene regulation of Sanqi rhizosphere microbial community structure test

[0065] In this experiment, the fumigation method in a closed space was adopted: the potted notoginseng plants after transplanting and surviving were placed in a transparent plastic airtight incubator, and 0 and 5 μL / L α-pinene was added to each box (volume of α-pinene / volume of incubator). The fumigation time was 3 days. Afterwards, the rhizosphere soil of potted Panax notoginseng was collected, passed through a 60-mesh sieve, and the change of soil microorganisms was measured by the smear plate method. Bacterial culture was carried out on beef extract peptone medium, and fungal culture was carried out on Bengal red medium. Use an electronic balance to accurately weigh 5g of the sieved and mixed soil sample, pour it into a 50mL centrifuge tube filled with 45mL of sterile water, place it on a shaker (120r / min, room temperature), and shake for 20min to fully disperse the soil into a so...

Embodiment 2

[0067] Example 2: D-pinitol and 2,3-butanediol regulate the microbial community structure of Panax notoginseng rhizosphere

[0068] This test adopts the method of spraying in a confined space: place the potted plants of Panax notoginseng after transplanting and surviving in a transparent plastic sealed incubator, with 10 plants of Panax notoginseng in each pot, 6 pots in each incubator, and spray 100mL of Panax notoginseng leaves three different treatments of sterile water, 1mg / L D-pinitol and 1mg / L 2,3-butanediol, spraying sterile water as a control treatment, spraying once a day for 5 days, and then collecting three potted plants The rhizosphere soil of No. 7 was sieved through a 60-mesh sieve to measure the change of soil microorganisms by the smear plate method. The beef extract peptone medium was used for bacterial culture, and Bengal red medium was used for fungal culture. Use an electronic balance to accurately weigh 5g of the sieved and mixed soil sample, pour it into ...

Embodiment 3

[0070] Example 3: 2,3-butanediol regulates soil microbial community structure test in continuous cropping soil

[0071] In this experiment, the method of long-term watering with potted continuous cropping soil was adopted: the notoginseng continuous cropping soil was taken from the farmland of Wenshan notoginseng, and the retrieved continuous cropping soil was dried in the shade, then passed through a 40-mesh sieve to remove plant residues in the soil, and weighed 4 Add 1kg of continuous cropping soil to the finishing box (28×20×17cm) for later use; take 1mL of analytically pure 2,3-butanediol and add it to 1L of sterile water to prepare 1000ppm mother liquor, and then gradually dilute to 1, 10, 50 , 100ppm solution, and sterile water as a control treatment; take 500mL of the above solution and add it to different finishing boxes, then cover the lid of the finishing box, and seal it with a parafilm, and place the finishing box in a cool place away from light. Set up 3 repetiti...

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Abstract

The invention relates to the application of enol substances in pine needles in regulating the rhizosphere microbial community structure of Panax notoginseng and / or repairing the continuous cropping carrier of Panax notoginseng and / or improving the planting of Panax notoginseng. The present invention found that by using α-pinene, 2,3-butanediol and D-pinitol, which are rich in pine needles, to treat potted notoginseng or soil by fumigation, spraying or watering, it is possible to reduce the The number of fungi in the actual soil or the continuous cropping soil of Panax notoginseng, and significantly increase the number of bacteria, so that the ratio of fungi / bacteria in the soil is significantly reduced, and the soil is changed from "fungus type" to "bacteria type", which is beneficial to the planting of Panax notoginseng and grow.

Description

technical field [0001] The invention relates to the technical field of notoginseng planting, in particular, the invention relates to the application of enol substances in pine needles in regulating the structure of the notoginseng rhizosphere microbial community. Background technique [0002] Panax notoginseng (Burk.) F.H.Chen, also known as Tianqi and Jinbuhuan, is a perennial herbaceous plant of the genus Araliaceae. Panax notoginseng has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. It has been favored by people since ancient times, and has a history of domestication and cultivation for more than 400 years. At present, notoginseng production areas are concentrated in Wenshan, Yanshan, Maguan, Guangnan, Qiubei, Luxi, Maitreya, Gejiu, Mengzi, Jianshui, Shiping, Pingbian, Shilin in Yunnan Province, and Jingjing in Guangxi Zhuang Autonomous Region. In counties and cities such as Xixi, the annual output of Panax notoginseng e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/14A01B79/02A01G13/00A01G22/25
CPCC09K17/14A01G13/00A01B79/02A01G22/25
Inventor 朱书生张俊星叶辰李天尧黄惠川刘屹湘杨敏梅馨月邓维萍何霞红朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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