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Method for evaluating organic matter degradation potential of biological filter material

A bio-filter and microbial degradation technology, which is applied in the field of evaluating the potential of bio-filter to degrade organic matter, can solve the problems of long evaluation period, easy pollution, inconvenient operation, etc.

Active Publication Date: 2021-09-07
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved by the present invention is to overcome the defects of long evaluation cycle, inconvenient operation, and easy pollution of the method for evaluating the potential of biological filter materials to degrade organic matter in the prior art, thereby providing a method for evaluating biological filter materials based on metagenomic sequencing. Methods of degrading the potential of organic matter

Method used

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  • Method for evaluating organic matter degradation potential of biological filter material
  • Method for evaluating organic matter degradation potential of biological filter material
  • Method for evaluating organic matter degradation potential of biological filter material

Examples

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Embodiment 1

[0048] This embodiment provides a method for evaluating the potential of biological filter material to degrade organic matter. The target organic matter evaluated is atrazine, atenolol, sulfadiazine and carbamazepine respectively. The steps are as follows:

[0049] (1) Establish a reference database: determine the degradation genes and / or degradative enzymes (as shown in Table 1) of the microorganisms to the target organic matter according to the literature and the enviPath database; if the degradation genes can be found, obtain them in the Genbank database and / or the KEGG database For the DNA sequence of the degrading gene, if the degrading enzyme can be found, the protein sequence of the degrading enzyme is obtained in the Swiss-Prot database.

[0050] Table 1 Degradation genes, degrading enzymes and degradation pathways of target organic substances

[0051]

[0052]

[0053] The references in Table 1 are as follows:

[0054] [1]. De Souza, M.L.; Sadowsky, M.J.; Wacke...

Embodiment 2

[0081] The present embodiment provides a method to verify the reliability of the method for evaluating the potential of biological filter material to degrade organic matter in Example 1, the steps are as follows:

[0082] (1) Preparation of organic solution

[0083] Atenolol (Atenolol, C 14 h 22 N 2 o 3 ) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.; Atrazine (Atrazine, C 8 h 14 ClN 5 ), sulfadiazine (Sulfadiazine, C 10 h 10 N 4 o 2 S) and carbamazepine (Carbamazepine, C 15 h 12 N 2 O) purchased from Shanghai McLean Biochemical Technology Co., Ltd.; Atenolol acid (Atenololacid, C 14 h 21 NO 4 ), Hydroxyatrazine (Hydroxyatrazine, C 8 h 15 N 5 O), deethylatrazine (Deethylatrazine, C 6 h 10 ClN 5 ), deisopropyl atrazine (Deisopropylatrazine, C 5 h 8 ClN 5 ), Carbamazepine 10,11-epoxide (Carbamazepine-10,11-epoxide, C 15 h 12 N 2 o 2 ), iminostilbene (Iminostilbene, C 14 h 11 N), 2-aminopyrimidine (2-Aminopyrimidine, C 4 h 5 ...

Embodiment 3

[0099] In this embodiment, the abundance of degrading genes and / or degrading enzymes is used to evaluate the potential of the biological filter material to degrade organic matter. The steps are as follows:

[0100] (1) Sampling

[0101] Sampling was carried out in quick sand filter pools of 9 drinking water plants nationwide, and the sampling method was referred to in Example 1.

[0102] (2) DNA extraction and metagenomic sequencing

[0103] Refer to Example 1 for the methods of DNA extraction and metagenomic sequencing.

[0104] (3) Calculate the abundance of degraded genes

[0105] Use Usearch v10.0.240 (-ublast-accel 0.8) to compare the DNA sequence in the metagenomic sequencing data obtained with the DNA sequence of the degraded gene in the reference database established in Example 1, to determine the microorganisms in the biofilter sample. Contains degradation genes;

[0106] Using the DNA sequence of the degraded gene as the reference sequence, add the length of the ...

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Abstract

The invention provides a method for evaluating organic matter degradation potential of a biological filter material, and relates to the field of drinking water treatment. The method comprises the following steps: establishing a reference database of microbial degradation target organic matters; carrying out DNA extraction on the biological filter material sample and obtaining metagenome sequencing data; sequentially carrying out genome assembly and binning, screening and redundancy removal on the metagenome sequencing data to obtain a non-redundant MAGs set; and performing species annotation, calculating microbial abundance, comparing a non-redundant MAGs set with the established database, determining MAGs containing degrading genes and / or degrading enzymes, obtaining the corresponding microbial abundance, and evaluating the potential of the biological filter material for degrading organic matters by using the obtained microbial abundance. According to the method, only metagenome sequencing and analysis are carried out on the filter material sample, organic matters do not need to be put into the filter for operation, the organic matters or degradation intermediate products do not need to be detected, the evaluation period is short, the operation is simple and convenient, and the environmental pollution is reduced.

Description

technical field [0001] The invention relates to the technical field of drinking water treatment, in particular to a method for evaluating the potential of a biological filter material to degrade organic matter. Background technique [0002] Human industrial production activities and domestic sewage discharge have led to a large amount of organic matter being discharged into the receiving water environment, especially some organic matter can often be detected in drinking water sources. Filter water is currently the only way to degrade organic matter in water bodies. an important means. The filter has the function of physical filtration, the pores of the filter material can effectively retain microorganisms and some chemical pollutants in the source water, and the adsorption capacity of the filter material itself can effectively promote the removal of pollutants, while the retained microorganisms are oligotrophic and intermittent Under the condition of hydraulic impact, it ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/30G16B30/10G16B30/20G16B35/20G16B50/00
CPCG16B20/30G16B30/20G16B35/20G16B30/10G16B50/00
Inventor 柏耀辉周洁王东麟林慧曲久辉
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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