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Method for preparing chitosan and graft copolymer from squid fan-shaped bones

A technology of graft copolymer and chitosan, which is applied in the field of preparation of chitin polysaccharide, can solve problems such as difficult removal, inability to exert the hemostatic function of chitosan, uneven dispersion, etc.

Pending Publication Date: 2021-09-10
荣成万盈水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chitosan hemostatic materials are mainly prepared by mixing chitosan with anti-inflammatory and analgesic drugs, which cannot exert the hemostatic function of chitosan, and after use, it is dispersed unevenly, adheres to the wound, and is not easy to remove, such as patents 200810198164.4 and 201310662052.0

Method used

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  • Method for preparing chitosan and graft copolymer from squid fan-shaped bones

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of squid fan-shaped bone prepares the method for chitosan and graft copolymer, it passes through the following processing steps:

[0039] 1) Degradation of chitin

[0040] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0041] b. Add hydrogen peroxide to the squid fan-shaped bone meal obtained in step a, and rapidly heat up to 130°C hydrogen peroxide detonation point, and the treatment is completed to obtain oxidized chitosan; wherein, the concentration of the hydrogen peroxide is 68%, and the squid fan is soaked in hydrogen peroxide before heating bone meal 2.5h,

[0042] 2) Enzymolysis

[0043] Adding the oxidized chitosan obtained in step b to a composite protease, enzymatically hydrolyzing it for 10 h under the action of ultrasonic waves, centrifuging and taking the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline prote...

Embodiment 2

[0051] A kind of squid fan-shaped bone prepares the method for chitosan and graft copolymer, it passes through the following processing steps:

[0052] 1) Degradation of chitin

[0053] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0054]b. Add hydrogen peroxide to the squid fan-shaped bone meal obtained in step a, and rapidly heat up to 100°C hydrogen peroxide detonation point, and the treatment is completed to obtain oxidized chitosan; wherein, the concentration of the hydrogen peroxide is 60%, and the squid is soaked in hydrogen peroxide before heating Fan-shaped bone meal 2h,

[0055] 2) Enzymolysis

[0056] Adding the oxidized chitosan obtained in step b to a composite protease, enzymatically hydrolyzing it for 10 h under the action of ultrasonic waves, centrifuging and taking the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline p...

Embodiment 3

[0064] A kind of squid fan-shaped bone prepares the method for chitosan and graft copolymer, it passes through the following processing steps:

[0065] 1) Degradation of chitin

[0066] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0067] b. Add hydrogen peroxide to the squid fan-shaped bone meal obtained in step a, and rapidly heat up to 150°C hydrogen peroxide detonation point, and the treatment is completed to obtain oxidized chitosan; wherein, the concentration of the hydrogen peroxide is 70%, and the squid fan is soaked in hydrogen peroxide before heating bone meal 3h,

[0068] 2) Enzymolysis

[0069] Adding the oxidized chitosan obtained in step b to a composite protease, enzymatically hydrolyzing it for 12 hours under the action of ultrasonic waves, centrifuging and taking the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline pro...

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Abstract

The invention discloses a method for preparing chitosan and a graft copolymer from squid fan-shaped bones. The method comprises the following steps: 1) degrading chitin; 2) performing enzymolysis; 3) purifying; 4) synthesizing a copolymer; and 5) synthesizing a copolymer grafted compound. The method comprises the steps: by taking the squid fan-shaped bones as a raw material, soaking the squid fan-shaped bones in hydrogen peroxide, performing oxidative degradation on the squid fan-shaped bones by utilizing the explosion concentration of hydrogen peroxide and using a controllable explosion technology to obtain degraded chitin, adding compound enzyme, further decomposing the oxidative degradation chitin, removing impurities through carbon dioxide supercritical fluid, and carrying out copolymer preparation and grafting reaction on the obtained chitosan to finally obtain the grafted copolymer of the chitosan. The hydrogen peroxide blasting combined enzyme method is adopted, the prepared chitosan is large in molecular weight, the product copolymerization grafting effect is good, the technological process is simple, efficient, green and free of pollution, and large-scale application and popularization are facilitated.

Description

technical field [0001] The invention relates to the preparation of chitin polysaccharide, in particular to a method for preparing chitosan and graft copolymer from squid fan-shaped bone. Background technique [0002] As we all know, squid fan-shaped bones, shrimp heads and shrimp shells and other aquatic leftovers are rich in nutrients and rich in chitin, and the chitin content is 3-5%. Except for recovered protein, most of them are directly dried and used as feed, or even discarded as garbage. [0003] Squid fan-shaped bone can prepare chitosan, its chitosan has unique biological properties, including biocompatibility, biodegradability and non-toxicity, it is a material for wound treatment application, chitosan has antibacterial, hemostatic and adhesive properties , but insoluble in low pH environment is still the main obstacle in its application. There are reactive groups such as hydroxyl and amino groups on the chitosan molecular chain, so chitosan can be modified by ch...

Claims

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Application Information

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IPC IPC(8): C08F251/00C08F220/06C08B37/08
CPCC08F251/00C08B37/003C08B37/0003C08F220/06Y02P20/54
Inventor 吴文惠杨劲峰毕进红
Owner 荣成万盈水产科技有限公司
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