Fluorescence sensing system, preparation method and application thereof

A fluorescence sensing and nano-fluorescence technology, applied in the field of fluorescence sensing system and its preparation, can solve the problems of complex operation, long time, expensive instruments, etc., and achieve the effects of improving sensitivity, low reagent consumption and fast reaction time

Active Publication Date: 2022-08-02
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] In recent years, the detection methods of patulin mainly include gas phase / liquid chromatography, gas phase / liquid chromatography tandem mass spectrometry, enzyme-linked immunoassay and thin layer chromatography, etc. These detection methods have high sensitivity and a large amount of sample detection. Also well suited for the separation and analysis of complex samples; however, most of them require expensive instrumentation, complicated operations, and lengthy time periods, which limits their widespread use in field testing, especially in resource-scarce regions

Method used

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  • Fluorescence sensing system, preparation method and application thereof
  • Fluorescence sensing system, preparation method and application thereof
  • Fluorescence sensing system, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Thiol-modified nano-fluorescent liposomes (CM6@Lip-SH) coated with coumarin 6 and amino-functionalized gold-coated ferric oxide nanoparticles (NH 2 -Au@Fe 3 O 4 ) preparation:

[0046] 1. Preparation of thiol-modified nano-fluorescent liposomes (CM6@Lip-SH) coated with coumarin 6, the specific steps are as follows:

[0047] (1) Dissolve 20 mg of cholesterol, 100 mg of soybean lecithin, 5 mg of DSPE-PEG-SH, and 0.1 mg of coumarin 6 in 15 mL of dichloromethane, and sonicate until uniformly dispersed. The dispersion was transferred to a round-bottom flask and placed in a rotary evaporator to completely evaporate the solvent, forming a thin film attached to the bottle wall, and adding 2mL of 0.01mol L to the round-bottom flask -1 PBS buffer solution (pH=7.4) and 50 μL TCEP (10 mmol L -1 ) solution, continued to hydrate for 1 hour, and finally a green gelatinous liquid was obtained.

[0048] (2) The gelatinous liquid obtained in (1) was sonicated for 3 min under a cell ...

Embodiment 2

[0059] The patulin fluorescence sensing system prepared in Example 1 measures the patulin in the sample

[0060] All fluorescence measurements were performed on an F-7000 spectrophotometer (Hitachi, Japan) with an excitation wavelength of 455 nm and an emission wavelength of 505 nm. The slit width for excitation and emission is 10 nm. Scan speed is 1200nm min -1 , the response time is 0.5s. The photomultiplier tube (PMT) voltage is 400V.

[0061] All fluorescence measurements were performed on an F-7000 spectrophotometer (Hitachi, Japan) at an excitation wavelength of 455 nm and an emission wavelength of 505 nm when collecting fluorescence intensity signals; the slit width for excitation and emission was 10 nm; scanning Speed ​​is 1200nm min -1 , the response time is 0.5s; the photomultiplier tube (PMT) voltage is 400V;

[0062] Specific detection steps:

[0063] (1) Add 200 μL of coumarin-6-coated thiol-modified nano-fluorescent liposome dispersion dropwise to 50 μL of ...

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Abstract

The invention provides a fluorescence sensing system, which is composed of a thiol-modified nano-fluorescent liposome CM6@Lip-SH dispersion liquid coated with coumarin 6 and an amino-functionalized gold-coated ferric oxide nanoparticle NH 2 ‑Au@Fe 3 O 4 Composition of the dispersion. The fluorescence sensing system provided by the invention can be used for the rapid detection of patulin, and has good selectivity, high recovery rate, little background interference and good reproducibility; it does not need to rely on expensive large-scale instruments, the pretreatment is simple, and the accuracy of the results is guaranteed. At the same time, rapid detection is achieved, which greatly saves costs and improves detection efficiency.

Description

technical field [0001] The invention relates to the technical field of food detection and analysis, in particular to a fluorescence sensing system and a preparation method and application thereof. Background technique [0002] Patulin (PAT) is a highly dangerous mycotoxin produced by Aspergillus, Penicillium, and Silky chlamydia frequently found in fruits, especially apples, grapes and their derivatives middle. Acute and chronic ingestion of patulin can cause respiratory and urinary system damage, nerve paralysis, pulmonary edema, and renal failure in humans. Due to the high toxicity and widespread availability of patulin, the US, EU and China have established a maximum limit of 50 ng mL in apple juice -1 . [0003] In recent years, the detection methods of patulin mainly include gas / liquid chromatography, gas / liquid chromatography tandem mass spectrometry, enzyme-linked immunoassay and thin-layer chromatography, etc. These detection methods have high sensitivity and larg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C09K11/06
CPCG01N21/64G01N21/6428C09K11/06C09K11/025G01N2021/6417C09K2211/1088
Inventor 韩铮聂冬霞赵志辉郭大凯黄晴雯
Owner SHANGHAI ACAD OF AGRI SCI
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