Multifunctional hydrogel for promoting blood circulation long-acting opening and preparation method thereof

A multifunctional, hydrogel technology for capsule delivery, pharmaceutical formulations, nanocapsules, etc.

Inactive Publication Date: 2021-09-14
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Multifunctional hydrogel for promoting blood circulation long-acting opening and preparation method thereof
  • Multifunctional hydrogel for promoting blood circulation long-acting opening and preparation method thereof
  • Multifunctional hydrogel for promoting blood circulation long-acting opening and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1: the preparation of the chitosan (CHI-C) of catechol modification

[0032] Add 2g chitosan and 250mL, 0.5mol / L HCl into a 500mL Erlenmeyer flask, fully dissolve and adjust the pH of the solution to 4.5 with 0.1g / mL NaOH to obtain solution A. Take 1.89g catechol and dissolve it in 10mL deionized water, add it into solution A after dissolving to obtain solution B. Get 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride 1.24g and dissolve it in a mixed solution of 40mL water and ethanol (the volume ratio of water and ethanol is 1:1), and Slowly drop it into solution B and use 1mol / L NaOH to adjust the pH of the solution to 4.5. After reacting for 5 hours, pour the reaction solution into a dialysis bag (cellulose, with a molecular weight cut-off of 12000Da), dialyze with a hydrochloric acid solution of pH=3 for three days, and then Dialysis in deionized water for 7 hours, the dialysate was frozen in liquid nitrogen, freeze-dried and stored at -80°C to ...

Embodiment 2

[0033] Embodiment 2: the preparation of the dextran (ODex) of aldylation modification

[0034] Dissolve 2g of dextran in 200mL of deionized water and stir to dissolve fully to obtain solution C; dissolve 3g of sodium periodate in 60mL of deionized water to obtain solution D; add solution D to solution C, and react in the dark for 24 hours during the whole process Pour it into a dialysis bag (cellulose, molecular weight cut-off 3500Da), dialyze with deionized water for 3 days, freeze the dialysate in liquid nitrogen, freeze-dry it and store it at -80°C to obtain formylated dextran.

Embodiment 3

[0035] Embodiment 3: the preparation of nano gelatin microsphere (nps)

[0036] Dissolve 1.5g of pigskin gelatin in 30mL of deionized water, add 30mL of acetone to it after completely dissolving, then discard the upper liquid after standing for a while, and then dissolve it again with 30mL of deionized water to obtain solution E. Then the pH value of solution E was adjusted to 2 with 1mol / L hydrochloric acid, 75mL acetone was slowly added dropwise thereto, and 750 μL glutaraldehyde aqueous solution (mass fraction was 25%) was added thereto after the dropwise addition, to obtain solution F. After reacting for 16 hours, the solution F was taken out and centrifuged, and the centrifuged product was washed with water three times and then fully freeze-dried to obtain nano-gelatin microspheres (nps), which were stored at -80°C.

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Abstract

The invention provides multifunctional hydrogel for promoting blood circulation long-acting opening after an ultra-long flap transplantation operation and a preparation method thereof, and belongs to the technical field of multifunctional hydrogel. Natural polysaccharide and gelatin are used as raw materials, and the raw materials are simply modified to have various biological functions. The preparation method specifically comprises the following steps of: doping nano gelatin microspheres into the gel, then directly doping CORM into the gel, and then loading GSNO into the nano gelatin microspheres to construct the multifunctional gel which has biological multifunctionality, can slowly release drug molecules in a gradient manner and can promote blood vessel opening for a long time. The biological raw materials are wide in source and low in price, and have no biological toxicity after being modified. The gel for promoting blood circulation long-acting opening is simple in administration mode, can be used for local intraoperative administration, has good adhesion hemostasis and antibacterial effects, and reduces the risk of intraoperative bleeding and postoperative infection; and two gas drug molecules can be locally and slowly released, thereby avoiding excessive dosage circulating toxicity caused by intravenous administration.

Description

technical field [0001] The invention belongs to the technical field of multifunctional hydrogels, and in particular relates to a multifunctional hydrogel for promoting long-term opening of blood supply and a preparation method thereof. Background technique [0002] Skin flap transplantation, as the most important means of severe wound defect and plastic repair and reconstruction in clinical work, has been plagued by insufficient blood supply and distal necrosis, and the degree of openness of blood supply directly determines the survival rate and final outcome of skin tissue transplantation. At the same time, the larger the repair area, the higher the risk of flap necrosis. Therefore, the development of an effective biomaterial that can promote the long-term opening and maintenance of blood supply after ultra-long flap transplantation to solve the complex repair needs in actual work has become the top priority of the current work. [0003] People have developed a variety of ...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/52A61L27/54A61L27/58A61L27/60C08J3/075C08L5/08C08L5/02C08L89/00
CPCA61L27/52A61L27/60A61L27/26A61L27/58A61L27/54C08J3/075A61L2300/624A61L2300/418A61L2300/404A61L2300/252A61L2300/224C08J2305/08C08J2305/02C08J2489/00C08L5/08C08L5/02C08L89/00
Inventor 路来金张俊虎任景炎唐晓铎杨柏
Owner JILIN UNIV
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