Biological culture micro-fluidic chip suitable for microgravity gyroscope assembly and cell culture method thereof

A microfluidic chip and biological culture technology, applied in the field of space medical engineering and biotechnology research, can solve the problems of filling the multi-hole plate and the multi-hole plate being unusable, etc.

Active Publication Date: 2021-09-14
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF11 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More importantly, in traditional cell culture methods, multi-well plates are often used to increase the biological repetition of experiments. However, multi-well plates cannot be used in microgravity gyroscopes because they cannot be filled with medium

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biological culture micro-fluidic chip suitable for microgravity gyroscope assembly and cell culture method thereof
  • Biological culture micro-fluidic chip suitable for microgravity gyroscope assembly and cell culture method thereof
  • Biological culture micro-fluidic chip suitable for microgravity gyroscope assembly and cell culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The present invention provides a gyroscope assembly microgravity applicable biological growth microfluidic chip, such as figure 1 It is shown, from the bottom plate 1 includes a lower stationary culture chip body 3, flexible film 2, the fixed plate 4, the flexible membrane completely covers the culture chip body by detachable fixing means biological growth microfluidic chip side fixed, biological culture microfluidic chip fixed to the fixing position microgravity gyroscope.

[0046]Soft film is a polydimethylsiloxane PDMS, polyethylene PE, polyvinyl chloride PVC, polyvinylidene chloride PVDC. The culture may be a biological target cells, microorganisms, tissue, eggs, larvae. In order to culture the chip body to continue the experiment, a fixing method using a fixing device is detachably fixed all known methods, such as using a fixed holder holding, fixing bolts. Produced by the fixing bolt plate of the chip body and the soft membrane pressure, so that deformation of soft fi...

Embodiment 2

[0048] like figure 2 , The culture chip body includes an interface layer 5, layer 6 and the substrate layer 7 culture, the culture the culture chamber layer aspect ratio of 0.003 to 1, the interface layer is provided with a plurality of through holes 8, the through-hole corresponding to the position of the culture chamber . Preferably, when loading, the culture media should not through the through hole of the chamber, to ensure that the culture medium was added to fill the entire chamber, no bubbles.

[0049] T12.5 filled with standard culture flasks need 37mL medium, the number of cell wall (cell) is completely attached to the intersection: medium required (mL) = 1.4x10 6 : 37, as compared therewith, use of figure 2 Chip shown culture experiments carried out, the bottom surface of the culture chamber to 12.5cm 2 , The culture chamber filled with culture medium need only 2.5mL, the number of cell wall (cell) is completely attached to the intersection: medium required (mL) = 1.4x10...

Embodiment 3

[0056] like image 3 As shown in this patent includes an interface culture chip body layer 5, layer 6 and the substrate layer 7 culture, the culture layer is provided with a plurality of culture chambers 9, each of the culture chamber has an inlet passage 10 and fluid passage 11, culture chamber aspect ratio of 0.003 to 1, the interface layer is provided with a plurality of through holes 8, the inlet passage and the through hole and the position of the liquid culture chamber corresponding to the channel. Conventional perforated plates commonly used in cell culture in vitro cell research requires small sample size, number of repetitions of biological experiments, however, the traditional cell culture multiwell plate can not be applied to simulate microgravity environment, using only culture bottles repeated experiments.

[0057] The detection of apoptotic cell viability test cells or flasks used in connection microgravity gyroscope requires six flasks, three experimental groups, thr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a biological culture micro-fluidic chip suitable for microgravity gyroscope assembly and a cell culture method thereof. A chip soft film completely covers a culture chip body, the side face of the biological culture micro-fluidic chip is fixed through a detachable fixing device, sealing is formed between the soft film and a culture chip interface layer, and the biological culture micro-fluidic chip is fixed to a fixing position of a microgravity gyroscope. The chip greatly reduces the depth-to-width ratio of a culture chamber, reduces the use amount of a culture medium, can meet the parallel culture requirement, and meanwhile effectively reduces the damage of fluid shear stress to a culture target.

Description

Technical field [0001] The present invention is in the field of space engineering, and medical research biotechnology, particularly to biological growth microfluidic chip assembly, and a cell culture method using the microfluidic chip is adapted to a microgravity gyroscope (Random Position Machine, RPM). Background technique [0002] Gravity space environment is generally zero to a few thousandths of G, ie microgravity environment. A variety of biological damage effects often occur in astronauts in orbit, involving the cardiovascular system, muscles, nervous system and the immune system. In order to explore the biological mechanisms of effect damage, protect the astronauts in orbit, the relevant research is necessary. However, the sample astronaut is extremely valuable, extremely limited opportunities in orbit to carry out biological experiments, so far the primary means of terrestrial biological research space is simulated microgravity. Wherein, for a cell sample in vitro, the m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/04C12M3/06C12M3/00C12M1/12C12M1/00B01L3/00A01K67/033
CPCC12M21/08C12M23/16C12M23/20C12M29/04C12M29/10C12M35/04C12M37/04B01L3/5027A01K67/033B01L2200/12B01L2300/0809B01L2300/0887
Inventor 马宏陈钰邓玉林吴语非麻陈灿王舒钥
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap