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Enzyme manufacture method, and enzyme

A manufacturing method and polymer technology, applied in biochemical equipment and methods, enzymes, hydrolytic enzymes, etc., can solve problems such as enzyme inactivation

Pending Publication Date: 2021-09-14
WILMAR SHANGHAI BIOTECH RES & DEV CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in practical applications, oils, especially fatty acids, are inevitably oxidized at high temperatures, and the produced peroxides inevitably inactivate enzymes
[0006] Therefore, there is still a lack of a reliable immobilization method to ensure that the enzyme is not inactivated during use, and further improvement is required for immobilized lipase.

Method used

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  • Enzyme manufacture method, and enzyme

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Put 10g of immobilized lipase Lipozyme TL IM granzyme (manufactured by Novozymes) and 35g AmberliteXAD16N resin (manufactured by Rohm and Haas) into a 250mL Erlenmeyer flask, then add 60mL of 0.05mol / L glycine buffer at pH8.0 solution, followed by adding 7g of glucose, and placed in an air bath shaker at 20°C for 10h. Then, the obtained mixture was put into a centrifuge and centrifuged at a centrifugal force of 8000 g for 3 min, the resin layer was taken out, and placed in a fume hood at room temperature for drying. After 24 hours, the moisture content was determined to be 8%.

Embodiment 2

[0101] Put 10 g of immobilized lipase Lipozyme TL IM granzyme (manufactured by Novozymes) and 20 g of Amberchrom CG1000 resin (manufactured by Rohm and Haas) into a 250 mL Erlenmeyer flask, then add 40 mL of 0.1 mol / L glycine buffer at pH 6.5 liquid, followed by adding 0.4g of maltose, placed in an air-bath shaker at 20°C and shaken for 10h. Then, the resulting mixture was put into a centrifuge and centrifuged at a centrifugal force of 6000 g for 3 min, the resin layer was taken out, and placed in a fume hood at room temperature for drying. After 24 hours, the moisture content was determined to be 7%.

Embodiment 3

[0103] 10g of immobilized lipase Lipozyme TL IM granzyme (manufactured by Novozymes) was cut into powder in a shearing machine, and then dropped into a 250mL conical flask with 90g NK110 resin (manufactured by Tianjin Nankai Hecheng Technology Co., Ltd.), Add 50 mL of 0.02 mol / L glycine buffer solution with pH 7.0, followed by 5 g of galactose, and shake in an air-bath shaker at 20°C for 10 h. Then, the resulting mixture was put into a centrifuge and centrifuged at a centrifugal force of 6000 g for 3 min, the resin layer was taken out, and placed in a fume hood at room temperature for drying. After 24 hours, the moisture content was determined to be 7%.

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Abstract

The invention provides an enzyme manufacture method, which comprises the following working procedures of: complex acquisition: enabling a solid enzyme and a high-molecular adsorbing material to be in contact under the condition that the pH value is 6-8 to obtain a solid enzyme-high-molecular adsorbing material complex; contact: enabling the solid enzyme-high-molecular adsorbing material complex to be in contact with a reducing sugar; and separation: carrying out separation from a mixed system obtained in the working procedure of the contact to obtain the enzyme, wherein the high-molecular adsorbing material comprises at least one of the materials selected from the group consisting of a non-polar macroporous adsorbent resin, a non-polar skeleton high-molecular polymer and a non-ionic hydrophobic cross-linked polymer. The enzyme manufacture method can effectively prevent enzyme activity from being reduced, provides the enzyme with high enzyme activity and excellent enzyme activity stability, prolongs the service life of the enzyme, has simple manufacture working procedures, is easy to control the process, can meet the requirements of high-efficiency and stable production, and effectively reduces the production cost of enterprises.

Description

technical field [0001] The invention relates to an enzyme production method, in particular to an enzyme production method capable of effectively preventing the reduction of enzyme activity and having stable enzyme activity. Background technique [0002] Lipase is a special class of acyl hydrolase, which has important applications in the oil industry. Enzymes are formulated on granules or powder carriers, which can be used for esterification, transesterification and other reactions of oils and fats. In the lipase immobilization process, the more common method is to use polymer adsorption resin or ion exchange resin as a carrier, and absorb lipase into its pores through the residual surface energy generated by its huge specific surface area, such as the US Patent Application No. 84306053.4 Immobilize the lipase produced by Rhizomucor miehei with ion-exchange resin with phenolic skeleton, and then use it for oil modification. In the actual operation process, the enzyme liquid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/10C12N11/04C12P7/64
CPCC12N11/08C12N11/10C12N11/04C12N9/20C12Y301/01003C12P7/6445
Inventor 辛本荣郑妍金倩茹
Owner WILMAR SHANGHAI BIOTECH RES & DEV CENT
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