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Preparation method of brucea javanica residue effective part containing picrasma quassioides lactone components

An effective part, the technology of Brucea javanica, applied in the field of preparation of the effective part of Brucea javanica dregs, to achieve the effects of increasing cell apoptosis rate, inhibiting tumor cell proliferation, reducing ascites volume and ascites cell viability

Pending Publication Date: 2021-09-17
GUANGZHOU BAIYUNSHAN MINGXING PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But, still do not have a kind of method of simply and effectively utilizing the dregs of Brucea javanica at present

Method used

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  • Preparation method of brucea javanica residue effective part containing picrasma quassioides lactone components
  • Preparation method of brucea javanica residue effective part containing picrasma quassioides lactone components
  • Preparation method of brucea javanica residue effective part containing picrasma quassioides lactone components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 Extraction of the effective part of bruce japonica dregs

[0050] 1. Materials

[0051] The dregs of Brucea javanica came from Guangzhou Baiyunshan Mingxing Pharmaceutical Co., Ltd. It was identified as Brucea javanica by Professor Su Ziren of Guangzhou University of Traditional Chinese Medicine, and the voucher samples were kept in Guangzhou University of Traditional Chinese Medicine.

[0052] 2. Experimental method

[0053] 1. Extraction of the effective parts of the dregs of bruce chinensis

[0054] 10kg of bruce japonica residues add 30 liters of 95% (V / V) ethanol heating and refluxing extraction for 2 times, each time for 2 hours, filter the filtrate with 8 layers of gauze, and concentrate the filtrate under reduced pressure and vacuum until there is no alcohol smell to obtain a brown sticky odor. Condensed total crude extract, add pure water, fully vibrate, suspend and shake to disperse the total crude extract, successively extract with petroleum et...

Embodiment 2

[0062] Embodiment 2 Purification, separation and identification of active ingredients in the effective parts of Brux japonica dregs

[0063] 1. Materials

[0064] Same as Example 1.

[0065] 2. Experimental method

[0066] Take the effective part of the drug residues of brucei chinensis described in Example 1, mix the samples with 100-200 mesh silica gel, and perform 200-300 mesh silica gel column chromatography with dichloromethane-methanol as the mobile phase (100:0-100: 20) Gradient elution, recrystallization from the fractions obtained by elution at 100:1 to obtain compound 1 and compound 2. After further purification by Sephadex LH20 (methanol) gel chromatography column chromatography, monomer components with higher purity were obtained. The structure was identified and analyzed by nuclear magnetic resonance and mass spectrometry.

[0067] 3. Experimental results

[0068] Among them, the purity of compound 1 was more than 98% detected by HPLC peak area normalization ...

Embodiment 3

[0072] Example 3 The anti-colonoma cell effect of the active ingredients of bruce chinensis dregs

[0073] 1. Experimental method

[0074] 1. Preparation of brucetinol and brucetin D working solution

[0075] 13.69 mg of brucei alcohol powder was weighed, and 2 mL of DMSO solution was added to prepare a 13.1635 mM stock solution of brucei alcohol. After it is fully dissolved, it is divided into 1.5mL centrifuge tubes and wrapped with parafilm. Before use, take 1998μL of sterilized complete medium and add it to the aliquoted 2μL stock solution, dilute 1000 times to 1.316×10 -2 mM test initial concentration. Take 200 μL of the initial concentration solution, add 1800 μL of complete medium, and dilute 10 times. Each time, according to the above dilution method, the brucei alcohol was diluted to the working solution concentration to ensure the repeatability of manual operation. Mark the date of preparation and store at -20°C.

[0076] 11.92 mg of corpusine D powder was weigh...

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Abstract

The invention discloses a preparation method of a brucea javanica residue effective part containing picrasma quassioides lactone components. The brucea javanica residue effective part prepared by the method contains 50% or more of the picrasma quassioides lactone components, and has exact effects of resisting ascites tumor, pancreatic cancer, lung cancer, triple negative breast cancer, and colorectal cancer. The effective part has the effects of inhibiting tumor cell proliferation and promoting tumor cell apoptosis on various tumor cells such as pancreatic cancer cells, non-small-cell lung cancer cells and colon cancer cells; the activity of the tumor cells can be remarkably reduced, tumor cell apoptosis is promoted, and the cell apoptosis rate is increased; the survival time of ascites tumor mice can be prolonged, increase of the weight and abdominal girth of the mice can be inhibited to different degrees, the ascites volume and ascites cell activity are reduced, and the cell apoptosis rate is increased; and the effective part has strong anti-breast cancer activity, and has no obvious toxicity in non-targeted organs, including small intestines, liver and kidney. The effective part can be used for preparing anti-tumor drugs, and a new choice is provided for anti-tumor treatment.

Description

technical field [0001] The present invention relates to the technical field of effective parts of medicinal residues, in particular, to a preparation method of the effective parts of medicinal residues of brucei chinensis containing siberian lactones. Background technique [0002] At present, tumor has become the second leading cause of death in the world after cardiovascular disease, and the number of patients is increasing year by year. Current tumor treatment methods mainly include surgery, radiotherapy, chemotherapy and targeted drug therapy. Conventional surgical excision is difficult to remove tumor cells, so postoperative recurrence is prone to occur; radiotherapy and chemotherapy kill tumor cells and normal cells at the same time, causing great harm to patients; although targeted drug therapy can reduce the effects of drugs. Adverse reactions, but easy to produce tumor drug resistance and lead to tumor recurrence. Traditional Chinese medicine has good advantages in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K33/243A61P35/00A61K133/00
CPCA61K36/185A61K33/243A61P35/00A61K2236/333A61K2236/39A61K2236/53A61K2236/51A61K2300/00
Inventor 陈红英潘碧妍周小琴谢友良欧阳冬生李同根苏子仁
Owner GUANGZHOU BAIYUNSHAN MINGXING PHARM CO LTD
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