Macrophage exclusive chimeric antigen receptor, controllable polarized mononuclear/macrophage for expressing macrophage exclusive chimeric antigen receptor and preparation method and application thereof

A chimeric antigen receptor and macrophage technology, applied in the field of controllable polarized monocyte/macrophage and its preparation, can solve the problems of time-consuming and labor-intensive, difficult transformation, loss of immune function, etc.
CN113402616AActive Publication Date: 2021-09-17ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2021-09-17

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Abstract

The invention provides a macrophage exclusive chimeric antigen receptor, a controllable polarized mononuclear / macrophage for expressing the macrophage exclusive chimeric antigen receptor, and a preparation method and application thereof, and relates to the field of biotechnology. The invention provides a chimeric antigen receptor which comprises an extracellular antigen binding domain, a transmembrane domain and an intracellular activation domain which are sequentially connected, and the extracellular antigen binding domain comprises signal peptide and / or scFv and can specifically recognize cell membrane surface protein EGFRvIII specifically expressed by GBM; the transmembrane domain comprises CD8alpha which linking the extracellular antigen binding domain and the intracellular activation domain; an intracellular activation domain comprises TIR, CD3ZETA or GM-CSFRalpha / beta and promotes polarization of macrophages to M1, the chimeric antigen receptor is introduced into the macrophages, the macrophages are endowed with a targeted killing effect on GBM, and the M1 polarization state of the macrophages is effectively promoted and maintained.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a macrophage-specific chimeric antigen receptor, a controllable polarized monocyte / macrophage expressing the receptor, and a preparation method and application thereof. Background technique

[0002] Glioblastoma (GBM), thought to arise from glial progenitor cells, is the most prevalent and aggressive primary brain tumor ever discovered. Its incidence accounts for 12%-15% of intracranial tumors and 50%-60% of astrocytic tumors. The median survival of patients with this tumor is about 15 months, and the 5-year survival rate is less than 10%. Therefore, this tumor poses a great threat to human life and health. Although the current treatment methods for GBM include surgery, radiotherapy, and chemotherapy, but because the tumor infiltrates and grows deep into the brain lobe, and has the characteristics of rapid growth and easy recurrence, the effect is minimal and it is easy to cause di...

Claims

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